Common genetic variation in the ATP-binding cassette transporter A1, plasma lipids, and risk of coronary heart disease☆

Abstract 

The ATP-binding cassette transporter A-1 (ABCA1) regulates cholesterol efflux from cells and is involved in high-density lipoprotein (HDL) metabolism and atherogenesis. We investigated whether common ABCA1 variants, previously reported to have phenotypic effects in humans, were associated with plasma lipids and CHD in a prospective study of coronary heart disease (CHD) in healthy women.

Three polymorphisms in the promoter region (−565C/T, −191G/C, and −17C/G) and two in the coding region (I883M and R1587K) were genotyped in the Nurses’ Health Study. During 8 years of follow-up, 249 incident cases of CHD were identified and matched to controls (1:2) on age and smoking.

The I883M variant was associated with higher HDL-cholesterol levels among younger women. Nearly complete linkage disequilibrium was observed between −565C/T and −191G/C and their less common alleles predicted a lower risk of CHD (odds ratio of CHD per −191C allele: 0.8; 95% CI, 0.6–1.0). Neither the −17C/G SNP nor the 2 the coding polymorphisms were associated with risk of CHD. The −565C/T and the −191G/C variants were inversely associated with risk of CHD among healthy women, without pronounced effects on plasma lipids.

Keywords: Reverse cholesterol transport, Genetic epidemiology, ATP-binding cassette transporter A1, Coronary heart disease, Plasma lipids