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Fatty acid and glucose incorporation into human adipose tissue in non-insulin-dependent diabetes and in insulinoma

Inverse relations with plasma triglyceride and glucose concentrations
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      Abstract

      Decreased fatty acid and glucose incorporation into human adipose tissue (FIAT and GLIAT) are frequently found in primary hypertriglyceridemia (HTG) and might also contribute to the defective removal of lipoprotein triglyceride (TG) in non-insulin-dependent diabetes mellitus (NIDDM).
      To study this possible mechanism, FIAT and GLIAT were determined in needle biopsy specimens from 14 patients with newly diagnosed NIDDM and in 14 age- and weight-matched controls. A patient with insulinoma and hyperinsulinism was also studied.
      FIAT and GLIAT processes were markedly reduced in patients with NIDDM that developed at the onset of maturity. Insulinoma patients, with normal plasma TG, showed FIAT-GLIAT values in the high to normal range before operation.
      A direct, highly significant correlation (P < 0.001) was demonstrated between FIAT and GLIAT in diabetics, insulinoma and controls when considered together.
      Plasma TG and glucose concentrations were inversely related to FIAT and GLIAT. These relationships were independent of the degree of obesity.
      It is suggested that impaired FIAT and GLIAT might contribute to defective TG removal and HTG which are often demonstrated in NIDDM.

