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Abstract
The lipoproteins with density less than 1.019 g/ml (VLDL) of rabbits made hypercholesterolemic
by cholesterol feeding were fractionated by gel filtration. The proportion of the
two fractions obtained (VLDL-1 and VLDL-2) was found to be dependent on the degree
of hypercholesterolemia. Significant differences in the percentage composition, size
and flotation rate of the two fractions were found but not in their apolipoproteins.
By injecting into control animals the two VLDL fractions with their cholesterol moiety
labelled with radioactive carbon, it was found that their rates of disappearance from
plasma were different. No radioactivity was detected in plasma one hour after the
injection of the VLDL subclass made up of the larger particles (VLDL-1). At shorter
times, cholesterol injected as VLDL-1 was found associated with VLDL-2. However, no
radioactivity was present as VLDL-1 when labelled VLDL-2 was injected. The cholesterol
moiety of VLDL-l was more easily extracted by shaking with hexane than that of VLDL-2
or LDL. The results are discussed in terms of the possible relationships between the
structural properties of the abnormal lipoproteins, their physiochemical stability
and metabolic fate.
Keywords
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Article info
Publication history
Received:
March 15,
1973
Identification
Copyright
© 1974 Published by Elsevier Inc.