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Isolation and characterization of the major lipoprotein density classes of normal and diabetic baboon (Papio anubis) plasma

  • Dubo Bojanovski
    Footnotes
    Affiliations
    Institut fur Klinische Biochemie and Physiologische Chemie der Medizinischen Hochschule, Hannover, G.F.R.

    Laboratory of Lipid and Lipoprotein Studies, Oklahoma Medical Research Foundation, and Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA

    Department of Pathology, The University of Texas Medical Branch, Galveston, TX 77550, U.S.A.
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  • Petar Alaupovic
    Affiliations
    Institut fur Klinische Biochemie and Physiologische Chemie der Medizinischen Hochschule, Hannover, G.F.R.

    Laboratory of Lipid and Lipoprotein Studies, Oklahoma Medical Research Foundation, and Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA

    Department of Pathology, The University of Texas Medical Branch, Galveston, TX 77550, U.S.A.
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  • Jim L. Kelley
    Affiliations
    Institut fur Klinische Biochemie and Physiologische Chemie der Medizinischen Hochschule, Hannover, G.F.R.

    Laboratory of Lipid and Lipoprotein Studies, Oklahoma Medical Research Foundation, and Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA

    Department of Pathology, The University of Texas Medical Branch, Galveston, TX 77550, U.S.A.
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  • Clarke Stout
    Affiliations
    Institut fur Klinische Biochemie and Physiologische Chemie der Medizinischen Hochschule, Hannover, G.F.R.

    Laboratory of Lipid and Lipoprotein Studies, Oklahoma Medical Research Foundation, and Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA

    Department of Pathology, The University of Texas Medical Branch, Galveston, TX 77550, U.S.A.
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  • Author Footnotes
    ∗ D. Boianovski was recipient of a NATO-Fellowship (430/402/859/4) from the Deutscher Akademischer Austauschdienst.
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      Abstract

      Lipoproteins from the plasma of normal and diabetic baboons (Papio anubis) were separated into very low density lipoproteins (VLDL, d 1.006 g/ml), low density lipoproteins (LDL, d 1.006–1.063 g/ml) and high density lipoproteins (HDL, d 1.063–1.21 g/ml) and the density classes were characterized by immunologic methods and by quantitative determination of protein and lipid. Diabetes was induced by surgical removal of the pancreas and the hyperglycemia was controlled by daily insulin injection.
      HDL were the major density class of normal baboon plasma representing 66% of the total lipoprotein content. LDL accounted for 31% and the VLDL for only 3%. Diabetes had a profound effect on the distribution of baboon lipoproteins. VLDL accounted for 37%, LDL for 35% and HDL for only 28% of the total lipoprotein content. In normal baboons, HDL was the main vehicle for the transport of phospholipids and glycerides with cholesterol almost equally distributed between HDL and LDL. In diabetic animals, free and esterified cholesterol were transported mainly by LDL and the glycerides by VLDL. Cholesterol esters accounted for approximately 75% of the total cholesterol in both the normal and diabetic baboons.
      Studies on the immunologic properties of baboon lipoproteins showed a marked heterogeneity of all 3 major density classes with respect to antigenic composition. Antisera to human apolipoproteins A (A-I and A-II), B, C (C-I, C-II and C-III), D and E cross-reacted with baboon lipoproteins suggesting a close analogy between apolipoproteins of these two species.