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      References

        • Albrink M.J.
        • Lavietes P.H.
        • Man E.B.
        Vascular disease and serum lipids in diabetes mellitus.
        Ann. Intern. Med. 1963; 58: 305
        • Carlson L.A.
        Serum lipids in men with myocardial infarction.
        Acta Med. Scand. 1960; 167: 399
        • Greenhalgh R.M.
        • Lewis B.
        • Rosengarten D.S.
        • Calnan J.A.
        • Mervant I.
        • Martin P.
        Serum lipids and lipoprotein in peripheral vascular disease.
        Lancet. 1971; ii: 947
        • Carlson L.A.
        • Böttiger L.E.
        Ischaemic heart disease in relation to fasting values of plasma triglycerides and cholesterol—The Stockholm Prospective Study.
        Lancet. 1972; i: 865
        • Olsson A.G.
        Studies in asymptomatic primary hyperlipidemia.
        Acta Med. Scand. 1975; 581 (Suppl.)
        • Carlson L.A.
        • Ericsson M.
        Quantitative and qualitative serum lipoprotein analysis, Part 2 (Studies in male survivors of myocardial infarction).
        Atherosclerosis. 1975; 21: 417
        • Lewis B.
        • Chait A.
        • Oakley C.H.
        • Wootton I.D.P.
        Serum lipoprotein abnormalities in patients with ischaemic heart disease-Comparison with a control population.
        Brit. Med. J. 1974; III: 489
        • Beach K.W.
        • Brunzell J.D.
        • Conquest L.L.
        • Strandness D.E.
        The correlation of arteriosclerosis obliterans with lipoproteins in insulin dependent and non-insulin dependent diabetes.
        Diabetes. 1979; 28: 836
        • Nikkilä E.A.
        Triglyceride metabolism in diabetes mellitus.
        Progr. Biochem. Pharmacol. 1973; 8: 271
        • Nikkilä E.A.
        • Kekki M.
        Plasma triglyceride transport kinetics in diabetes mellitus.
        Metabolism. 1973; 12: 1
        • Ostman J.
        Studies in vitro on fatty acid metabolism of human subcutaneous adipose tissue in diabetes mellitus.
        Acta Med. Scand. 1965; 177: 639
        • Nikkilä E.A.
        • Huttunen J.K.
        • Enholm C.
        Relationship to plasma triglyceride of post heparin plasma lipoprotein lipase and hepatic lipase in diabetes mellitus.
        Diabetes. 1977; 26: 11
        • Björntorp P.
        • Hood B.
        Studies on adipose tissue from obese patients with or without diabetes mellitus, Part 1 (Release of glycerol and free fatty acids).
        Acta Med. Scand. 1966; 179: 221
        • Rossner S.
        Studies on an intravenous fat tolerance test—Methodological, experimental and clinical experiences with Intralipida®.
        Acta Med. Scand. 1975; 564 (Suppl.)
        • Lewis B.
        • Mancini M.
        • Mattock M.
        • Chait A.
        • Russel Fraser T.
        Plasma triglyceride and fatty acid metabolism in diabetes mellitus.
        Europ. J. Clin. Invest. 1972; 2: 445
        • Persson B.
        Lipoprotein lipase activity of human adipose tissue in health and in some diseases with hyperlipidemia as a common feature.
        Acta Med. Scand. 1973; 193: 457
        • Pykalisto O.J.
        • Smith P.H.
        • Brunzell J.D.
        Determinants of human adipose tissue lipoprotein lipase-Effect of diabetes and obesity on basal and diet-induced activity.
        J. Clin. Invest. 1975; 56: 1108
        • Carlson L.A.
        • Walldius G.
        Fatty acid incorporation into human adipose tissue in hypertriglyceridemia.
        Europ. J. Clin. Invest. 1976; 6: 195
        • Rubba P.
        Fractional fatty acid incorporation into human adipose tissue (FIAT) in hypertriglyceridemia.
        Atherosclerosis. 1978; 29: 39
        • Walldius G.
        • Rubba P.
        A micromethod for determination of fatty acid (FIAT) and glucose (GLIAT) incorporation and lipolysis in vitro in needle biopsies of human adipose tissue.
        Scand. J. Clin. Lab. Invest. 1976; 46: 358
        • Walldius G.
        Fatty acid incorporation into human adipose tissue (FIAT) in hypertriglyceridemia—Methodological, clinical and experimental studies.
        Acta Med. Scand. 1976; 591 (Suppl.)
        • Rubba P.
        • Pezzella G.
        • Postiglione A.
        • Mancini M.
        Fatty acid incorporation into human adipose tissue in maturity onset diabetes and a patient with an insulinoma.
        in: Crepaldi G. Lefebvre P.I. Alberti K.G.M.M. Diabetes, Obesity and Hyperlipidemias. Academic Press, London1978: 223
        • Hjelm M.
        Enzymatic determination of hexoses in blood and urine.
        Scand. J. Clin. Lab. Invest. 1966; 18: 85
        • Block W.D.
        • Jarret K.J.
        • Levine J.B.
        An improved automated determination of serum total cholesterol with a simple reagent.
        Clin. Chem. 1966; 12: 681
        • Kessler G.
        • Lederer H.
        Fluorimetric measurement of triglycerides.
        in: Skegg L.T. Automation in Analytical Chemistry. Mediad Inc, New York, NY1965: 341
        • Dole V.P.
        The fatty acid pool in adipose tissue.
        J. Biol. Chem. 1961; 236: 3121
        • Service F.J.
        • Dale A.J.D.
        • Elveback L.R.
        • Jiang N.S.
        Insulinoma—Clinical and diagnostic features of 60 consecutive cases.
        in: Proc. Mayo Clin.51. 1976: 417
        • Gries F.A.
        • Steinke J.
        Insulin and human adipose tissue—A brief review.
        Metabolism. 1967; 16: 693
        • Galton D.J.
        • Wilson J.P.D.
        • Kissebah A.
        The effect of adult diabetes on glucose utilization and esterification of palmitate by human adipose tissue.
        Europ. J. Clin. Invest. 1971; 1: 399
        • Clifton Bligh T.
        • Galton D.J.
        The esterification of exogenous fatty acids in hypertriglyceridemia with or without diabetes mellitus.
        Clin. Sci. Mol. Med. 1976; 51: 257
        • Björntorp P.
        Studies on adipose tissue from obese patients with or without diabetes mellitus, Part 2 (Basal and insulin stimulated glucose metabolism).
        Acta Med. Scand. 1966; 179: 229
        • Galton D.J.
        • Wilson J.P.D.
        A defect of glucose utilization in adipose tissue of adult diabetics in some conditions which may predispose to diabetes.
        Clin. Sci. 1970; 38: 661
      1. Walldius, G., Theve, N.O. and Rubba, P., Inverse correlation between the rate of lipolysis and fatty acid incorporation into human adipose tissue (FIAT) in vitro—Studies with stimulation and inhibition of lipolysis, Submitted for publication.

        • Luft R.
        • Wajngot A.
        • Efendic S.
        On the pathogenesis of maturity-onset diabetes.
        Diabetes Care. 1981; 4: 58