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The action of human high density lipoprotein on cholesterol crystals Part 1. Light-microscopic observations

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      Abstract

      High density lipoprotein (HDL) was found in vitro to form myelin buds (liposomes) from washed crystals of free cholesterol (commercial or atheroma sources). This activity led to the progressive destruction and solubilization of the crystals. Low density and very low density lipoproteins did not have any effect. Liposome formation and solubilization were accelerated by calcium ions, phospholipase A and polyunsaturated lecithin (Lipostabil). Cholesterol crystals were nearly completely destroyed after 18 h incubation with HDL-Lipostabil.

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      References

        • Armstrong M.L.
        • Megan M.B.
        Lipid depletion in atheromatous coronary arteries in rhesus monkeys after regression diets.
        Circulat. Res. 1972; 30: 675
        • Vesselinovitch D.
        • Wissler R.W.
        • Hughes R.
        • Borensztajn J.
        Reversal of advanced atherosclerosis in rhesus monkeys, Part 1 (Light-microscopic studies).
        Atherosclerosis. 1976; 23: 155
        • Adams C.W.M.
        • Morgan R.S.
        • Bayliss O.B.
        No regression of atheroma over one year in rabbits previously fed a cholesterol-enriched diet.
        Atherosclerosis. 1973; 18: 429
        • Adams C.W.M.
        • Bayliss O.B.
        • Turner D.R.
        Phagocytes, lipid-removal and regression of atheroma.
        J. Path. 1975; 116: 225
        • Gould R.J.
        • Jones R.J.
        • Wissler R.W.
        Lability of cholesterol in human atherosclerotic plaques.
        Circulation. 1959; 20: 967
        • Field H.
        • Swell L.
        • Schools P.E.
        • Treadwell C.R.
        Dynamic aspects of cholesterol metabolism in different areas of aorta and other tissues in man and their relationships to atherosclerosis.
        Circulation. 1960; 22: 547
        • Goodman De W.S.
        • Noble R.P.
        Turnover of plasma cholesterol in man.
        J. Clin. Invest. 1968; 47: 231
        • Adams C.W.M.
        Tissues changes and lipid entry in developing atheroma.
        in: Atherogenesis: Initiating Factors, CIBA Foundation Symposium NS 12. 1973: 5-37
        • Bondjers G.
        • Björkerud S.
        Cholesterol transfer between arterial smooth muscle tissue and serum lipoproteins in vitro.
        Artery. 1974; 1: 3-9
        • Stein Y.
        • Glangeaud M.C.
        • Fainaru M.
        • Stein O.
        The removal of cholesterol ester from aortic smooth muscle cells in culture and Landschutz ascites cells by fractions of high density lipoprotein.
        Biochim. Biophys. Acta. 1975; 380: 106
        • Carew T.E.
        • Hayes S.B.
        • Koschinsky T.
        • Steinberg D.
        A mechanism by which high density lipoproteins may slow the atherogenic process.
        Lancet. 1976; 1: 1315
        • Stein O.
        • Vanderhoek J.
        • Stein Y.
        Cholesterol content and sterol synthesis in human skin fibroblasts and rat aortic smooth muscle cells exposed to lipoprotein depleted serum and high density lipoprotein/phospholipid mixtures.
        Biochim. Biophys. Acta. 1976; 431: 347
        • Leathes J.B.
        Role of fats in vital phenomena, Lecture III. Myelin forms of lecithine.
        Lancet. 1925; 1: 957
        • Bangham A.D.
        Lipid bilayers and biomembranes.
        Ann. Rev. Biochim. 1972; 41: 753
        • Burstein M.
        • Scholnick J.R.
        Lipoprotein-polyanion-metal interactions.
        Adv. Lipid Res. 1973; 11: 67
        • Fieser L.F.
        Cholesterol and comparisons, Part 7 (Steroid dibromides).
        J. Amer. Chem. Soc. 1953; 75: 5421
        • Adams C.W.M.
        Histochemical mechanism of the Marchi reaction for degenerating myelin.
        J. Neurochem. 1958; 2: 178
        • Abdulla Y.H.
        • Adams C.W.M.
        The action of human high density lipoprotein on cholesterol crystals, Part 2 (Biochemical observations).
        Atherosclerosis. 1978; 31: 473
        • Rothblat G.H.
        • Buchko M.K.
        • Kritchevsky D.
        Cholesterol uptake by L 5178 Y tissue culture cells — Studies with delipidized serum.
        Biochim. Biophys. Acta. 1968; 164: 327
        • Adams C.W.M.
        • Abdulla Y.H.
        • Bayliss O.B.
        • Morgan R.S.
        Modification of aortic atheroma and fatty liver in cholesterol-fed rabbits by intravenous injection of saturated and polyunsaturated lecithins.
        J. Path. Bact. 1967; 94: 77
        • Patelski J.
        • Bowyer D.E.
        • Howard A.N.
        • Jennings I.W.
        • Thorne C.J.R.
        • Gresham G.A.
        Modification of enzyme activities in experimental atherosclerosis in the rabbit.
        Atherosclerosis. 1970; 12: 41
        • Stein Y.
        • Stein O.
        Lipid synthesis and degradation and lipoprotein transport in mammalian aorta.
        in: Atherogenesis: Initiating Factors, CIBA Foundation Symposium NS 12. 1973: 165
        • Glomsett J.A.
        The plasma lecithin : cholesterol acyltransferase reaction.
        J. Lipid Res. 1968; 9: 155