The effect of carbon disulphide and atherogenic diet on the development of atherosclerotic changes in rabbits

      This paper is only available as a PDF. To read, Please Download here.


      The influence of chronic CS2 intoxication on lipid spectrum in the aorta and blood and on the grade of atheromatosis of the aorta and coronary vessels was studied in rabbits with experimentally induced prolonged hyperlipemia. Rabbits fed on standard laboratory chow served as controls.
      The following biochemical and morphological alterations due to the atherogenic diet alone and those due to hyperlipemia with parallel CS2 exposure were demonstrated respectively: (a) in blood — an approximate 4- and 5-fold elevation of the total (both free and esterified) serum cholesterol with a simultaneous rise in phospholipid and triglyceride (atherogenic diet alone) fractions; (b) in the aortic arch — a 40 and 100% increase in total cholesterol with a 5- and 10-fold rise in the level of cholesterol esters and an approximately uniform 100% increase in the triglyceride fraction; (c) gross atheromatosis of the aorta and histological coronary lesions, both markedly enhanced as a result of chronic CS2 intoxication.
      Evidence was presented of an enhanced susceptibility of the arterial wall to the atherogenic diet under the influence of chronic CS2 intoxication with special reference to the individual regional susceptibility of arterial coronary vessels.


      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'


      Subscribe to Atherosclerosis
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


        • Wrońska-Nofer T.
        • Nofer J.
        Recherches expérimentales sur les troubles du métabolisme des lipides sous l'influence du sulfure de carbone.
        in: Toxicology of carbon Disulphide, Prague3rd edition. Excerpta Medica Monograph. 1966: 161
        • Wrońska-Nofer T.
        The influence of nicotinic acid upon the disturbances in the lipid metabolism caused by carbon disulphide in rats.
        Int. Arch. Arbeitsmed. 1970; 27: 221
        • Wrońska-Nofer T.
        The influence of low doses of nicotinic acid upon the development of lipid disturbances in rats chronically exposed to carbon disulphide.
        Int. Arch. Arbeitsmed. 1972; 29: 285
        • Wrońska-Nofer T.
        Disturbances of lipids metabolism in rats in dependence upon carbon disulphide concentrations in the air.
        Med. Lavoro. 1973; 64: 8
        • Wrońska-Nofer T.
        The incorporation of 14C-acetate into cholesterol in rats exposed to CS2.
        Biochem. Pharmacol. 1969; 18: 925
        • Wrońska-Nofer T.
        Studies on in vivo incorporation of 14C-acetate and 14C-mevalonate into cholesterol in the liver of rats intoxicated with carbon disulphide.
        Int. Arch. Arbeitsmed. 1975; 34: 221
        • Wrońska-Nofer T.
        • Knobloch K.
        The influence of variation of daily rhythm of exposure upon the magnitude of biological effects: A comparison of X-ray irradiation and carbon disulphide intoxication.
        Bull. Acad. Pol. Sci. 1972; 20: 813
        • Wrońska-Nofer T.
        Effect of carbon disulphide intoxication on fecal excretion of end products of cholesterol metabolism.
        Int. Arch. Occup. Environm. Hlth. 1977; 40: 261
        • Szendzikowski S.
        • Patelski J.
        Influence of carbon disulphide intoxication on the lipolytic and esterolytic activity of the aorta of the rat.
        Pat. Pol. 1963; 14 (in Polish): 157
        • Wrońska-Nofer T.
        • Głuszek B.
        Investigations on the lipolytic activity of the blood serum and the wall of the aorta in rats subjected to intoxication with carbon disulphide.
        Med. Pracy. 1965; 16 (in Polish): 24
        • Wrońska-Nofer T.
        Aortic phospholipids synthesis in experimental carbon disulphide intoxication in rats.
        Int. Arch. Occup. Environm. Hlth. 1976; 36: 229
      1. Wrońska-Nofer, T. and Parke, M., Influence of carbon disulphide on metabolic processes in the aorta wall — Study of the rate of cholesterol synthesis and the rate of influx of [14C]-cholesterol from serum into the aorta wall, Int. Arch. Occup. Environm. Hlth, In press.

      2. Wrońska-Nofer, T., Szendzikowski, S. and Parke, M., Experimental atherosclerosis and hyperlipemia in rats — Influence of alimentary fats, cholesterol and carbon disulphide intoxication, Int. Arch. Occup. Environm. Hlth, In press.

        • Folch J.M.
        • Lees M.
        • Sloane-Stanley G.
        A simple method for the isolation and purification of total lipids from animal tissue.
        J. Biol. Chem. 1957; 226: 497
      3. Szendzikowski, A. and Wrońska-Nofer, T., In preparation.

        • Astrup P.
        • Kjeldsen K.
        • Wanstrup J.
        Enhancing influence of carbon monoxide on the development of atheromatosis in cholesterol-fed rabbits.
        J. Atheroscler. Res. 1967; 7: 343
        • Astrup P.
        • Kjeldsen K.
        • Wanstrup J.
        Effects of carbon monoxide exposure on the arterial walls.
        Ann. N.Y. Acad. Sci. 1970; 174: 294
        • Astrup P.
        Some physiological and pathological effects of moderate carbon monoxide exposure.
        Brit. Med. J. 1972; 2: 447
        • Davies R.F.
        • Topping D.L.
        • Turner D.M.
        The effect of intermittent carbon monoxide exposure on experimental atherosclerosis in the rabbit.
        Atherosclerosis. 1976; 24: 527
        • Nofer J.
        • Chojnowski J.
        • Kawecka M.
        • Kieć E.
        • Wrońska T.
        • Wyszomirska Z.
        Effect of occupational hazard to carbon disulphide on the pathogenesis of atherosclerosis.
        Med. Pracy. 1961; 12 (in Polish): 101
        • Hernberg S.
        • Partanen T.
        • Nordman C.H.
        • Sumari P.
        Coronary heart disease among workers exposed to carbon disulphide.
        Brit. J. Ind. Med. 1970; 27: 313
        • Schilling R.S.F.
        Coronary heart disease in viscose-rayon workers.
        Amer. Heart. J. 1970; 80: 1
        • Maugeri S.
        • Cavalleri A.
        • Maugeri U.
        • Taccola A.
        New aspects in the pathogenetic mechanism of carbon disulphide toxic action.
        Med. Lavoro. 1971; 62: 398
        • Vigliani E.C.
        • Pernis B.
        Klinische und experimentelle Untersuchungen über die durch Schwefelkohlenstoffebedingte Atherosclerose.
        Arch. Gewerbepath. Gewerbehyg. 1955; 14: 190
        • Gavrilescu N.
        • Lillis R.
        Cardiovascular effects of long-extended CS2 exposure.
        in: Toxicology of CS2. Excerpta Medica Found, Amsterdam1967: 167
      4. Laurman, W., Wrońska-Nofer, T. and Szendzikowski, S., The effect of carbon disulphide on development of arterial changes in rabbits, In preparation.

        • Patterni L.
        • Pusic G.
        • Teodori S.
        Intossicante lenta da CS2 e arteriosclerosi da dieta ipercolesterolica del coniglio.
        Fol. Med. 1958; 41: 705
        • Prerovska I.
        • Vanecek R.
        • Kubat K.
        The influence of CS2 upon experimental atherosclerosis of rabbits.
        Acta Univ. Carol. Med. 1961; : 177