Glycosaminoglycan—Lipoprotein complexes from aortas of hypercholesterolemic rabbits

Part 1. Isolation and characterization
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      Glycosaminoglycan—lipoprotein complexes were isolated from rabbit aortas exhibiting nearly confluent cholesterol-induced foam cell lesions by extraction with 0.15 M NaCl. Purification and characterization was achieved by gel chromatography, non-ionic differential flotation and by cellulose polyacetate electrophoresis. Analysis showed that these complexes consisted of very low density lipoproteins, heparan sulfate, chondroitin sulfate-C and hyaluronic acid. The demonstration that rabbit intimal foam cell lesions contain extractable glycosaminoglycan—lipoprotein complexes makes this animal model an excellent tool for further studies on the role of these complexes in the atherogenic process.


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