Advertisement

Glycosaminoglycan—Lipoprotein complexes from aortas of hypercholesterolemic rabbits

Part 1. Isolation and characterization
      This paper is only available as a PDF. To read, Please Download here.

      Abstract

      Glycosaminoglycan—lipoprotein complexes were isolated from rabbit aortas exhibiting nearly confluent cholesterol-induced foam cell lesions by extraction with 0.15 M NaCl. Purification and characterization was achieved by gel chromatography, non-ionic differential flotation and by cellulose polyacetate electrophoresis. Analysis showed that these complexes consisted of very low density lipoproteins, heparan sulfate, chondroitin sulfate-C and hyaluronic acid. The demonstration that rabbit intimal foam cell lesions contain extractable glycosaminoglycan—lipoprotein complexes makes this animal model an excellent tool for further studies on the role of these complexes in the atherogenic process.

      Keywords

      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Atherosclerosis
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

        • Iverius P.H.
        Porter R. Knight J. Atherogenesis: Initiating Factors. Elsevier, Amsterdam1973: 185-196
        • Iverius P.H.
        The interaction between human plasma lypoproteins and connective tissue glycosaminoglycans.
        J. Biol. Chem. 1965; 247: 2607
        • Hollander W.
        Unified concept on the role of acid mucopolysaccharides and connective tissue proteins in the accumulation of lipids, lipoproteins and calcium in the atherosclerotic plaque.
        Exp. Mol. Path. 1976; 25: 106
        • Bihari-Varga M.
        Precipitation complexes formed from mucopolysaccharides and serum β-lipoprotein with the introduction of metal ions.
        Acta Chim. Acad. Sci. Hung. 1965; 45: 219
        • Srinivasan S.R.
        • Radhakrishnamurthy B.
        • Berenson G.S.
        Studies on the interaction of heparin with serum lipoproteins in the presence of Ca2+, Mg2+ and Mn2+.
        Arch. Biochem. Biophys. 1975; 170: 334
        • Burstein M.
        • Scholnick H.R.
        • Morfin R.
        Rapid method for the isolation of lipoproteins from human serum by precipitation with polyanions.
        J. Lipid Res. 1970; 11: 583
        • Jordan Jr., W.J.
        • Faulkner A.G.
        • Knoblock E.C.
        Heparin-calcium complexes as a measure of serum low density lipoproteins.
        Anal. Biochem. 1966; 14: 91
        • Bihari-Varga M.
        • Vegh M.
        Quantitative studies on the complexes formed between aortic mucopolysaccharides and serum lipoproteins.
        Biochem. Biophys. Acta. 1967; 144: 202
        • Amenta J.S.
        • Waters L.L.
        The precipitation of serum lipoproteins by mucopolysaccharides extracted from aortic tissue.
        Yale J. Biol. Med. 1960; 33: 112
        • Gero S.
        • Gergely J.
        • Déveńyi T.
        • Jakab L.
        • Székely J.
        • Virág S.
        Role of intimal mucoid substances in the pathogenesis of atherosclerosis, Part 1 (Complex formation in vitro between mucopolysaccharides from atherosclerotic aortic intimas and plasma β-lipoprotein and fibrinogen).
        J. Atheroscler. Res. 1961; 1: 67
        • Gerö S.
        • Gergely J.
        • Déveńyi T.
        • Jakab L.
        • Székely J.
        • Virág S.
        Role of mucoid substances of the aorta in the deposition of lipids.
        Nature (Lond.). 1960; 187: 152
        • Bihari-Varga M.
        • Gergely J.
        • Gerö S.
        Further investigations on complex formation in vitro between aortic mucopolysaccharides and β-lipoproteins.
        J. Atheroscler. Res. 1964; 4: 106
        • Haust M.D.
