This paper is only available as a PDF. To read, Please Download here.
Abstract
Cholesterol-fed male baboons were selected as a model of human hyperlipidemia. Four
chaired baboons fed a diet rich in cholesterol for 6 months had a 100% increase in
whole plasma cholesterol, 22% increase in phospholipids with a 44% decrease in triglyceride.
Total proteins of very low (VLDL) and high (HDL) density lipoproteins declined by
40% and 15% respectively. Total proteins of intermediate (LDL) and low (LDL) density
lipoproteins, however, increased considerably. The cholesterol/protein and cholesterol/triglyceride
ratio increased in all lipoproteins. In a separate experiment two animals were employed
to study VLDL turnover. These studies were conducted once while they were on a standard
diet and once after they had been on an atherogenic diet for 6 months. Radioactivity
from VLDL apolipoprotein B in normolipidemic donors and recipients decayed biphasically
with the immediate appearance of a large proportion of it in IDL and LDL. The first
phase was much more rapid (
, 0.53 h) than the second (
, 20.5 h). Most of the VLDL apolipoprotein B (98.5%) decayed via the rapid first phase
with a fractional catabolic rate (FCR) of 0.77 h−1. In cross-over studies, half-life of each phase of VLDL apolipoprotein B decay and
FCR depended upon the donor and recipient dietary status. Lowest fractional catabolic
rate (0.22 h−1) was observed in case of hypercholesterolemic donor and recipient.


Keywords
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to AtherosclerosisAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- Experimental atherosclerosis.in: Paoletti R. Lipid Pharmacology. Academic Press, New York1963: 63
- Animal models for atherosclerosis.North Carolina Med. J. 1971; 32: 88
- Vascular lesions in primates.Ann. N.Y. Acad. Sci. 1965; 127: 694
- Diet and experimental atherosclerosis in baboons.Amer. J. Path. 1967; 50: 669
- The baboon in atherosclerosis research — Comparisons with other species and use in testing drugs affecting lipid metabolism.in: Day Charles E. Atherosclerosis Drug Discovery. Plenum Press, New York1976: 77
- Atherosclerosis induced in hypercholesterolemic baboons by immunological injury; and the effects of intravenous polyunsatured phosphatidyl choline.Atherosclerosis. 1971; 14: 17
- Protein measurement with the Folin phenol reagent.J. Biol. Chem. 1951; 193: 265
- Efficient trace labelling of proteins with iodine.Nature (Lond.). 1958; 182: 53
- The metabolism of very low density lipoprotein proteins. Part 1 (Preliminary in vitro and in vivo observations).Biochim. Biophys. Acta. 1972; 260: 212
- A rapid electrophoretic technique for identification of subunit species of apoproteins in serum lipoproteins.Anal. Biochem. 1973; 53: 497
- Apoprotein composition of very low density lipoproteins of human serum.J. Clin. Invest. 1975; 56: 1622
- 3rd edition. Manual of Laboratory Operations, Lipid Research Clinics Program. Vol. 1. U.S. Government Printing Office, Washington, D.C1975 (DHEW Publication, No. (NIH) 75-628)
- Phosphorus assay in column chromatography.J. Biol. Chem. 1959; 234: 466
- Metabolism of apoprotein B of very low density lipoproteins in the rat.J. Clin. Invest. 1975; 56: 1396
- Catabolism of very low density lipoproteins in the rabbit — Effect of changing composition and pool size.Biochim. Biophys. Acta. 1978; 528: 176
- The reliability of molecular weight determinations by dodecylsulphate-polyacrylamide gel electrophoresis.J. Biol. Chem. 1969; 244: 4406
- The theory of tracer experiments with 131I-labelled plasma proteins.Phys. Med. Biol. 1957; 2: 36
- Very low density lipoproteins in normal and cholesterol fed rabbits, Part 2 (Metabolism of very low density lipoproteins in rabbits).Atherosclerosis. 1976; 23: 85
- High density lipoprotein metabolism in normolipidemic and cholesterol-fed rabbits.Biochim. Biophys. Acta. 1978; 530: 132
- Metabolism of rat plasma very low density lipoprotein, Part 1 (Fate in circulation of the whole lipoprotein).Biochim. Biophys. Acta. 1973; 326: 378
- Interaction of rat plasma very low density lipoprotein with lipoprotein-rich (postheparin) plasma.J. Lipid Res. 1975; 16: 341
- Lipid changes in plasma lipoproteins of baboons given an atherogenic diet, Part 3 (A comparison between the plasma of the baboon and chimpanzee given atherogenic diets and lipid changes in human plasma lipoproteins of type II hyperlipoproteinemia).Atherosclerosis. 1972; 16: 257
- Cholesterol feeding revised.Circulation. 1976; 54: 178
- Short term cholesterol feeding in humans — Failure to induce β-migrating very low density lipoproteins (β-VLDL).Clin. Res. 1977; 25: 158A
Article info
Publication history
Accepted:
May 24,
1978
Received in revised form:
May 22,
1978
Received:
March 29,
1978
Footnotes
☆This work has been supported by NIH grants HL-18645 and HL-11775.
Identification
Copyright
© 1978 Published by Elsevier Inc.