Advertisement

Conformational transitions in serum high density apoproteins of hypercholesterolemic monkeys

      This paper is only available as a PDF. To read, Please Download here.

      Abstract

      Conformational transitions in serum high density apolipoproteins of normal and hypercholesterolemic monkeys have been studied by circular dichroism. This study has revealed that under hypercholesterolemic conditions the secondary structure of apolipoproteins suffers permanent changes which could be observed even after a lengthy procedure of isolation, purification and delipidation of high density lipoprotein.

      Keywords

      Abbreviations:

      HDL, high density lipoproteins (), LDL, low density lipoproteins (), VLDL, very low density lipoproteins (), CD, circular dichroism ()
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Atherosclerosis
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

        • Slack J.
        Risks of ischaemic heart-disease in familial hyperlipoproteinaemic states.
        Lancet. 1969; ii: 1380
        • Cameo G.
        • Bosch V.
        • Arreaza C.
        • Mendex H.C.
        Early changes in plasma lipoprotein structure and biosynthesis in cholesterol fed rabbits.
        J. Lipid Res. 1973; 14: 61
        • Scanu A.
        Factors affecting lipoprotein metabolism.
        Adv. Lipid Res. 1965; 3: 63
        • Burstein M.
        • Scholnick H.L.
        • Morfin R.
        Rapid method for the isolation of lipoproteins from human serum by precipitation with polyanions.
        J. Lipid Res. 1970; 11: 583
        • Shore V.
        • Shore B.
        Some physical and chemical studies on the protein moiety of a high-density (1.126–1.195 g/ml) lipoprotein fraction of human serum.
        Biochemistry. 1967; 6: 1962
        • Chen Y.H.
        • Yang J.T.
        • Martinez H.M.
        Determination of the secondary structures of proteins by circular dichroism and optical rotatory dispersion.
        Biochemistry. 1972; 11: 4120
        • Scanu A.M.
        Environmental effects on the structure of human serum high density lipoprotein and its apoprotein.
        in: Proceedings of the 3rd International Symposium on Drugs Affecting Lipid Metabolism. Plenum Press, New York, NY1969: 345
        • Segrest J.P.
        Molecular packing of high density lipoproteins — A postulated functional role.
        FEBS Lett. 1979; 69: 111
        • Segrest J.P.
        • Feldman R.J.
        Amphipathic helixes and plasma lipoproteins — A computer study.
        Biopolymers. 1977; 16: 2053
        • Brown M.S.
        • Goldstein J.L.
        Receptor-mediated control of cholesterol metabolism.
        Science. 1976; 191: 150
      1. Singh, S., Unpublished observations.