        Electronmicroscopic and immunohistochemical studies of fatty streaks in human aorta.
        Progr. Biochem. Pharmacol. 1968; 4: 429
        • Kao V.C.
        • Wissler R.W.
        A study of the immunohistochemical localization of serum lipoproteins and other plasma proteins in human atherosclerotic lesions.
        Exp. Mol. Path. 1965; 4: 465
        • Watt H.E.
        Jones R.J. Evolution of the Atherosclerotic Plaque. University of Chicago Press, Chicago, IL1964: 117-132
        • Walton K.W.
        • Williamson N.
        Histological and immunofluorescent studies on the human atheromatous plaque.
        J. Atheroscler. Res. 1968; 8: 599
        • Walton K.W.
        Bittar E.E. Bittar N. Biological Basis of Medicine. Academic Press, New York, NY1969: 193-233
        • Hanig M.
        • Shainoff J.R.
        • Lowry A.D.
        Flotational lipoproteins extracted from human atherosclerotic aortas.
        Science. 1956; 124: 176
        • Hoff H.F.
        • Heidemann C.L.
        • Jackson R.L.
        The localization patterns of plasma apoproteins in human lesions.
        Circ. Res. 1975; 37: 72
        • Woolf N.
        • Crawford T.
        Fatty streaks in the aortic intima studied by immunohistochemical techniques.
        J. Path. 1960; 80: 405
        • Hoff H.F.
        • Jackson R.L.
        • Mao S.J.T.
        • Gotto Jr., A.M.
        Localization of low density lipoproteins in atherosclerotic lesions from human normolipemics employing a purified fluorescent labeled antibody.
        Biochim. Biophys. Acts. 1974; 351: 407
        • Hoff H.F.
        • Lie J.T.
        • Titus J.L.
        Lipoproteins in atherosclerotic lesions. Localization by immunofluorescence of apo-low density lipoproteins in human atherosclerotic arteries from normals and hyperlipoproteinemics.
        Arch. Path. 1975; 99: 253
        • Woolf N.
        • Pilkington T.R.
        Immunohistochemical demonstration of lipoproteins in vessel walls.
        J. Path. 1963; 90: 459
        • Choi J.H.
        Demonstration of lipoproteins in human atherosclerotic lesions by the fluorescent antibody technique.
        in: Fed. Proc.25. 1966: 665
        • Srinivasan S.R.
        • Dolan P.
        • Radhakrishnamurthy B.
        • Pargaonkar P.S.
        • Berenson G.S.
        Isolation of lipoprotein acid mucopolysaccharide complexes from fatty streaks of human aortas.
        Atherosclerosis. 1972; 16: 95
        • Srinivasan S.R.
        • Dolan P.
        • Radhakrishnamurthy B.
        • Pargaonkar P.S.
        • Berenson G.S.
        Lipoprotein acid mucopolysaccharide complexes of human atherosclerotic lesions.
        Biochim. Biophys. Acta. 1975; 388: 58
        • Fu S.T.
        • Ho K.J.
        • Taylor C.B.
        • Manalo-Estrella P.
        Cholesterol and acid mucopolysaccharides in hypercholesterolemic rabbits.
        Arch. Path. 1972; 94: 466
        • Virág S.
        • Denes R.
        • Pozsonyi T.
        Uptake of [125I]β-lipoprotein by aortas and veins of normal and cholesterol fed rabbits.
        Atherosclerosis. 1970; 11: 361
        • Christensen S.
        Intimal uptake of plasma lipoprotein and atherosclerosis.
        Progr. Biochem. Pharmacol. 1967; 4: 244
        • Virág S.
        • Denes R.
        • Pozsonyi T.
        Relationship between concentration of acid mucopolysaccharides in the aortic wall and degree of incorporation of β-lipoproteins.
        Byullenten' Eksperimentel'-noi Biologii i Meditsiny. 1970; 70: 37
        • Roden L.
        • Baker J.R.
        • Cifonelli J.A.
        • Mathews M.B.
        Methods in Enzymology. Vol. 28. Academic Press, New York, NY1972: 73-104
        • Huang T.C.
        • Chen C.T.
