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Lipoprotein metabolism in baboons

Effect of feeding cholesterol-rich diet
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      Abstract

      Cholesterol-fed male baboons were selected as a model of human hyperlipidemia. Four chaired baboons fed a diet rich in cholesterol for 6 months had a 100% increase in whole plasma cholesterol, 22% increase in phospholipids with a 44% decrease in triglyceride. Total proteins of very low (VLDL) and high (HDL) density lipoproteins declined by 40% and 15% respectively. Total proteins of intermediate (LDL) and low (LDL) density lipoproteins, however, increased considerably. The cholesterol/protein and cholesterol/triglyceride ratio increased in all lipoproteins. In a separate experiment two animals were employed to study VLDL turnover. These studies were conducted once while they were on a standard diet and once after they had been on an atherogenic diet for 6 months. Radioactivity from VLDL apolipoprotein B in normolipidemic donors and recipients decayed biphasically with the immediate appearance of a large proportion of it in IDL and LDL. The first phase was much more rapid (Math Eq, 0.53 h) than the second (Math Eq, 20.5 h). Most of the VLDL apolipoprotein B (98.5%) decayed via the rapid first phase with a fractional catabolic rate (FCR) of 0.77 h−1. In cross-over studies, half-life of each phase of VLDL apolipoprotein B decay and FCR depended upon the donor and recipient dietary status. Lowest fractional catabolic rate (0.22 h−1) was observed in case of hypercholesterolemic donor and recipient.

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