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Research paper| Volume 31, ISSUE 3, P259-271, November 01, 1978

Copper deficiency and cholesterol metabolism in the rat

  • Kenneth G.D. Allen
    Footnotes
    Affiliations
    Department of Biochemistry, University of North Dakota and United States Department of Agriculture, Science and Education Administration, Human Nutrition Laboratory, Grand Forks, ND 58201 U.S.A.
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  • Leslie M. Klevay
    Affiliations
    Department of Biochemistry, University of North Dakota and United States Department of Agriculture, Science and Education Administration, Human Nutrition Laboratory, Grand Forks, ND 58201 U.S.A.
    Search for articles by this author
  • Author Footnotes
    ∗ Present address: Department of Food Science and Nutrition, Colorado State University, Fort Collins, C080523, U.S.A.
DOI:https://doi.org/10.1016/0021-9150(78)90062-X
Copper deficiency and cholesterol metabolism in the rat
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      Abstract

      The influence of copper deficiency on both 3H incorporation into plasma and liver lipids following [3H]mevalonate injection, and the excretion of biliary steroids from cannulated bile ducts was examined in the rat in two separate experiments. Copper-deficient rats exhibited a significant hypercholesterolemia (129% increase, P < 0.001) at 181 days. Four hours after [3H]mevalonate injection, 3H incorporation into plasma free and esterified cholesterol was elevated 162% (P < 0.005) and 80% (P < 0.025), respectively, and 3H incorporation into liver total lipids and cholesterol esters was depressed 18% (P < 0.04) and 43% (P < 0.005, respectively, in copper-deficient rats. Hepatic copper concentration of deficient rats showed a significant negative correlation with 3H incorporation into plasma cholesterol (r= −0.83, P < 0.02) and cholesterol ester (r = −0.70, P < 0.08). Total hepatic cholesterol remained unchanged by deficiency despite the significant increase in total plasma cholesterol burden of copper deficient rats (82%, P < 0.001). Copper deficiency failed to impair biliary steroid excretion. The 56% (P < 0.001) increase in bile acid excretion in the first 8 hours was paralleled by the 62% (P < 0.005) increase in plasma cholesterol concentration of deficient rats.
      The observations suggest a more rapid synthesis and clearance of cholesterol to the plasma pool, with this cholesterol being unavailable for excretion as biliary steroids, in copper-deficient rats.

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      Article Info

      Publication History

      Accepted: August 11, 1978
      Received in revised form: July 31, 1978
      Received: March 6, 1978

      Identification

      DOI: https://doi.org/10.1016/0021-9150(78)90062-X

      Copyright

      © 1978 Published by Elsevier Inc.

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