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The role of dietary triglycerides in cholesterol metabolism

  • R. Fraser
    Correspondence
    For reprint requests: Dr. R. Fraser, Christchurch Clinical School, Christchurch, New Zealand.
    Affiliations
    Department of Pathology, University of Sydney, Sydney, Australia
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      Abstract

      Ratios of free cholesterol to cholesteryl esters in thoracic duct lymph from rabbits fed varying amounts of cholesterol and triglycerides were estimated to study the influence of dietary triglycerides on cholesterol metabolism. Lymph was collected from groups of animals 6 hours after feeding a marker of tritiated cholesterol in food containing by weight 0, 0.8 or 2 % cholesterol and 0, 15 or 30% of various triglycerides. At constant levels of dietary cholesterol, the proportion of free cholesterol increased as dietary triglyceride increased. At constant levels of a triglyceride, the proportion of cholesteryl esters increased as dietary cholesterol and total cholesterol in lymph increased. These findings may have metabolic significance since the surface of chylomicrons carries diffusible free cholesterol while their core contains the cholesteryl esters which are metabolized by the liver and perhaps inhibit synthesis of cholesterol by that organ.

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      References

        • Hegsted D.M.
        • McGandy R.B.
        • Myers M.L.
        • Stare F.J.
        Quantitative effects of dietary fat on serum cholesterol in man.
        Amer. J. Clin. Nutr. 1965; 17: 281
        • McGill Jr., H.C.
        • Arias-Stella J.
        • Carbonell L.M.
        • Correa P.
        • et al.
        General findings of the international atherosclerosis project.
        Lab. Invest. 1968; 18: 38
        • Getz G.S.
        • Vesselinovitch D.
        • Wissler R.W.
        A dynamic pathology of atherosclerosis.
        Amer. J. Med. 1969; 46: 657
        • Yoffey J.M.
        • Courtice F.C.
        Lymphatics, Lymph and the Lymphomyeloid Complex.
        Academic Press, London1970
        • Zilversmit D.B.
        The composition and structure of lymph chylomicrons in dog, rat and man.
        J. Clin. Invest. 1965; 44: 1610
        • Fraser R.
        Size and lipid composition of chylomicrons of different Svedberg units of flotation.
        J. Lipid Res. 1970; 11: 60
        • Fraser R.
        • DuBien L.
        • Musil F.
        • Fosslien E.
        • Wissler R.W.
        Transport of cholesterol in thoracic duct lymph and serum of rhesus monkeys fed cholesterol with various food fats.
        Atherosclerosis. 1972; 16: 203
        • Zilversmit D.B.
        • Courtice F.C.
        • Fraser R.
        Cholesterol transport in thoracic duct lymph of the rabbit.
        Atherosclerosis. 1967; 7: 319
        • Abell L.L.
        • Levy B.B.
        • Brodie B.B.
        • Kendall F.E.
        A simplified method for the estimation of total cholesterol in serum and demonstration of its specificity.
        J. Biol. Chem. 1952; 195: 357
        • Snyder F.
        Liquid scintillation radio-assay of thin-layer chromatograms.
        in: Bransome E.D. The Current Status of Liquid Scintillation Counting. Grime & Stratton, New York1970: 248
        • Fosslien E.
        • Musil F.
        Automatic lipid extraction and thin-layer chromatography application with a programmed flow system.
        J. Lipid Res. 1970; 11: 605
        • Moreton J.R.
        Atherosclerosis and alimentary hyperlipemia.
        Science. 1947; 106: 190
        • Fraser R.
        • Cliff W.J.
        • Courtice F.C.
        The effect of dietary fat load on the size and composition of chylomicrons in thoracic duct lymph.
        Quart. J. Exp. Physiol. 1968; 53: 390
        • Fraser R.
        • Courtice F.C.
