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Cholesterol ester exchange between human plasma high and low density lipoproteins mediated by a plasma protein factor

  • A. Sniderman
    Affiliations
    Cardiovascular Research Unit, Royal Victoria Hospital and Clinical Research Institute of Montreal, affiliated to the University of Montreal, Montreal, Que., Canada
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  • B. Teng
    Affiliations
    Cardiovascular Research Unit, Royal Victoria Hospital and Clinical Research Institute of Montreal, affiliated to the University of Montreal, Montreal, Que., Canada
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  • C. Vezina
    Affiliations
    Cardiovascular Research Unit, Royal Victoria Hospital and Clinical Research Institute of Montreal, affiliated to the University of Montreal, Montreal, Que., Canada
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  • Y.L. Marcel
    Affiliations
    Cardiovascular Research Unit, Royal Victoria Hospital and Clinical Research Institute of Montreal, affiliated to the University of Montreal, Montreal, Que., Canada
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      Abstract

      Although unesterified cholesterol and phospholipids exchange freely, a protein factor from the d > 1.25 g/ml plasma fraction was found to be necessary for cholesterol esters to transfer from HDL to LDL. This transfer was reversible, time-dependent and a function of the concentration of the d > 1.25 fraction, but independent of lecithin : cholesterol acyltransferase reaction. The transfer represented an equilibration of molecules, but no net mass transfer of cholesterol esters could be demonstrated from HDL to LDL.

      Keywords

      Abbreviations:

      HDL (high density lipoprotein), LDL (low density lipoprotein), VLDL (very low density lipoproteins), LCAT (lecithin: cholesterol acyltransferase), DTNB (dithionitrobenzoic acid), EDTA (ethylene diamine tetracetate)
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