Association of structural glycoproteins with lipids or lipoproteins

      This paper is only available as a PDF. To read, Please Download here.


      Gradient density ultracentrifugation was used to assess the lipid binding of 125I-labelled structural glycoproteins and standard proteins. The results show that solubilized structural glycoproteins from cartilage or aorta will associate spontaneously with lipids or lipoproteins. In addition, structural glycoprotein preparations from tissues which have not been delipidated contain a fraction which is already associated with lipid. Standard proteins do not behave in the same way. It is proposed that structural glycoprotein-lipid binding may be one of the mechanisms involved in the intercellular deposition of plasma lipoproteins in atherosclerotic arteries.



      LDL (low density lipoprotein), LPDS (lipoprotein-deficient serum)
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'


      Subscribe to Atherosclerosis
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


        • Barnes M.J.
        • Partridge S.M.
        The isolation and characterization of a glycoprotein from human thoracic aorta.
        Biochem. J. 1968; 109: 883
        • Ross R.
        • Bornstein P.
        The elastic fiber, Part 1 (The separation and partial characterization of its macromolecular components).
        J. Cell Biol. 1969; 40: 366
        • Robert L.
        • Moczar E.
        • Robert B.
        Biochemical and immunological properties of the structural glycoproteins of connective tissue.
        in: 4th Int. Conf, on Cystic Fibrosis of the Pancreas, Part II, Karger, Basle1968: 309-317
        • Shipp D.W.
        • Bowness J.M.
        Insoluble non-collagenous cartilage glycoproteins with aggregating subunits.
        Biochem, Biophys. Acta. 1975; 379: 282
        • Bowness J.M.
        Intercellular glycoproteins and low density lipoprotein binding to fibroblasts.
        Atherosclerosis. 1977; 27: 221
        • Moczar M.
        • Moczar E.
        • Robert L.
        Structural glycoprotein from the media of pig aorta - Aggregation of the S-carboxamidomethyl subunits.
        Biochimie. 1977; 59: 141
        • Hatch F.T.
        • Lees R.S.
        Practical methods for plasma lipoprotein analysis.
        Adv. Lipid Res. 1968; 6: 1
        • McFarlane A.S.
        Efficient trace-labelling of proteins with iodine.
        Nature (Lend.). 1958; 182: 53
        • Deckelbaum R.J.
        • Shipley G.G.
        • Small D.M.
        Structure and interactions of lipids in human plasma low density lipoproteins.
        J. Biol. Chem. 1977; 252: 744
        • Segrest J.P.
        Amphipathic helixes and plasma lipoproteins - Thermodynamic and geometric considerations.
        Chem. Phys. Lipids. 1977; 18: 7
        • Yamada K.M.
        • Schlesinger D.H.
        • Kennedy D.W.
        • Pastan L.
        Characterization of a major fibroblast cell surface glycoprotein.
        Biochemistry. 1977; 16: 5552
        • Muir L.W.
        • Bornstein P.
        • Ross R.
        A presumptive subunit of elastic fiber microfibrils secreted by arterial smooth muscle cells in culture.
        Europ. J. Biochem. 1976; 64: 105
        • Bowness J.M.
        Lipoproteins versus structural glycoproteins - Atherosclerosis as an analogue and competitor of normal connective tissue interactions.
        Med. Hypotheses. 1976; 2: 200
        • Srinivasan S.R.
        • Dolan P.
        • Radhakrishnamurthy B.
        • Berenson G.S.
        Isolation of lipoprotein-acid mucopolysaccharide complexes from fatty streaks of human aortas.
        Atherosclerosis. 1972; 16: 95
        • Hollander W.
        Role of arterial lipoproteins in the formation of the fibrous plaque.
        in: Manning G.W. Haust M.D. Atherosclerosis-Metabolic, Morphologic and Clinical Aspects. Plenum Press, New York, NY1976: 793
        • LaBella F.S.
        Elastin, a metabolically active lipoprotein.
        Nature (Lend.). 1957; 180: 1360
        • Kramsch D.M.
        • Franzblau C.
        • Hollander W.
        The protein and lipid composition of arterial elastin and its relationship to lipid accumulation in the atherosclerotic plaque.
        J. Clin. Invest. 1971; 50: 1666
        • Haust M.D.
        • More R.H.
        Mechanisms of fibrosis in white atherosclerotic plaques of human aorta - An electron microscope study.
        Circulation. 1966; 34: 14
        • Robert L.
        • Robert A.M.
        Pathophysiology of the intercellular matrix as a whole.
        in: Manning G.W. Haust M.D. Atherosclerosis-Metabolic, Morphologic and Clinical Aspects. Plenum Press, New York, NY1976: 762