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Abstract
Specific areas of fourteen autopsied abdominal aortas were layer-dissected, histologically
graded and solubilized with SDS, dilute saline or SDS-urea and β-mercaptoethanol.
Comparisons were made between intima, media, lesions of progressive severity and an
in-vivo thrombus. Apparent molecular weights were calculated from the Coomassie blue
stained gels of these extracts and each band's contribution estimated by integrative
densitometry. Four minor high molecular weight bands and six medium to low molecular
weight bands were detected in the arterial extracts. Band intensity stain patterns
of normal intima are easily distinguished from normal media. Extracts of minor lesions
resemble normal media; higher grade lesions demonstrate increased amounts of characteristic
intimal bands. The major medial band is also seen on gels of thrombus extracts. Both
of the bands most characteristic of atherosclerotic lesions stain for carbohydrate.
Isolation and characterization of these (glyco)proteins will provide material for
binding studies. Quantitation of characteristic lesion proteins may provide insights
into the proliferative phase of this disease.
Keywords
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Article info
Publication history
Accepted:
June 26,
1978
Received in revised form:
June 22,
1978
Received:
February 21,
1978
Footnotes
☆This study was supported in part by National Institutes of Health grant No. S R01 HL20471.
Identification
Copyright
© 1978 Published by Elsevier Inc.
