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Enzyme histochemical observations on the effect of pyridinol carbamate on cholesterol-induced atherosclerosis

  • M. Mottonen
    Affiliations
    Department of Forensic Medicine (Head: Professor Jyrki Raekallio), Department of Pathological Anatomy (Head: Professor Osmo Järvi), University of Turku, and Medical Research Laboratories (Lääke Ov and Medipolar Oy) (Head: Professor Kaarlo Hartiala), Turku Finland
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  • M. Pantio
    Affiliations
    Department of Forensic Medicine (Head: Professor Jyrki Raekallio), Department of Pathological Anatomy (Head: Professor Osmo Järvi), University of Turku, and Medical Research Laboratories (Lääke Ov and Medipolar Oy) (Head: Professor Kaarlo Hartiala), Turku Finland
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  • L. Nieminen
    Affiliations
    Department of Forensic Medicine (Head: Professor Jyrki Raekallio), Department of Pathological Anatomy (Head: Professor Osmo Järvi), University of Turku, and Medical Research Laboratories (Lääke Ov and Medipolar Oy) (Head: Professor Kaarlo Hartiala), Turku Finland
    Search for articles by this author
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      Abstract

      The effect of pyridinol carbamate on cholesterol-induced atherosclerosis in the rabbit was investigated in 28 animals. The diet was given for 8 months. The effect of pyridinol carbamate on lactate-NAD-tetrazolium reductase and ATPase activities of the abdominal and thoracic aortae was examined histochemically.
      The animals were divided into 4 dietary groups:
      • 1.
        1. regular diet (commercial rabbit food pellets, Orion)
      • 2.
        2. regular diet + pyridinol carbamate (100 mg/kg)
      • 3.
        3. regular diet + cholesterol (1 %)
      • 4.
        4. regular diet + cholesterol (1 %) + pyridinol carbamate (100 mg/kg).
      Lactate-NAD-tetrazolium reductase and ATPase were identical in the samples taken from the thoracic and abdominal aortae in all groups.
      No atherosclerotic formation was noted in dietary groups 1 and 2. Lactate-NAD-tetrazolium reductase and ATPase activities were identical in these two groups.
      Pyridinol carbamate was not observed to have any effect on lactate-NAD-tetrazolium reductase and ATPase activities of the aorta and atheroma plaques of the rabbit.

      Keywords

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