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Apoprotein S, a family of human serum lipoprotein polypeptides

  • Claude L. Malmendier
    Correspondence
    To whom correspondence should be sent.
    Affiliations
    Research Unit on Atherosclerosis, Laboratory of Chemical Pathology, Faculty of Medicine, and Department of Clinical Chemistry, St-Pierre University Hospital, University of Brussels, Brussels, Belgium
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  • Jean P. Ameryckx
    Affiliations
    Research Unit on Atherosclerosis, Laboratory of Chemical Pathology, Faculty of Medicine, and Department of Clinical Chemistry, St-Pierre University Hospital, University of Brussels, Brussels, Belgium
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      Abstract

      Glucose infusions given to neurological and postsurgical patients in the absence of oral feeding were found to increase the amount of new polypeptides in high density lipoproteins from 3 to 40% of total proteins as compared to 0.1% in normal subjects fed a regular diet. This increase was observed in HDL2 as well as in HDL3 and even in VLDL. Eight polymorphic forms were detected by chromatography, polyacrylamide gel electrophoresis and isoelectric focusing. The partial amino acid sequence of one of these forms is given: the first 26 NH2-terminal residues are identical to the amphipathic helical segment of SAA protein, theoretically responsible for the lipid binding.
      The role of glucose as the major factor involved in the production of these apoproteins is discussed.

      Keywords

      Abbreviations:

      HDL (high density lipoproteins (1.063<d<1.210) [HDL2 (1.063<d<1.125), HDL3 (1.125<d< 1.210)]), IEF (isoelectric focusing), PAGE (polyacrylamide gel electrophoresis), SAA (amyloid protein A-related serum component), Sx (apoprotein S or DEAF-Sephacel subfraction x of Sephacryl-Fraction V), VLDL (very low density lipoproteins (d < 1.006))
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      References

        • Malmendier C.L.
        • Christophe J.
        • Ameryckx J.P.
        Separation and partial characterization of new apoproteins from human plasma high density lipoproteins.
        Clin. Chim. Acta. 1979; 99: 167
        • Malmendier C.L.
        • Paroutaud P.
        • Ameryckx J.P.
        Partial amino acid sequence of three new apolipoproteins isolated from human high density lipoproteins.
        FEBS Lett. 1980; 109: 43
        • Scanu A.
        Forms of human serum high density lipoprotein protein.
        J. Lipid Res. 1966; 7: 295
      1. Chromatofocusing with polybuffer™ and PBE™, Pharmacia Fine Chemicals AB, Box 175, S-75104 Uppsala 1, Sweden.

        • Fehrnström H.
        • Moberg U.
        SDS and conventional polyacrylamide gel electrophoresis with LKB 2117 Multiphor.
        in: LKB Application Note 306. LKB-Produkter AB, Bromma, Sweden1977
        • Winter A.
        • Ek K.
        • Andersson U.-B.
        Analytical electrofocusing in thin layers of polyacrylamide gels.
        in: Application Note 250. LKB-Produkter AB, Bromma, Sweden1977
        • Lipid Research Clinics Program
        Manual of Laboratory Operations. Vol. 1. U.S. Government Printing Office, Washington, DC1974
        • Schacterle G.R.
        • Pollack R.L.
        A simplified method for the quantitative assay of small amounts of protein in biological material.
        Anal. Biochem. 1973; 51: 654
        • Avila E.M.
        • Lopez F.
        • Camejo G.
        Properties of low density lipoproteins related to its interaction with arterial wall components—In vitro and in vivo studies.
        Artery. 1978; 4: 36
        • Lim C.T.
        • Chung J.
        • Kayden H.J.
        • Scanu A.M.
        Apoproteins of human serum high density lipoproteins—Isolation and characterization of the peptides of Sephadex Fraction V from normal subjects and patients with abetalipoproteinemia.
        Biochim. Biophys. Acta. 1976; 420: 332
        • Segrest J.P.
        • Pownall H.J.
        • Jackson R.L.
        • Glenner G.G.
        • Pollock P.A.
        Amyloid A—Amphipathic helixes and lipid binding.
        Biochemistry. 1976; 15: 3187
        • Malmendier C.L.
        • Ameryckx J.P.
        S-peptides—New apoproteins of human high density lipoproteins induced by glucose infusions.
        Clin. Chim. Acta. 1981; 111 (Short communication): 267
        • Ameryckx J.P.
        • Vanderminnen R.
        • Malmendier C.L.
        Effect of an oral diet exclusively composed of carbohydrates on high density lipoprotein composition.
        Acta Cardiol. 1981; (In press)
        • Mahley R.W.
        • Innerarity T.L.
        • Bersot T.P.
        • Lipson A.
        • Margolis S.
        Alterations in human high density lipoproteins, with or without increased plasma-cholesterol, induced of diets high in cholesterol.
        Lancet. 1978; ii: 807