        The transport of cholesterol in thoracic duct lymph of animals fed cholesterol with varying triglyceride loads.
        Aust. J. Exp. Biol. Med. Sci. 1969; 47: 723
        • Wissler R.W.
        • Geer J.C.
        The Pathogenesis of Atherosclerosis.
        in: Williams & Wilkins, Baltimore, Md1972: vii
        • Sylvén C.
        • Börgstrom B.
        Absorption and lymphatic transport of cholesterol in the rat.
        J. Lipid Res. 1968; 9: 596
        • Vahouny G.V.
        • Treadwell C.R.
        Corparative effects of dietary fatty acids and triglycerides on lymph lipids in the rat.
        Amer. J. Physiol. 1959; 196: 881
        • Klauda H.C.
        • Quackenbush F.W.
        Cholesterol absorption with different fats following thoracic duct cannulation of the rat.
        Lipids. 1970; 5: 142
        • Sylvén C.
        • Börgstrom B.
        Intestinal absorption and lymphatic transport of cholesterol in the rat: influence of the fatty acid chain length of the carrier triglyceride.
        J. Lipid Res. 1969; 10: 351
        • Minari O.
        • Zilversmit D.B.
        Behaviour of dog lymph chylomicron lipid constituents during incubation with serum.
        J. Lipid Res. 1963; 4: 424
        • Hillyard L.A.
        • Chaikoff I.L.
        • Entenman C.
        • Reinhardt W.O.
        Composition and concentration of lymph and serum lipoproteins during fat and cholesterol absorption in the dog.
        J. Biol. Chem. 1958; 233: 838
        • Daskalakis E.G.
        • Chaikoff I.L.
        The significance of esterification in the absorption of cholesterol from the intestine.
        Arch. Biochem. 1955; 58: 373
        • Zilversmit D.B.
        The surface coat of chylomicrons: lipid chemistry.
        J. Lipid Res. 1968; 9: 180
        • Dayton S.
        • Hashimoto S.
        Cholesterol flux and metabolism in arterial tissue and in atheromata.
        Exp. Molec. Pathol. 1970; 13: 253
        • Glomsett J.A.
        The plasma lecithin cholesterol acyltransferase reaction.
        J. Lipid Res. 1968; 9: 155
        • Abdulla Y.H.
        • Orton C.C.
        • Adams C.W.M.
        Cholesterol esterification by transacylation in human and experimental atheromatous lesions.
        Atherosclerosis. 1968; 8: 967
        • Goodman De W.S.
        The metabolism of chylomicron cholesterol ester in the rat.
        J. Clin. Invest. 1962; 41: 1886
        • Quarfordt S.H.
        • Goodman De W.S.
        Metabolism of doubly-labelled chylomicron cholesteryl esters in the rat.
        J. Lipid Res. 1967; 8: 264
        • French J.E.
        • Robinson D.S.
        • Harris P.M.
        The role of cholesterol in the interaction of chyle and plasma.
        Quart. J. Exp. Physiol. 1955; 40: 320
        • Nestel P.J.
        • Havel R.J.
        • Bezman A.
        Metabolism of constituent lipids of dog chylomicrons.
        J. Clin. Invest. 1963; 42: 1313
        • Goodman De W.S.
        Cholesterol ester metabolism.
        Physiol. Rev. 1965; 45: 747
        • Redgrave T.G.
        Formation of cholesterol ester-rich particulate lipid during metabolism of chylomicrons.
        J. Clin. Invest. 1970; 49: 465
        • Bergman E.N.
        • Havel R.J.
        • Wolfe B.M.
        • Bohmer T.
        Quantitative studies of the metabolism of chylomicron triglycerides and cholesterol by liver and extrahepatic tissues of sheep and dogs.
        J. Clin. Invest. 1971; 50: 1831
        • Wisse E.
        An electron microscopic study of the fenestrated endothelial lining of rat liver sinusoids.
        J. Ultrastructure Res. 1970; 31: 125
        • Dietschy J.M.
        • Wilson J.D.
        Regulation of cholesterol metabolism.
        New Engl. J. Med. 1970; 282: 1128
        • Weis H.J.
        • Dietschy J.M.
        Failure of bile acids to control hepatic cholesterogenesis: evidence for endogenous cholesterol feedback.
        J. Clin. Invest. 1969; 48: 2398