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The effect of massive doses of ascorbic acid and methionine on the levels of lipids and glycosaminoglycans in the aorta of weanling rats

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      Abstract

      The levels of the glycosaminoglycans, hyaluronic acid (HA), heparan sulphate (HS), chondroitin sulphate A (Ch S-A), chondroitin sulphate C (Ch S-C), chondroitin sulphate B (Ch S-B) and heparin (H) increased in the aorta in weanling rats fed ascorbic acid or ascorbic acid + methionine. HS and Ch S-A decreased more in animals that were fed ascorbic acid alone, while Ch S-C, Ch S-B and H increased more with ascorbic acid + methionine. The cholesterol level of the serum, liver and aorta decreased with ascorbic acid, and more so with ascorbic acid + methionine. Liver phospholipids increased, and again more so with ascorbic acid + methionine. Triglyceride in the aorta and liver considerably decreased in the animals fed ascorbic acid + methionine.

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      References

        • Kasatkina L.V.
        • Lobova N.M.
        • Sukasova M.I.
        Myasnikov A.L. Atherosclerosis and Thrombosis. Mir Publishers, Moscow1967: 39
        • Zubova R.F.
        Effect of vitamin C and B6 level in blood of atherosclerotic patients.
        Serd-Sosud Patol (Kiev: Zdorovc) Sb. 1964; : 165
        • Inagaki C.
        • Oizumi K.
        Effect of ascorbic acid on cholesterol metabolism.
        Vitamin. 1965; 32: 339
        • Volkova K.G.
        Effect of ascorbic acid on total lipids in the aortic wall in experimental cholesterol atherosclerosis.
        Ateroskleroz. Sb. 1961; : 187
        • Catelli P.
        • Bignami M.
        • Bonati G.
        • Gofrini M.
        Changes in serum cholesterol and lipoproteins in subjects treated with vitamin C.
        Pathologica. 1961; 53: 267
        • Smolenskii V.S.
        • Erofeeva N.N.
        • Pankratova N.F.
        • Zaprometov M.N.
        Influence of vitamin C and vitamin P administration on the development of atherosclerosis.
        Vit Resury i ikh Ispolz Akad. Nauk SSR, Inst. Biokhim Sb. 1959; 4: 158
        • Cortinovis R.
        • Silenzi C.
        • Farroni L.
        Changes induced by l-ascorbic acid in the blood cholesterol in normal and atherosclerotic subjects.
        Giorn. Gerontol. 1960; 8: 28
        • Silenzi C.
        • Cortinovis A.
        • Farroni L.
        Acute changes in lipoproteinograms by administration of ascorbic acid.
        Giorn. Gerontol. 1960; 8: 32
        • De Soldati Leon
        • Balassanian S.
        • Striztler G.
        Blood lipid levels in patients with atherosclerosis under treatment with ascorbic acid.
        Prensa Med. Arg. 1960; 47: 636
        • Gorbachev V.V.
        Effect of some vitamins on lipid metabolism of atherosclerotic patients.
        Zdravookhranenic Belorussii. 1959; 12: 22
        • Chang S.C.
        Effect of mechanical trauma, cortisone and vitamin C upon atherosclerosis in cholesterol-fed rats.
        New Med. J. 1965; 8: 79
        • Kosko N.K.
        • Litvishkova L.A.
        Effect of vitamin P and C on the cholesterol level in patients with atherosclerosis.
        Gipertonich. Bolezni, Atheroscleroz, Koronarn, Nedostatochnost. Sb. 1963; : 305
        • Samuel P.
        • Shalchi O.B.
        Effect of vitamin C on serum cholesterol in patients with hypercholesterolaemia and arteriosclerosis.
        Circulation. 1964; 29: 24
        • Nakamura T.
        • Tokita K.
        • Tateno S.
        • Kotoku T.
        • Ohba T.
        Human aortic acid mucupolysaccharides and glycoproteins: Changes during ageing and in atherosclerosis.
        J. Atheroscler. Res. 1968; 8: 891
        • Kumar V.
        • Berenson G.S.
        • Ruiz H.
