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Lipoprotein synthesis in chickens FED cholesterol

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      Abstract

      l-[14C]Leucine was used to investigate the synthesis of serum lipoproteins in normal and cholesterol-fed chickens. No radioactivity was present in the fasting serum VLDL fraction from normal birds while a substantial amount of radioactivity remained in the same fraction from cholesterol-fed animals, even 4 h after the injection of label. The total incorporation of the label into LDL, HDL and serum proteins was not influenced by the inclusion of cholesterol in the diet. Almost no activity was found in the VLDL fraction of normal unfasted chickens during a 2 h period after the injection of label, whereas a steadily increasing amount of radioactivity was observed during the same time interval in the cholesterol-fed birds. As one possible explanation, it was proposed that the large increase in the VLDL fraction observed in the cholesterol-fed chickens as compared to the normal birds was due to a decreased rate of removal of this lipoprotein from circulation.

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