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Changes in aortic cholesterol-esterifying activity in rabbits fed cholesterol for three days

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      Abstract

      The effect of 3 days' cholesterol feeding on the incorporation of [1-14C]oleic acid into cholesterol ester and phospholipid has been studied in different portions of rabbit aortas incubated in vitro. No significant increase in the incorporation of [1-14C]oleic acid into phospholipid occurred, nor was there any significant increase in the content of either cholesterol or lipid phosphorus in the aortic intima after 3 days' cholesterol feeding. The incorporation of [1-14C]oleic acid into cholesterol ester by the aortic intima, however, was approximately doubled. When the ratio of incorporation of [1-14C]oleic acid into cholesterol ester to that into phospholipid was used for comparison, the effect of the 3 days' cholesterol feeding in inducing cholesterol ester synthesis in the aorta was shown to be more marked in the aortic arch than in the lower portions of aorta and to be evident in the intima but not in the media.

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