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Endothelial morphology and plasma total and high density lipoprotein cholesterol changes in hypothalamically stimulated squirrel monkeys fed a modified atherogenic diet

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      Abstract

      Experimental animals fed atherogenic diets show endothelial damage, impairment of endothelial regeneration and plasma lipid changes characterized by elevation of LDL and decrease of HDL cholesterol concentrations. Previous studies in this laboratory disclosed that chronic electrical stimulation of the lateral hypothalamus was associated with electron-microscopic evidence of endothelial injury in rats and squirrel monkeys maintained on basal (low fat/cholesterol-free) diets. In the present investigation squirrel monkeys fed similar diets supplemented with “modest” amounts of caloric fat and cholesterol were subjected to chronic lateral hypothalamic stimulation for periods as long as 20 months with the expectation that endothelial injury would be greater than in the absence of the supplements. These expectations were not substantiated. Endothelium was found to be surprisingly intact by electron microscopy and similar to that of implanted nonstimulated controls. A further observation of interest was the cholesterolemic response, notably in the HDL fraction, observed in both groups, but more striking in experimental animals. The data suggest that an interaction between a modified lipid/cholesterol diet and hypothalamic stimulation may lead to elevation of plasma HDL cholesterol concentration and preservation of endothelial integrity. Further investigation is required to determine whether these two events are causally related.

