Atherosclerosis
Volume 132, Issue 1 , Pages 53-59, 11 July 1997

Inhibition of smooth muscle cell migration by the p21 cyclin-dependent kinase inhibitor (Cip1)

  • Ryousuke Fukui

      Affiliations

    • First Department of Internal Medicine, Osaka Medical College, 2-7 Daigaku-cho, Takatsuki-city, Osaka, 569, Japan
    • Corresponding Author InformationCorresponding author. Tel.: +81 726 831221; fax: +81 726 831805
    • ,
  • Nobuhiko Shibata

      Affiliations

    • First Department of Internal Medicine, Osaka Medical College, 2-7 Daigaku-cho, Takatsuki-city, Osaka, 569, Japan
    • ,
  • Eiko Kohbayashi

      Affiliations

    • First Department of Internal Medicine, Osaka Medical College, 2-7 Daigaku-cho, Takatsuki-city, Osaka, 569, Japan
    • ,
  • Masahiro Amakawa

      Affiliations

    • Kaken Pharmaceutical Company, Limited, Yamashina-ku, Kyoto, 607, Japan
    • ,
  • Daisuke Furutama

      Affiliations

    • First Department of Internal Medicine, Osaka Medical College, 2-7 Daigaku-cho, Takatsuki-city, Osaka, 569, Japan
    • ,
  • Masaaki Hoshiga

      Affiliations

    • First Department of Internal Medicine, Osaka Medical College, 2-7 Daigaku-cho, Takatsuki-city, Osaka, 569, Japan
    • ,
  • Nobuyuki Negoro

      Affiliations

    • First Department of Internal Medicine, Osaka Medical College, 2-7 Daigaku-cho, Takatsuki-city, Osaka, 569, Japan
    • ,
  • Takahiro Nakakouji

      Affiliations

    • First Department of Internal Medicine, Osaka Medical College, 2-7 Daigaku-cho, Takatsuki-city, Osaka, 569, Japan
    • ,
  • Masaaki Ii

      Affiliations

    • First Department of Internal Medicine, Osaka Medical College, 2-7 Daigaku-cho, Takatsuki-city, Osaka, 569, Japan
    • ,
  • Tadashi Ishihara

      Affiliations

    • First Department of Internal Medicine, Osaka Medical College, 2-7 Daigaku-cho, Takatsuki-city, Osaka, 569, Japan
    • ,
  • Nakaaki Ohsawa

      Affiliations

    • First Department of Internal Medicine, Osaka Medical College, 2-7 Daigaku-cho, Takatsuki-city, Osaka, 569, Japan

Received 12 September 1996; received in revised form 12 February 1997; accepted 19 March 1997.

Abstract 

In vascular smooth muscle cells (SMCs), proliferation and migration contribute to lesion formation after arterial injury. In the cell cycle, several cyclin-dependent kinases (cdks) inhibitors are implicated in the regulating of cyclin–cdk activity such as p21Cip1, p16Ink4 and p27Kip1. Although Cip1 inhibits SMC proliferation, its effects on SMC migration are unknown. To test the hypothesis that Cip1 inhibits SMCs migration and proliferation, we transfected the Cip1 gene into a strain of rabbit aortic SMCs (SM3 cells). Both the spreading and the attachment of Cip1-transfected SM3 cells to extracellular matrices (ECMs) were inhibited compared to that of vector-transfected cells. In the modified Boyden's chamber assay the effect of fibronectin on the migratory activity of Cip1-transfected SM3 cells was significantly less than that of vector transfected cells in response to PDGF-BB. These data suggested that Cip1 inhibited both the migration and proliferation of SMC.

Keywords:  p21Cip1, Smooth muscle cells, Migration, Adhesion

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PII: S0021-9150(97)00086-5

Atherosclerosis
Volume 132, Issue 1 , Pages 53-59, 11 July 1997

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