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The microdissection of large atherosclerotic plaques to give morphologically and topographically defined fractions for analysis

Part 2. Studies on “nile blue” cells
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      Abstract

      Cells staining intensely blue with Nile blue sulphate are a striking feature of some human atherosclerotic lesions, and it has been suggested that they are “phospholipid-containing macrophages”. Nile blue cells were isolated by microdissection from 24 raised lesions and their extracted lipids were compared with the lipids in adjacent tissue samples. No large amount of phospholipid could be demonstrated; the concentration of phospholipid was only slightly greater than the concentration in fibrous tissue and was less than that in other fat-filled cells.
      The Nile blue staining material was fluorescent, acid fast, sudanophilic and resistant to extraction by lipid solvents and thus in many respects resembled lipofuscin. Rings of Nile blue staining material partly obscured by the amorphous lipid were revealed on extraction of sections with methanol : chloroform (50 : 50, v/v).
      The distribution of Nile blue cells was related to morphology rather than to age. They were not found in normal intima at any age (157 samples, age range 12–70) but occurred in 87% of atherosclerotic lesions with thick fibrous caps. By contrast, in fatty streaks and plaques containing numerous fat-filled cells they occurred in only 20–25% of samples.

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      References

        • Cain A.J.
        The use of Nile blue in the examination of lipoids.
        Quart. J. Microsc. Sci. 1947; 88: 383
        • Dunnigan M.G.
        The distribution of phospholipid within macrophages in human atheromatous plaques.
        J. Atheroscler. Res. 1964; 4: 144
        • Dunnigan M.G.
        The Histochemistry of Lipids in Human Atherosclerctic Plaques with Special Reference to Phospholipids.
        M.D. Thesis. 1969; (Glasgow)
        • Smith E.B.
        • Slater R.S.
        The microdissection of large atherosclerotic plaques to give morphologically and topographically defined fractions for analysis, Part 1 (The lipids in the isolated fractions).
        Atherosclerosis. 1972; 15: 37-56
        • Chiffelle T.L.
        • Putt F.A.
        Propylene and ethylene glycol as solvent for Sudan IV and Sudan black B.
        Stain Technol. 1951; 26: 51
        • Pearse A.G.E.
        3rd edition. Histochemistry, Theoretical and Applied. Vol. 1. Churchill, London1968: 640
        • Adams C.W.M.
        A histochemical method for the simultaneous demonstration of normal and degenerating myelin.
        J. Pathol. Bacteriol. 1959; 77: 648
        • Adams C.W.M.
        Acid haematein method for choline-containing phospholipids (modified after Baker, 1946, 1947).
        in: Adams C.W.M. Neurohistochemistry. Elsevier, Amsterdam1965: 51
        • Landing B.H.
        • Uzman L.L.
        • Whipple A.
        Phosphomolybdic acid as a staining reagent for lipids.
        Lab. Invest. 1952; 1: 456
        • Pearse A.G.E.
        Histochemistry, Theoretical and Applied.
        in: 2nd edition. Churchill, London1961: 661-665
        • McManus J.F.A.
        Histological demonstration of mucin after periodic acid.
        Nature. 1946; 158: 202
        • Belt W.D.
        • Hayes E.R.
        An ultra-violet Schiff reaction for unsaturated lipids.
        Stain Technol. 1956; 31: 117
        • Lillie R.D.
        Ethylenic reaction of ceroid with performic acid and Schiff reagent.
        Stain Technol. 1952; 27: 37
        • Hayes E.R.
        A rigorous re-definition of the plasmal reaction.
        Stain Technol. 1949; 24: 19
        • Smith E.B.
        • Slater R.S.
        • Chu P.K.
        The lipids in raised fatty and fibrous lesions in human aorta. A comparison of the changes at different stages of development.
        J. Atheroscler. Res. 1968; 8: 399
        • Smith E.B.
        • Evans P.H.
        • Downham M.D.
        Lipid in the aortic intima. The correlation of morphological and chemical characteristics.
        J. Atheroscler. Res. 1967; 7: 171
        • Strehler B.L.
        On the histochemistry and ultrastructure of age pigment.
        Advan. Gerontol. Res. 1964; 1: 343
        • Samorajski T.
        • Ordy J.M.
        The histochemistry and ultrastructure of lipid pigment in the adrenal glands of ageing mice.
        J. Gerontol. 1967; 22: 253
        • Björkerud S.
        Isolated lipofuscin granules. A survey of a new field.
        Advan. Gerontol. Res. 1964; 1: 257
        • Adams C.W.M.
        Histochemistry of atherosclerosis correlated with structural and chemical pathology.
        in: Adams C.W.M. Vascular Histochemistry. Lloyd-Luke, London1967: 115
        • Burt R.C.
        The incidence of acid-fast pigment (ceroid) in aortic atherosclerosis.
        Am. J. Clin. Pathol. 1952; 22: 135
        • Pappenheimer A.M.
        • Victor J.
        “Ceroid” pigment in human tissues.
        Am. J. Pathol. 1946; 22: 395
        • Hartcroft W.S.
        Pathogenesis and significance of haemoceroid and hyaloceroid, two types of ceroid like pigments found in human atheromatous lesions.
        J. Gerontol. 1953; 8: 158
        • Gyorkey F.
        • Shimamura T.
        • O'Neal R.M.
        The fine structure of ceroid in human atheroma.
        J. Histochem. Cytochem. 1967; 15: 732
        • Strehler B.L.
        • Mark D.D.
        • Mildvan A.S.
        • Gee M.V.
        Rate and magnitude of age pigment accumulation in the human myocardium.
        J. Gerontol. 1959; 14: 430