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      References

        • Gutstein W.H.
        • Anversa P.
        • Turi G.K.
        • Korcek L.
        • Harrison J.E.
        • Kiu G.
        Effect of hypothalamic stimulation on the endothelial morphology of the aorta in the conscious squirrel monkey.
        Atherosclerosis. 1981; 39: 329
        • Gutstein W.H.
        • D'Aguillo A.F.
        • Parl F.
        • Kiu G.
        Influence of hypothalamic stimulation on large endothelial space formation in the coronary artery of the rat.
        Artery. 1975; 1: 385
        • Gutstein W.H.
        • Harrison J.
        • Parl F.
        • Kiu G.
        • Avitable M.
        Neural factors contribute to atherogenesis.
        Science. 1978; 199: 449
        • Thorgeirsson G.
        • Robertson A.L.
        The vascular endothelium-pathobiologic significance — A review.
        Amer. J. Path. 1978; 93: 803
        • Gimbrone Jr., M.A.
        Endothelial dysfunction and the pathogenesis of atherosclerosis.
        in: Gotto Jr., A.M. Smith L.C. Allen B. Atherosclerosis-V (Proc. 5th Internat. Symp.). Springer-Verlag, New York, NY1980: 415
        • Ross R.
        The arterial wall and atherosclerosis.
        Ann. Rev. Med. 1979; 30: 1
        • Ross R.
        • Harker L.
        Hyperlipidemia and atherosclerosis — Chronic hyperlipidemia initiates and maintains lesions by endothelial cell desquamation and lipid accumulation.
        Science. 1976; 193: 1094
        • Harrison J.E.
        • Avitable M.J.
        • Gutstein W.H.
        A self-powered preprogrammable, constant current electronic stimulator for implantation in conscious and unrestrained small animals.
        Electroenceph. Clin. Neurophysiol. 1978; 44: 405
        • Allain C.C.
        • Poon L.S.
        • Chan C.S.G.
        • Richmond W.
        • Fu P.C.
        Enzymatic determination of total serum cholesterol.
        Clin. Chem. 1974; 20: 470
        • Lopes-Virella M.F.
        • Stone P.
        • Ellis S.
        • Colwell J.A.
        Cholesterol determination in high density lipoproteins separated by three different methods.
        Clin. Chem. 1977; 23: 882
        • Illingworth D.R.
        Metabolism of lipoproteins in nonhuman primates — Studies on the origin of low density lipoprotein apoprotein in the plasma of the squirrel monkey.
        Biochim. Biophys. Acta. 1975; 388: 38
        • Siegel S.
        Nonparametric Statistics for the Behavioral Sciences.
        in: McGraw-Hill Kogakusha, Ltd, Tokyo1953: 89
        • Karnovsky M.J.
        A form aldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy.
        J. Cell Biol. 1965; 27: 137A
        • Gutstein W.H.
        • Parl F.
        Ultrastructural changes of coronary artery endothelium associated with biliary obstruction in the rat.
        Amer. J. Path. 1973; 71: 49
        • Srinivasan S.R.
        • McBride J.R.
        • Radhakrishnamurthy B.
        • Berenson G.S.
        Comparative studies on serum lipoprotein and lipid profiles in subhuman primates.
        Comp. Biochem. Physiol. 1974; 47: 711
        • Rudel L.L.
        • Lofland H.B.
        Circulating lipoproteins in nonhuman primates.
        in: 6th edition. Primates in Medicine. Vol. 9. S. Karger, Basel1976: 224
        • Weibel E.R.
        • Palade G.E.
        New cytoplasmic components in arterial endothelia.
        J. Cell Biol. 1964; 23: 101
        • Inoue K.
        • Ban T.
        • Kurotsu T.
        Experimental studies on the influence of the hypothalamus upon the blood lipids.
        Med. J. Osaka Univ. 1954; 5: 475
        • Gunn C.
        • Friedman G.M.
        • Byers S.O.
        Effect of chronic hypothalamic stimulation upon cholesterol-induced atherosclerosis in the rabbit.
        J. Clin. Invest. 1960; 39: 1963
        • Gutstein W.H.
        • Schneck D.J.
        • Appleton H.
        Association of increased plasma lipid levels with brain stimulation.
        Metabolism. 1968; 17: 535
        • Jastrebcova N.L.
        • Simutenko L.V.
        Role of negative and positive emotional states in variations of blood cholesterol and the level of arterial pressure.
        Dokl. Akad. Nauk. SSSR. 1971; 201: 1001
        • Arees E.A.
        • Mayer J.
        Anatomical connections between medial and lateral regions of the hypothalamus concerned with food intake.
        Science. 1967; 157: 1574
        • Fidge N.H.
        • Foxman C.J.
        In vivo transformation of rat plasma very low density lipoprotein into higher density lipoproteins.
        Austr. J. Exp. Biol. Med. Sci. 1971; 49: 581
        • Illingworth D.R.
        • Portman O.W.
        Exchange of phospholipids between low and high density lipoproteins of squirrel monkeys.
        J. Lipid Res. 1972; 13: 220
        • Havel J.E.
        High-density lipoproteins, cholesterol transport and coronary heart disease.
        Circulation. 1979; 60: 1
        • Calandra S.
        • Montaguti M.
        • Cartoni V.
        • Pasquali-Ronchetti I.
        Plasma lipoproteins in rats with experimental biliary obstruction, Part 1 (A chemical study).
        Biochim. Biophys. Acta. 1975; 409: 1
        • Gutstein W.H.
        • Schneck D.J.
        • Appleton H.
        Mechanism of plasma lipid increases following brain stimulation.
        Metabolism. 1969; 18: 300
        • Wissler R.W.
        • Vesselinovitch D.
        • Getz G.S.
        Abnormalities of the arterial wall and its metabolism in atherogenesis.
        Progr. Cardiovasc. Dis. 1976; 18: 341
        • Constantinides P.
        Experimental Atherosclerosis.
        in: Elsevier, New York, NY1965: 51
        • Majno G.
        • Shea S.M.
        • Leventhal M.
        Endothelial contraction induced by histamine-type mediators — An electron microscopic study.
        J. Cell Biol. 1969; 42: 647
        • Becker C.G.
        • Nachman R.
        Contractile proteins of endothelial cells, platelets and smooth muscle.
        Amer. J. Path. 1973; 71: 1
        • Trillo A.A.
        • Prichard R.W.
        Early endothelial changes in experimental primate atherosclerosis.
        Lab. Invest. 1979; 41: 294