Advertisement
Research paper| Volume 31, ISSUE 1, P65-76, September 1978

Lipoprotein metabolism in baboons

Effect of feeding cholesterol-rich diet
DOI:https://doi.org/10.1016/0021-9150(78)90038-2
Lipoprotein metabolism in baboons
Previous ArticleIntravenous fat tolerance in obese Africans with varying grades of carbohydrate tolerance
Next ArticleRelative increase in apolipoprotein CII content of VLDL and chylomicrons in a case with massive type V hyperlipoproteinaemia by nicotinic acid treatment
      This paper is only available as a PDF. To read, Please Download here.

      Abstract

      Cholesterol-fed male baboons were selected as a model of human hyperlipidemia. Four chaired baboons fed a diet rich in cholesterol for 6 months had a 100% increase in whole plasma cholesterol, 22% increase in phospholipids with a 44% decrease in triglyceride. Total proteins of very low (VLDL) and high (HDL) density lipoproteins declined by 40% and 15% respectively. Total proteins of intermediate (LDL) and low (LDL) density lipoproteins, however, increased considerably. The cholesterol/protein and cholesterol/triglyceride ratio increased in all lipoproteins. In a separate experiment two animals were employed to study VLDL turnover. These studies were conducted once while they were on a standard diet and once after they had been on an atherogenic diet for 6 months. Radioactivity from VLDL apolipoprotein B in normolipidemic donors and recipients decayed biphasically with the immediate appearance of a large proportion of it in IDL and LDL. The first phase was much more rapid (Math Eq, 0.53 h) than the second (Math Eq, 20.5 h). Most of the VLDL apolipoprotein B (98.5%) decayed via the rapid first phase with a fractional catabolic rate (FCR) of 0.77 h−1. In cross-over studies, half-life of each phase of VLDL apolipoprotein B decay and FCR depended upon the donor and recipient dietary status. Lowest fractional catabolic rate (0.22 h−1) was observed in case of hypercholesterolemic donor and recipient.

      Keywords

      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Atherosclerosis
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Register: Create an account
      Institutional Access: Sign in to ScienceDirect

