Research paper| Volume 15, ISSUE 2, P141-145, March 1972

Lipoprotein synthesis in chickens FED cholesterol

      This paper is only available as a PDF. To read, Please Download here.


      l-[14C]Leucine was used to investigate the synthesis of serum lipoproteins in normal and cholesterol-fed chickens. No radioactivity was present in the fasting serum VLDL fraction from normal birds while a substantial amount of radioactivity remained in the same fraction from cholesterol-fed animals, even 4 h after the injection of label. The total incorporation of the label into LDL, HDL and serum proteins was not influenced by the inclusion of cholesterol in the diet. Almost no activity was found in the VLDL fraction of normal unfasted chickens during a 2 h period after the injection of label, whereas a steadily increasing amount of radioactivity was observed during the same time interval in the cholesterol-fed birds. As one possible explanation, it was proposed that the large increase in the VLDL fraction observed in the cholesterol-fed chickens as compared to the normal birds was due to a decreased rate of removal of this lipoprotein from circulation.


      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'


      Subscribe to Atherosclerosis
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


        • Jones R.J.
        • Dobrilovic L.
        Aortic cholesterol and the plasma lipoproteins of the cholesterol-fed cockerel.
        in: Proc. Soc. exp. Biol. Med.130. 1969: 163
        • Leveille G.A.
        • Tillotson J.A.
        • Sauberlich H.E.
        Fatty acid composition of plasma and liver lipid components as influenced by diet in the growing chick.
        J. Nutr. 1963; 81: 357
        • Leveille G.A.
        • Sauberlich H.E.
        Lipid changes in plasma alpha lipoproteins, liver and aorta of chicks fed different fats.
        in: Proc. Soc. exp. Biol. Med.112. 1963: 300
        • Wilcox H.G.
        • Heimberg M.
        Isolation of plasma lipoproteins by zonal ultracentrifugation in the B14 and B15 titanium rotors.
        J. Lipid Res. 1970; 11: 7
        • Hillyard L.A.
        • White H.M.
        Characterization of chicken serum lipoproteins.
        in: Fed. Proc.28. 1969: 447
        • Freid M.
        • Wilcox H.G.
        • Faloona G.R.
        • Eoff S.P.
        • Hoffman M.S.
        • Zimmerman D.
        The biosynthesis of plasma lipoproteins in higher animals.
        Comp. Biochem. Physiol. 1968; 25: 651
        • Havel R.J.
        • Eder H.A.
        • Bragdon J.H.
        The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum.
        J. clip. Invest. 1955; 34: 1345
        • Kruski A.W.
        Influence of Cholesterol on the Concentration, Composition and Synthesis of Chicken Serum Lipoprotein.
        in: Ph. D. Thesis. University of Illinois, Urbana1971
        • Hatch F.T.
        • Lees R.S.
        Practical methods for plasma lipoprotein analysis.
        Adv. Lipid Res. 1968; 6: 1
        • French J.E.
        • Robinson D.S.
        • Harris P.M.
        The role of cholesterol in the interaction of chyle and plasma.
        Quart. J. exp. Physiol. 1955; 40: 320
        • Korn E.D.
        • Quigley Jr., T.W.
        Lipoprotein lipase of chicken adipose tissue.
        J. Biol. Chem. 1957; 226: 833
        • Whitson D.
        • Carrick C.W.
        • Roberts R.E.
        • Hauge S.M.
        Utilization of fat by chickens — A method for determining the absorption of nutrients.
        Poultry Sci. 1943; 22: 137