        • Dalferes Jr., E.R.
        • Strong J.P.
        Acid mucopolysaccharides of human aorta, Part 2 (Variations with atherosclerotic involvement).
        J. Atheroscler. Res. 1967; 7: 583
        • Ichida T.
        • Kalant N.
        Aortic glycosaminoglycans in atheroma and alloxan diabetes.
        Canad. J. Biochem. 1968; 46: 249
        • Mullinger R.N.
        • Manley G.
        Glycosaminoglycans and atherosclerosis in animal aortas.
        J. Atheroscler. Res. 1969; 9: 108
        • Böttcher C.J.F.
        • Klynstra F.B.
        Acid mucopolysaccharides in human aortic tissue (The distribution at different stages of atherosclerosis).
        Lancet. 1963; ii: 439
        • Seethanathan P.
        • Kurup P.A.
        Changes in tissue glycosaminoglycans in rats fed a hypercholesterolaemic diet.
        Atherosclerosis. 1971; 14: 65
        • Gore I.
        • Tanaka Y.
        • Fujinami T.
        • Goodman M.L.
        Aortic acid mucopolysaccharides and collagen in scorbutic guinea pigs.
        J. Nutr. 1965; 87: 311
        • Burlina A.
        • Lippi V.
        Pathologic histology of the liver in relation to ascorbic acid deficiency.
        Riv. Anat. Patol. Oncol. 1959; 16: XXIV
        • Schultz-Haudt S.D.
        • Sigurd H.
        Comparative histochemistry of non-injured skin connective tissue of normal and ascorbic acid deficient guinea pigs.
        Acta Pathol. Microbiol. Scand. 1964; 62: 465
        • Mumma R.O.
        • Verlangieri A.J.
        Isolation of ascorbic acid-2-sulphate from selected rat organs.
        Biochem. Biophys. Acta. 1972; 273: 249
        • Mumma R.O.
        • Verlangieri A.J.
        In vivo sulphation of cholesterol by ascorbic acid-sulphate as possible explanation for the hypocholesterolaemic effect of ascorbic acid.
        in: 13th edition,. Fed. Proc.30. 1971: 370
        • Hawk P.B.
        • Oser B.L.
        • Summerson W.H.
        Practical Physiological Chemistry.
        in: 13th edition,. McGraw-Hill, New York, N.Y1954: 1375
        • Carr J.J.
        • Drekter I.J.
        Estimation of total cholesterol in the serum.
        Clin. Chem. 1956; 2: 353
        • Zilversmit D.B.
        • Davis A.K.
        Micro-determination of phospholipids.
        J. Lab. Clin. Med. 1950; 35: 155
        • Van Handel E.
        • Zilversmit D.B.
        Micromethod for direct determination of serum triglycerides.
        J. Lab. Clin. Med. 1957; 50: 152
        • Bitter T.
        • Muir H.M.
        A modified uronic acid carbazole reaction.
        Anal. Biochem. 1962; 4: 330
        • Hawk P.B.
        • Oser B.L.
        • Summerson W.H.
        Practical Physiological Chemistry.
        in: 13th edition. McGraw-Hill, New York, N.Y1954: 1
        • Ginter E.
        • Bobek P.
        • Gerbelova M.
        Effect of acute avitaminosis C and prolonged low vitamin C intake on serum lipoprotein patterns in guinea pig.
        Bratislav. Lekarske Listy. 1965; 45-11: 11
        • Ginter E.
        • Bobek P.
        • Gerbelova M.
        Effect of scorbutus and prolonged low intake of vitamin C on serum lipoprotein in guinea pigs.
        Nutr. Dieta. 1965; 7: 103
        • Banerjee S.
        • Bandyopadhyaya A.
        Plasma lipids in scurvy: Effect of ascorbic acid supplement and insulin treatment.
        in: Proc. Soc. Exp. Biol. Med.112. 1963: 372
        • Ginter E.
        • Ondreicka R.
        • Bobek P.
        • Simko V.
        Influence of chronic vitamin C deficiency on fatty acid composition of blood serum, liver, triglycerides and cholesterol esters in guinea pigs.
        J. Nutr. 1969; 99: 261
        • Sokoloff B.
        • Hori M.
        • Saelhof C.
        • MacConnell B.
        • Imai T.
        Effect of ascorbic acid on certain blood fat metabolism factors in animals and man.
        J. Nutr. 1967; 91: 107