Long-term effect of hyperlipidemic serum on the synthesis of glycosaminoglycans and on the rate of growth of rabbit aortic smooth muscle cells in culture

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      Rabbit aortic smooth muscle cells (SMCs) were successfully subcultured in 10% hyperlipidemic rabbit serum (HLS) for at least 9 passages. SMCs grown in HLS grew into higher cell densities than SMCs cultured in normolipidemic rabbit serum (NLS) for at least 4–5 passages in NLS and HLS, respectively. However, cells cultured in NLS and HLS for up to 7 passages had similar growth characteristics when they were trypsinised and seeded to grow in 10% fetal calf serum (FCS).
      Incorporation of [3H]glucosamine into GAGs was taken to represent their rate of synthesis. As compared with cultures incubated in 10% NLS, incubation of rabbit aortic SMCs in the presence of 10% HLS increased the synthesis of sulphated GAGs secreted into the pericellular space by 35% during the first 24 h of contact with HLS. After preincubation for one week in the presence of HLS the synthesis of sulphated GAGs secreted into the incubation medium and into the pericellular space was stimulated by 95% and 34%, respectively. The stimulation of the synthesis of sulphated GAGs by HLS continued for up to 4 weeks at least if the contact of the cells with HLS was maintained. When the cells were subcultured in the presence of NLS and HLS and seeded to grow in FCS after the 1 st, 3rd and 7th trypsinisations, the synthesis of sulphated GAGs in cultures of cells from both sources was similar.


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        • Buck R.C.
        • Heagy F.C.
        Uptake of radioactive sulphur by various tissues of normal and cholesterol-fed rabbits.
        Canad. J. Biochem. Physiol. 1958; 36: 63
        • Forman D.T.
        • Choi S.S.
        • Taylor B.
        Sulfate content of aortic wall on experimentally induced atherosclerosis.
        Arch. Path. 1968; 85: 80
        • Ichida T.
        • Kalant N.
        Aortic glycosaminoglycans in atheroma and alloxan diabetes.
        Canad. J. Biochem. 1968; 46: 249
        • Pietila K.
        • Ylä-Herttuala S.
        • Jaakkola O.
        • Nikkari T.
        Metabolism of glycosaminoglycans and lipids in smooth muscle cells from atherosclerotic rabbit aortas in culture.
        Atherosclerosis. 1980; 37: 449
        • Järveläinen H.
        • Rönnemaa T.
        • Tammi M.
        • Vihersaari T.
        • Lehtonen A.
        • Viikari J.
        Type IIA hyperlipoproteinemic sera decrease the synthesis of hyaluronic acid by cultured human aortic smooth muscle cells.
        Atherosclerosis. 1981; 39: 61
        • Tammi M.
        • Rännemaa T.
        • Vihersaari T.
        • Saarni H.
        • Lehtonen A.
        • Viikari J.
        Effects of sera from hyperlipidemic subjects and high-density-lipoproteinemic runners on the synthesis of DNA and glycosaminoglycans by cultured human aortic smooth muscle cells.
        Med. Biol. 1979; 57: 118
        • Erlich K.
        • Murray M.
        Effect of low density lipoprotein on proteoglycan synthesis by aorta cells in culture.
        Experientia (Basel). 1978; 34: 179
        • Pillai S.
        • Donnelly P.V.
        • Mourao P.A.S.
        • DiFerrante N.
        • Eskin S.G.
        Effect of high density lipoproteins and low density lipoproteins on the glycosaminoglycans metabolism of cultured bovine smooth muscle cells.
        Biochem. Internat. 1980; 1: 55
        • Sudhakaran P.R.
        • Prinz R.
        • Filipovic L.
        • von Figura K.
        • Buddecke E.
        Homologous low density lipoprotein does not affect proteoglycan metabolism of cultured skin fibroblasts and arterial smooth muscle cells.
        Hoppe-Seyler's Z. Physiol. Chem. 1980; 361: 129
        • Pietila K.
        • Nikkari T.
        Hyperlipidemic rabbit serum reduces the recovery of acidic glycosaminoglycans from tissue culture medium.
        Anal. Biochem. 1981; 116: 317
        • Saarni H.
        • Tammi M.
        A rapid method for separation and assay of radiolabeled mucopolysaccharides from cell culture medium.
        Anal. Biochem. 1977; 81: 40
        • Chen R.M.
        • Getz G.S.
        • Fischer-Dzoga K.
        • Wissler R.W.
        The role of hyperlipidemic serum on the proliferation and necrosis of aortic medial cells in vitro.
        Exp. Mol. Path. 1977; 26: 359
        • Ledet T.
        • Fischer-Dzoga K.
        • Wissler R.W.
        Growth of aortic smooth-muscle cells cultured in media containing diabetic and hyperlipidemic serum.
        Diabetes. 1976; 25: 207
        • Pietila K.
        • Nikkari T.
        Enhanced growth of smooth muscle cells from atherosclerotic rabbit aortas in culture.
        Atherosclerosis. 1980; 34: 241
        • Nikkari T.
        • Pietila K.
        • Salo M.
        Increased cholesterol content and esterification in rabbit aortic medial cells cultured in hyperlipidemic serum.
        Med. Biol. 1976; 54: 264
        • Pietila K.
        Incorporation of [3H]glucosamine into glycosaminoglycans in different cell culture conditions by rabbit aortic smooth muscle cells.
        Biochem. Biophys. Acta. 1981; 677: 318
        • Lowry O.H.
        • Rosebrough N.J.
        • Farr A.L.
        • Randall R.J.
        Protein measurement with the Folin phenol reagent.
        J. Biol. Chem. 1951; 193: 265
        • Pollack R.
        • Vogel A.
        Isolation and characterisation of revertant cell lines, Part 2 (Growth control of a polyploid revertant line derived from SV40-transformed 3T3 mouse cells).
        J. Cell. Physiol. 1973; 82: 93
        • Holderbaum D.
        • Ehrhart L.A.
        Inhibition of collagen and non-collagen protein synthesis in cultured aortic smooth muscle cells by hyperlipoproteinemic serum.
        Artery. 1980; 7: 16
        • Pietila K.
        • Nikkari T.
        Enhanced synthesis of collagen and total protein in smooth muscle cells from atherosclerotic rabbit aortas in culture.
        Atherosclerosis. 1980; 37: 11
        • Hauss W.H.
        • Mey J.
        • Schulte H.
        Effect of risk factors and antirheumatic drugs on the proliferation of aortic wall cells.
        Atherosclerosis. 1979; 34: 119