Content and distribution of collagen in venous and polyester aortal grafts

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      The studies were carried out on autogenous venous and polyester grafts implanted in the abdominal aorta of dogs. These grafts were excised 1, 4, and 36 months after implantation and together with the normal arteries and veins, after dissection into intimal, medial, and adventitial layers, tested for their hydroxyproline and nitrogen content.
      It was found that the collagen content and the hydroxyproline/nitrogen ratio in the various layers of the venous and polyester grafts increased as time elapsed after the operation. The increase in collagen content was, however, much higher in the polyester grafts than in the venous.
      The relative index of the collagen content expressed by the above ratio was found to be almost equal in the intimal and medial layers of the venous and polyester grafts 3 years after the operation and to be slightly higher than the content in the corresponding layers of the normal arteries and veins.


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        • Levene C.J.
        • Poole J.C.F.
        The collagen content of normal and atherosclerotic human aortic intima.
        Brit. J. Exp. Path. 1962; 43: 469
        • McCullagh K.G.
        • Ehrhart L.A.
        Increased arterial collagen synthesis in experimental canine atherosclerosis.
        Atherosclerosis. 1974; 19: 13
        • Langner R.O.
        • Modrak J.B.
        Collagen metabolism during the early stages of cholesterol-induced atherogenesis in rabbits.
        Blood Vessels. 1976; 13: 257
        • Fischer G.M.
        • Swain M.L.
        • Cherian K.
        Increased vascular collagen and elastin synthesis in experimental atherosclerosis in the rabbit.
        Atherosclerosis. 1980; 35: 11
        • Pietilä K.
        • Nikkari T.
        Enhanced synthesis of collagen and total protein by smooth muscle cells from atherosclerotic rabbit aortas in culture.
        Atherosclerosis. 1980; 37: 11
        • Mason R.G.
        • Saba H.I.
        Normal and abnormal hemostasis — An integrated view.
        Am. J. Path. 1978; 92: 775
        • Santoro S.A.
        • Cunningham L.W.
        Collagen-mediated platelet aggregation — The role of multiple interactions between the platelet surface and collagen.
        Thrombos. Haemostas. 1980; 43: 158
        • Niewiarowski S.
        • Bańkowski E.
        • Rogowicka I.
        Studies on adsorption and activation of the Hageman factor (factor XII) by collagen and elastin.
        Thrombos. Diathes. Haemorrh. 1965; 14: 387
        • Chidi C.C.
        • Klein L.
        • DePalma R.G.
        Effect of regenerated endothelium on collagen content in the injured artery.
        Surg. Gynecol. Obstet. 1979; 148: 839
        • Iwatsuki K.
        • Cardinale G.J.
        • Spector S.
        • Udenfriend S.
        Hypertension — Increase of collagen biosynthesis in arteries but not in veins.
        Science. 1977; 198: 403
        • De Bakey M.E.
        • Jordan Jr., G.I.
        • Abbott J.P.
        • Halpert B.
        • O'Neal R.M.
        The fate of Dacron vascular grafts.
        Arch. Surg. 1964; 89: 757
        • Beebe H.G.
        • Clark W.F.
        • DeWeese J.A.
        Atherosclerotic change occurring in an autogenous venous arterial graft.
        Arch. Surg. 1970; 101: 85
        • Scott Jr., H.W.
        • Morgan C.V.
        • Bolasny B.L.
        • Lanier V.C.
        • Younger R.K.
        • Butts W.
        Experimental atherosclerosis in autogenous venous grafts.
        Arch. Surg. 1970; 101: 677
        • Prockop D.
        • Udenfriend S.
        A specific method for the analysis of hydroxyproline in tissues and urine.
        Anal. Biochem. 1960; 1: 228
        • Levison S.A.
        • Mac Fate R.P.
        Clinical Laboratory Diagnosis.
        in: Lea and Febiger, Philadelphia, PA1961: 443-444
        • Banga I.
        Structure and Function of Elastin and Collagen.
        in: Akademial Kiado, Budapest1966: 8
        • Banga I.
        Structure and Function of Elastin and Collagen.
        in: Akademial Kiado, Budapest1966: 107
        • Yonemasu K.
        • Stroud R.M.
        • Niedermeier W.
        • Butler W.T.
        Chemical studies on C1q, a modulator of immunoglobulin biology.
        Biochem. Biophys. Res. Commun. 1971; 43: 1388
        • Rauterberg J.
        • Bassewitz D.B.
        Electron microscopic investigations of type III collagen.
        HoppeSeyler's Z. Physiol. Chem. 1975; 356: 95
        • McCullagh K.A.
        • Balian G.
        Collagen characterisation and cell transformation in human atherosclerosis.
        Nature (Lond.). 1975; 258: 73
        • Madri J.A.
        • Dreyer M.S.
        • Pitlick F.A.
        • Furthmayr H.
        The collagenous components of the subendothelium.
        Lab. Invest. 1980; 43: 303
        • Jones M.
        • Conkle D.M.
        • Ferrans V.J.
        • Roberts W.C.
        • Levine F.H.
        • Melvin D.B.
        • Stinson E.B.
        Lesions observed in arterial autogenous vein grafts.
        Circulation. 1973; 48: 198
        • Glowifiski S.
        • Worowski K.
        Cathepsin D activity of venous and polyester vascular grafts implanted in the abdominal aorta of the dog.
        Atherosclerosis. 1979; 32: 43
        • Bawina M.V.
        • Kritsman M.G.
        Changes in the activity of proteolytic enzymes in the aorta in experimental atherosclerosis (in Russian).
        Biokhimiya. 1953; 18: 548
        • Kirk J.E.
        The cathepsin activity of arterial tissue in individuals of various ages.
        J. Gerontol. 1962; 17: 158
        • Scott P.G.
        • Pearson C.H.
        Cathepsin D — Cleavage of soluble collagen and crosslinked peptides.
        FEBS Letters. 1978; 88: 41
        • Balleisen L.
        • Gay S.
        • Marx R.
        • Kühn K.
        Comparative investigation on the influence of human bovine collagen types I,II,III on the aggregation of human platelets.
        Klin. Wschr. 1975; 53: 903