Atherosclerosis
Volume 197, Issue 2 , Pages 549-555 , April 2008

Phytoestrogen α-zearalanol inhibits homocysteine-induced endothelin-1 expression and oxidative stress in human umbilical vein endothelial cells

  • Jinhong Duan

      Affiliations

    • Faculty of Basic Medicine, Peking Union Medical College, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing 100005, PR China
    • ,
  • Haishan Xu

      Affiliations

    • Faculty of Basic Medicine, Peking Union Medical College, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing 100005, PR China
    • ,
  • Shunling Dai

      Affiliations

    • Faculty of Basic Medicine, Peking Union Medical College, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing 100005, PR China
    • Corresponding Author InformationCorresponding author.
    • ,
  • Xiaoming Wang

      Affiliations

    • Faculty of Basic Medicine, Peking Union Medical College, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing 100005, PR China
    • ,
  • Yunqing Wu

      Affiliations

    • Faculty of Basic Medicine, Peking Union Medical College, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing 100005, PR China
    • ,
  • Yandong Zhang

      Affiliations

    • Faculty of Basic Medicine, Peking Union Medical College, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing 100005, PR China
    • ,
  • Renyu Sun

      Affiliations

    • Faculty of Basic Medicine, Peking Union Medical College, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing 100005, PR China
    • ,
  • Jun Ren

      Affiliations

    • Faculty of Basic Medicine, Peking Union Medical College, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing 100005, PR China
    • School of Pharmacy, University of Wyoming, Laramie, WY 82071, USA
    • Corresponding Author InformationCorresponding author at: School of Pharmacy, University of Wyoming, Laramie, WY 82071, USA. Tel.: +1 307 766 6131; fax: +1 307 766 2953.

Received 13 March 2007 ,Revised 14 June 2007 ,Accepted 3 August 2007.

References 

  1. Austin RC, Lentz SR, Werstuck GH. Role of hyperhomocysteinemia in endothelial dysfunction and atherothrombotic disease. Cell Death Differ. 2004;11:S56–S64
  2. Zak A, Zeman M. Consequences of moderate hyperhomocysteinemia in internal medicine. Casopis Lekaru Ceskych. 2004;143:367–374
  3. Weiss N, Heydrick SJ, Postea O, et al. Influence of hyperhomocysteinemia on the cellular redox state—impact on homocysteine-induced endothelial dysfunction. Clin Chem Lab Med. 2003;41:1455–1461
  4. Tsen CM, Hsieh CC, Yen CH, Lau YT. Homocysteine altered ROS generation and NO accumulation in endothelial cells. Chin J Physiol. 2003;46:129–136
  5. Kerlikowske K, Miglioretti DL, Ballard-Barbash R, et al. Prognositc characteristics of breast cancer among postmenopausal hormone users in a screened population. J Clin Oncol. 2003;21:4314–4321
  6. Dai S, Duan J, Lu Y, et al. α-Zearalanol, a phytoestrogen for cardiovascular therapy. Endocrine. 2004;25:117–120
  7. Liew R, Macleod KT, Collins P. Novel stimulatory actions of the phytoestrogen genistein: effects on the gain of cardiac excitation-contraction coupling. FASEB J. 2003;17:1307–1309
  8. Jaffe EA, Nachman RL, Becker CG, Minick CR. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic an dimmunologic criteria. J Clin Invest. 1973;52:2745–2756
  9. Xu HS, Duan J, Dai S, et al. α-Zearalanol antagonizes oxidized LDL-induced inhibition of nitric oxide production and stimulation of endothelin-1 release in human umbilical vein endothelial cells. Endocrine. 2004;25:235–246
  10. Weiss N, Keller C, Hoffmann U, Loscalzo J. Endothelial dysfunction and atherothrombosis in mild hyperhomocysteinemia. Vasc Med. 2002;7:227–239
  11. Drunat S, Moatti N, Paul JL, et al. Homocysteine-induced decrease in endothelin-1 production is initiated at the extracellular level and involves oxidative products. Eur J Biochem. 2001;268:5287–5294
  12. McCully KS, Wilson RB. Homocysteine theory of arteriosclerosis. Atherosclerosis. 1975;22:215–227
  13. Weiss N. Mechanisms of increased vascular oxidant stress in hyperhomocys-teinemia and its impact on endothelial function. Curr Drug Metab. 2005;6:27–36
  14. Topal G, Brunet A, Millanvoye E, et al. Homocysteine induces oxidative stress by uncoupling of NO synthase activity through reduction of tetrahydrobiopterin. Free Radic Biol Med. 2004;36:1532–1541
  15. Seshadri S. Elevated plasma homocysteine levels: risk factor or risk marker for the development of dementia and Alzheimer's disease?. J Alzheimers Dis. 2006;9:393–398
  16. Dai S, Duan J, Lu Y, et al. α-Zearalanol inhibits the progression of atherosclerosis and improves lipid profile in ovariectomized cholesterol-fed rabbits. Endocrine. 2004;25:121–130
  17. Liu JC, Chen JJ, Chan P, Cheng CF, Cheng TH. Inhibition of cyclic strain-induced endothelin-1 gene expression by resveratrol. Hypertension. 2003;42:1198–1205
  18. Ji ES, Li Q, He RR. Action of genistein on tension of isolated rabbit femoral artery and its mechanism. Sheng Li Xue Bao. 2002;54:422–426
  19. Cheng CM, Hong HJ, Liu JC, et al. Crucial role of extracellular signal-regulated kinase pathway in reactive oxygen species-mediated endothelin-1 gene expression induced by endothelin-1 in rat cardiac fibroblasts. Mol Pharmacol. 2003;63:1002–1011
  20. Lu Z, Xu S. ERK1/2 MAP kinases in cell survival and apoptosis. IUBMB Life. 2006;58:621–631

PII: S0021-9150(07)00522-9

doi: 10.1016/j.atherosclerosis.2007.08.018

Atherosclerosis
Volume 197, Issue 2 , Pages 549-555 , April 2008

Article Tools

* Image Usage & Resolution