        • Weffler V.
        • Raftery A.
        A stable reagent for the Liebermann—Burchard reaction — Application to rapid serum cholesterol determination.
        Anal. Chem. 1961; 33: 1405
        • Wardi A.H.
        • Allen W.S.
        • Verma R.
        A simple method for the detection and quantitative determination of hexuronic acids and pentoses.
        Anal. Biochem. 1974; 57: 268
        • Lowry O.H.
        • Rosebrough N.J.
        • Farr A.L.
        • Randall R.J.
        Protein measurement with the Folin phenol reagent.
        J. Biol. Chem. 1951; 193: 265
        • Antonopoulos C.A.
        • Gardell S.
        • Hamnstrom B.
        Separation of the glycosaminoglycans (mucopolysaccharides) from aorta by a column procedure using quaternary ammonium compounds.
        J. Atheroscler. Res. 1965; 5: 9
        • Antonopoulos C.A.
        • Gardell S.
        • Szirmai J.A.
        • de Tyssonsk E.R.
        Determination of glycosaminoglycans (mucopolysaccharides) from tissues on the microgram scale.
        Biochim. Biophys. Acts. 1964; 83: 1
        • Thunell S.
        • Antonopoulos C.A.
        • Gardell S.
        Analysis of aortic glycosaminoglycans from various animal species by CPC-cellulose column procedures.
        J. Atheroscler. Res. 1967; 7: 283
        • Engel U.R.
        Glycosaminoglycans in the aorta of six animal species.
        Atherosclerosis. 1971; 13: 45
        • Ceriotti G.
        A microchemical determination of deoxyribonucleic acid.
        J. Biol. Chem. 1952; 198: 297
        • Izuka K.
        • Murata K.
        Inhibitory effects of human aortic and venous acid glycosaminoglycans on thrombus formation.
        Atherosclerosis. 1972; 16: 217
        • Murata K.
        • Oshima Y.
        Chondroitin sulfates in atherosclerotic human aorta.
        Atherosclerosis. 1971; 14: 121
        • Tracy R.E.
        • Dzoga K.R.
        • Wissler R.W.
        Sequestration of serum low density lipoproteins in the arterial intima by complex formation.
        in: Proc. Soc. Exp. Biol. Med.118. 1965: 1095
        • Sve]car J.
        • Robertson W.V.B.
        Micro separation and determination of mammalian acidic glycosaminoglycans (mucopolysaccharides).
        Anal. Biochem. 1967; 18: 333
        • Hata R.
        • Nagai Y.
        A rapid and micro method for the separation of acidic glyeosaminoglycans by two-dimensional electrophoresis.
        Anal. Biochem. 1972; 45: 462
        • Morin R.J.
        Biosynthesis of acid mucopolysaccharides by rabbit and human arteries.
        Metabolism. 1967; 16: 949
        • Ho K.J.
        • Taylor C.B.
        Mode of cholesterol accumulation in various tissues of rabbits with moderate hypercholesterolemia.
        in: Proc. Soc. Exp. Biol. Med.136. 1971: 249
        • Dalferes Jr., E.R.
        • Ruiz H.
        • Kumar V.
        • Radhakrishnamurthy B.
        • Berenson G.S.
        Acid mucopolysaccharides of fatty streaks in young, human male aortas.
        Atherosclerosis. 1971; 13: 121
        • Srinivasan S.R.
        • Lopez- S.A.
        • Radhakrishnamurthy B.
        • Berenson G.S.
        Complexing of serum pre-β- and β-lipoproteins and acid mucopolysaccharides.
        Atherosclerosis. 1970; 12: 321
        • Rodriguez J.L.
        • Catapano A.
        • Ghiselli G.C.
        • Sirtori C.R.
        Very low density lipoproteins in normal and cholesterol fed rabbits — Lipid and protein composition and metabolism.
        Atherosclerosis. 1976; 23: 85
        • Fraser R.
        • Vesselinovitch D.
        • Wissler R.W.
        New interpretations of lipoproteins and arterial lesions of cholesterol-fed rabbits.
        Pathology. 1976; 8: 57