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Aortic lipogenesis during aerobic and hypoxic incubation

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      Abstract

      Atherosclerotic rabbit aortas incorporate more [2-14C]glucose into lipids when incubated under hypoxic than aerobic conditions. This increase results from greater radiosubstrate incorporation into the glycerol moiety of glycerides and phospholipids. Fatty acids are synthesized mainly by elongation; synthesis of all fatty acids is less during hypoxia. Though the total amount of [1-14C]acetate incorporated into lipids is the same during both incubation conditions, there is a redistribution so that this substrate equilibrates more into phospholipid glycerol during hypoxia. Fatty acid synthesis from [1-14C]acetate is primarily by elongation and decreases during hypoxic incubation.

      Keywords

      Abbreviations:

      TLC (thin-layer chromatography), GLC (gas-liquid chromatography)
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