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      References

        • McGill Jr., H.C.
        • Strong J.C.
        • Holman R.L.
        • Werthenssen N.T.
        Arterial lesions in the Kenya baboon.
        Circulation Res. 1960; 8: 670
        • Lindsay S.
        • Chaikoff I.L.
        Naturally occurring arteriosclerosis in nonhuman primates.
        J. Atheroscler. Res. 1966; 6: 36
        • Howard A.N.
        • Gresham G.A.
        • Bowyer D.E.
        • Lindgren F.T.
        Aortic and coronary atherosclerosis in baboons.
        Progr. Biochem. Pharmacol. 1968; 4: 438
        • Strong J.P.
        • McGill Jr., H.C.
        Diet and experimental atherosclerosis in baboons.
        Amer. J. Path. 1967; 50: 669
        • Blaton V.
        • Howard A.N.
        • Gresham G.A.
        • Vandamine D.
        • Peeters H.
        Lipid changes in the plasma lipoproteins of baboons given an atherogenic diet, Part 1 (Changes in the lipids of total plasma and wand gb-lipoproteins).
        Atherosclerosis. 1970; 11: 497
        • Eggen D.A.
        • Strong J.P.
        Diet and cholesterol metabolism in young and old baboons.
        Atherosclerosis. 1970; 12: 359
        • Kotze J.P.
        • Kempff P.G.
        Nutritional regimens and their effects on the lipid metabolism of the baboon (Papio ursinus).
        S. Afr. J. Sci. 1973; 69: 371
        • McGill Jr., H.C.
        • Strong J.P.
        • Newman W.P.
        • Eggen D.A.
        The baboon in atherosclerosis research.
        in: The Baboon in Medical Research. Vol. 11. University of Texas Press, Austin, TX1967: 351
        • Shapiro I.L.
        • Jastremsky J.A.
        • Kritchevsky D.
        • Eggen D.A.
        Transport of cholesterol in the baboon.
        in: The Baboon in Medical Research. Vol. 11. University of Texas Press, Austin, TX1967: 571
        • Kelley J.L.
        • Alaupovic P.
        Lipid transport in the avian species, Part 1 (Isolation and characterization of apolipoproteins and major lipoprotein density classes of male turkey serum).
        Atherosclerosis. 1976; 24: 155
        • Ouchterlony Ö.
        Antigen-antibody reactions in gels, Part 4 (Types of reactions in coordinated systems of diffusion).
        Acta Path. Microbiol. 1953; 32: 231
        • Alaupovic P.
        • Lee D.M.
        • McConathy W.J.
        Studies on the composition and structure of plasma lipoproteins — Distribution of lipoprotein families in major density classes of normal human plasma lipoproteins.
        Biochem. Biophys. Acta. 1972; 260: 689
        • Kostner G.
        • Alaupovic P.
        Studies of the composition and structure of plasma lipoproteins — Separation and quantification of the lipoprotein families occurring in the high density lipoproteins of human plasma.
        Biochemistry. 1972; 11: 3419
        • McConathy W.J.
        • Alaupovic P.
        Studies on the isolation and partial characterization of apolipoprotein D and lipoprotein D of human plasma.
        Biochemistry. 1976; 15: 515
        • Curry M.D.
        • McConathy W.J.
        • Alaupovic P.
        • Ledford J.H.
        • Popović M.
        Determination of human apolipoprotein E by electroimmunoassay.
        Biochim. Biophys. Acta. 1976; 439: 413
        • Mills G.L.
        • Taylour C.E.
        The distribution and composition of serum lipoproteins in eighteen animals.
        Comp. Biochem. Physiol. 1971; 40-B: 489
        • Peeters H.
        • Blaton V.
        • Declercq B.
        • Howard A.N.
        • Gresham G.A.
        Lipid changes in the plasma lipoproteins of baboons given an atherogenic diet, Part 2 (Changes in the phospholipid classes of total plasma and of α- and (β-lipoproteins).
        Atherosclerosis. 1970; 12: 283
        • Srinivasan S.R.
        • McBride Jr., J.R.
        • Radhakrishnamurthy B.
        • Berenson G.S.
        Comparative studies on serum lipoprotein and lipid profiles in subhuman primates.
        Comp. Biochem. Physiol. 1974; 47-B: 711
        • Alaupovic P.
        Conceptual development of the classification systems of plasma lipoproteins.
        in: Peeters H. Protides of the Biological Fluids. Proc. Colloq.Vol. 19. Pergamon Press, London1972: 9
        • Mahley R.W.
        • Weisgraber K.H.
        • Innerarity T.
        • Brewer Jr., H.B.
        Characterization of the plasma lipoproteins and apoproteins of the Erythrocebus patas monkey.
        Biochemistry. 1976; 15: 1928
        • Scanu A.M.
        • Edelstein C.
        • Vitello L.
        • Jones R.
        • Wissler R.
        The serum high density lipoproteins of Macacus rhesus, Part 1 (Isolation, composition and properties).
        J. Biol. Chem. 1973; 248: 7648
        • Blaton V.
        • Vercaemst R.
        • Vandecasteele N.
        • Caster H.
        • Peeters H.
        Isolation and partial characterization of chimpanzee plasma high density lipoproteins and their apolipoproteins.
        Biochemistry. 1974; 13: 1127
        • Lee J.A.
        • Morris M.D.
        Characterization of the serum low density lipoproteins of normal on two Rhesus monkeys with spontaneous hyperbeta-lipoproteinemia.
        Biochem. Med. 1974; 10: 245
        • Hamwi G.J.
        • Garcia O.
        • Kruger F.A.
        • Gwinup G.
        • Cornwell D.G.
        Hyperlipidemia in uncontrolled diabetes.
        Metabolism. 1972; 11: 850