      References

        • Kritchevsky D.
        Experimental atherosclerosis.
        in: Paoletti R. Lipid Pharmacology. Academic Press, New York1963: 63
        • Clarkson T.B.
        Animal models for atherosclerosis.
        North Carolina Med. J. 1971; 32: 88
        • Gresham G.A.
        • Howard A.N.
        Vascular lesions in primates.
        Ann. N.Y. Acad. Sci. 1965; 127: 694
        • Strong J.P.
        • McGill M.C.
        Diet and experimental atherosclerosis in baboons.
        Amer. J. Path. 1967; 50: 669
        • Howard A.N.
        The baboon in atherosclerosis research — Comparisons with other species and use in testing drugs affecting lipid metabolism.
        in: Day Charles E. Atherosclerosis Drug Discovery. Plenum Press, New York1976: 77
        • Howard A.N.
        • Patelski J.
        • Dowyer D.W.
        • Gresham G.A.
        Atherosclerosis induced in hypercholesterolemic baboons by immunological injury; and the effects of intravenous polyunsatured phosphatidyl choline.
        Atherosclerosis. 1971; 14: 17
        • Lowry O.H.
        • Rosebrough N.J.
        • Farr A.L.
        • Randall R.J.
        Protein measurement with the Folin phenol reagent.
        J. Biol. Chem. 1951; 193: 265
        • McFarlane A.S.
        Efficient trace labelling of proteins with iodine.
        Nature (Lond.). 1958; 182: 53
        • Bilheimer D.W.
        • Eisenberg S.
        • Levy R.I.
        The metabolism of very low density lipoprotein proteins. Part 1 (Preliminary in vitro and in vivo observations).
        Biochim. Biophys. Acta. 1972; 260: 212
        • Kane J.P.
        A rapid electrophoretic technique for identification of subunit species of apoproteins in serum lipoproteins.
        Anal. Biochem. 1973; 53: 497
        • Kane J.P.
        • Sata T.
        • Hamilton R.L.
        • Havel R.J.
        Apoprotein composition of very low density lipoproteins of human serum.
        J. Clin. Invest. 1975; 56: 1622
      12. 3rd edition. Manual of Laboratory Operations, Lipid Research Clinics Program. Vol. 1. U.S. Government Printing Office, Washington, D.C1975 (DHEW Publication, No. (NIH) 75-628)
        • Bartlett G.R.
        Phosphorus assay in column chromatography.
        J. Biol. Chem. 1959; 234: 466
        • Faergeman O.
        • Sata T.
        • Kane J.P.
        • Havel R.J.
        Metabolism of apoprotein B of very low density lipoproteins in the rat.
        J. Clin. Invest. 1975; 56: 1396
        • Kushwaha R.S.
        • Hazzard W.R.
        Catabolism of very low density lipoproteins in the rabbit — Effect of changing composition and pool size.
        Biochim. Biophys. Acta. 1978; 528: 176
        • Weber K.
        • Osborn M.
        The reliability of molecular weight determinations by dodecylsulphate-polyacrylamide gel electrophoresis.
        J. Biol. Chem. 1969; 244: 4406
        • Matthews C.M.E.
        The theory of tracer experiments with 131I-labelled plasma proteins.
        Phys. Med. Biol. 1957; 2: 36
        • Rodriquez J.
        • Catapano A.
        • Ghiselli G.C.
        • Sirtori C.R.
        Very low density lipoproteins in normal and cholesterol fed rabbits, Part 2 (Metabolism of very low density lipoproteins in rabbits).
        Atherosclerosis. 1976; 23: 85
        • Kushwaha R.S.
        • Hazzard W.R.
        • Engblom J.
        High density lipoprotein metabolism in normolipidemic and cholesterol-fed rabbits.
        Biochim. Biophys. Acta. 1978; 530: 132
        • Eisenberg S.
        • Rachmilewitz D.
        Metabolism of rat plasma very low density lipoprotein, Part 1 (Fate in circulation of the whole lipoprotein).
        Biochim. Biophys. Acta. 1973; 326: 378
        • Eisenberg S.
        • Rachmilewitz D.
        Interaction of rat plasma very low density lipoprotein with lipoprotein-rich (postheparin) plasma.
        J. Lipid Res. 1975; 16: 341
        • Howard A.N.
        • Blaton V.
        • Vandamme D.
        • VanLandschoot N.
        • Petters H.
        Lipid changes in plasma lipoproteins of baboons given an atherogenic diet, Part 3 (A comparison between the plasma of the baboon and chimpanzee given atherogenic diets and lipid changes in human plasma lipoproteins of type II hyperlipoproteinemia).
        Atherosclerosis. 1972; 16: 257
        • Mistry P.
        • Nicholl A.
        • Niehaus C.
        • Christie I.
        • James E.
        • Lewis
        Cholesterol feeding revised.
        Circulation. 1976; 54: 178
        • Applebaum D.
        • Cain J.
        • Hazzard W.
        • Albers J.
        • Cheung M.
        • Kushwaha R.
        Short term cholesterol feeding in humans — Failure to induce β-migrating very low density lipoproteins (β-VLDL).
        Clin. Res. 1977; 25: 158A

      Article info

      Publication history

      Accepted: May 24, 1978
      Received in revised form: May 22, 1978
      Received: March 29, 1978

      Footnotes

      ☆This work has been supported by NIH grants HL-18645 and HL-11775.

      Identification

      DOI: https://doi.org/10.1016/0021-9150(78)90038-2

      Copyright

      © 1978 Published by Elsevier Inc.

      ScienceDirect

      Access this article on ScienceDirect
      We use cookies to help provide and enhance our service and tailor content. To update your cookie settings, please visit the for this site.
      Copyright © 2023 Elsevier Inc. except certain content provided by third parties. The content on this site is intended for healthcare professionals.


      RELX