The measurement of platelet aggregation in small blood samples

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      A simple method is described for measuring platelet aggregation rapidly and objectively in 0.5 ml samples of whole blood. Aggregation is induced by adenosine diphosphate, 5-hydroxytryptamine, connective tissue extract or glass beads. The samples are mixed at 37°, and the unaggregated platelets in the supernatant are counted after adding an equal volume of Eagle's medium to each sample. Concentration-dependent aggregation responses are obtained, and the reproducibility of tests performed several days or weeks apart on the same subject is satisfactory. This technique is being used for long-term studies on man and laboratory animals.


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