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Quantitative studies on fibrinogen and low-density lipoprotein in human aortic intima

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      Abstract

      The amounts of soluble, fibrinogen/fibrin related antigens (FRA) and of intact low-density (LD) lipoprotein in human aortic intima have been measured by an immunoelectrophoretic technique. Substantial amounts of FRA and LD lipoprotein were found in normal intima: in early fibrous lesions the concentrations of both antigens showed two- to four-fold increases compared with normal intima from the same aorta.
      In spite of the increase in concentration the ratio LD lipoprotein cholesterol/FRA did not differ significantly between normal intima and lesions. There was a significant correlation between lipoprotein and FRA (r = 0.722, P = 0.015), which suggests that fibrinogen may be entering the intima together with lipoprotein and other plasma constituents. When tissue samples were treated with thrombin about 50% of the antigen was “clotted”; the “clottable” material was presumably fibrinogen since “clottable” fragments are not derived from lysis of a stabilized fibrin clot. The results suggest that substantial amounts of plasma fibrinogen enter the intima; if this is converted to fibrin within the intimal tissue it could be a potent factor in atherogenesis.

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      References

        • Duguid J.B.
        Thrombosis as a factor in the pathogenesis of coronary atherosclerosis.
        J. Pathol. Bacteriol. 1946; 58: 207
        • Duguid J.B.
        Thrombosis as a factor in the pathogenesis of aortic atherosclerosis.
        J. Pathol. Bacteriol. 1948; 60: 57
        • Constantinides P.
        Plaque fissures in human coronary thrombosis.
        J. Atheroscler. Res. 1966; 6: 1
        • Woolf N.
        • Sacks M.I.
        • Davis M.J.
        Aortic plaque morphology in relation to coronary artery disease.
        Amer. J. Pathol. 1969; 57: 187
        • Haust M.D.
        • Wyllie J.C.
        • More R.H.
        Demonstration of fibrin in the white plaque by the fluorescent antibody technique.
        Amer. J. Pathol. 1964; 44: 225
        • Kao V.
        • Wissler R.W.
        A study of the immunohistochemical localization of serum lipoproteins and other plasma proteins in human atherosclerotic lesions.
        J. Exp. Molec. Pathol. 1965; 4: 465
        • Walton K.W.
        • Williamson N.
        Histological and immunofluorescent studies on the evolution of the human atheromatous plaque.
        J. Atheroscler. Res. 1968; 8: 599
        • Smith E.B.
        • Slater R.S.
        The relationship between low density lipoprotein in the aortic intima and the patient's serum lipids.
        Lancet. 1972; i: 463
        • Page I.H.
        Atherosclerosis: an introduction.
        Circulation. 1954; 10: 1
        • Nilehn J.E.
        Separation and estimation of split “products” of fibrinogen and fibrin in human serum.
        Thromb. Diath. Haemorrh. 1967; 18: 487
        • Bouma B.N.
        A modification of the immunochemical determination of split products described by Nilehn.
        Scand. J. Haematol. 1971; : 391
        • Smith E.B.
        • Slater R.S.
        Lipids and low density lipoproteins in intima in relation to its morphological characteristics.
        in: Atherogenesis: Initiating Factors. Ciba Symposium No. 12. Associated Scientific Publishers, Amsterdam1973: 39
        • Geer J.C.
        • Haust M.D.
        Smooth muscle cells in atherosclerosis.
        in: Monographs on Atherosclerosis. Vol. 2. Karger, Basle1972
        • Benkö A.
        • Laki K.
        Studies on the clot stabilizing enzyme in aorta of rabbits under normal conditions and after cholesterol feeding.
        Biochem. Biophys. Res. Commun. 1968; 11: 14
        • Gaffney P.J.
        Molecular aspects of fibrin clot solubilization.
        Nature New Biol. 1971; 234: 281
        • Marder V.J.
        • Shulman N.R.
        • Carroll W.R.
        High molecular weight derivatives of human fibrinogen produced by plasmin.
        J. Biol. Chem. 1969; 244: 2111
        • Fletcher A.P.
        • Alkjaersig N.
        • Fisher S.
        • Sherry S.
        The proteolysis of fibrinogen by plasmin: the identification of thrombin-clottable fibrinogen derivatives which polymerize abnormally.
        J. Lab. Clin. Med. 1966; 68: 780
        • Smith E.B.
        • Slater R.S.
        The chemical and immunological assay of low density lipoproteins extracted from human aortic intima.
        Atherosclerosis. 1970; 11: 417
        • Sternby N.H.
        Atherosclerosis in a defined population.
        Acta Pathol. Microbiol. Scand. 1968;
        • Haust M.D.
        Electron microscopic and immuno-histochemical studies of fatty streaks in human aorta.
        Progr. Biochem. Pharmacol. 1968; 4: 429
        • Porter K.R.
        • Hawn C.v.Z.
        The culture of tissue cells in clots formed from purified bovine fibrinogen and thrombin.
        in: Proc. Soc. Exp. Biol. (N.Y.). 65. 1947: 309