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Proteolipid and bovine aorta calcification, in vitro

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      Abstract

      Proteolipid was examined for nucleation of bovine aorta calcification. Tissue homogenates were tested for in vitro calcifiability before and after lipid extraction followed by similar tests of the extract and its fractions. The tissue calcified before but not after lipid extraction. Of the lipid fractions, only proteolipid calcified. Within the constraints imposed, proteolipid is required for initiation of bovine aorta calcification, in vitro.

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      References

        • Blumenthal H.T.
        • Lansing A.I.
        • Wheeler P.A.
        Calcification of the media of the human aorta and its relation to intimal arteriosclerosis, ageing and disease.
        Amer. J. Path. 1944; 20: 665
        • Lansing A.I.
        • Alex M.
        • Rosenthal T.B.
        Calcium and elastin in human arteriosclerosis.
        J. Gerontol. 1950; 5: 112
        • Moon J.Y.
        Factors affecting arterial calcification associated with atherosclerosis.
        Atherosclerosis. 1972; 16: 119
        • Rosenblum I.Y.
        • Flora L.
        • Eisenstein R.
        The effect of disodium ethane-1-hydroxy-1,1-diphosphonate (EHDP) on a rabbit model of atherosclerosis.
        Atherosclerosis. 1975; 22: 411
        • Kramsch D.M.
        • Aspen A.J.
        • Rozler L.J.
        Atherosclerosis — Prevention by agents not affecting abnormal levels of blood lipids.
        Science. 1981; 213: 1511
        • Carlström D.
        • Engfeldt B.
        • Engström A.
        • Ringertz N.
        Studies on the chemical composition of normal and abnormal blood vessel walls.
        Lab. Invest. 1953; 2: 325
        • Weismann G.
        • Weismann S.
        X-ray diffraction studies of human aortic elastic residues.
        J. Clin. Invest. 1960; 39: 1957
        • Ennever J.
        • Vogel J.J.
        • Rider L.J.
        • Boyan-Salyers B.
        Nucleation of microbiologic calcification by proteolipid.
        in: Proc. Soc. Exp. Biol. Med.152. 1976: 147
        • Ennever J.
        • Vogel J.J.
        • Riggan L.J.
        • Paoloski S.B.
        Proteolipid and calculus matrix calcification in vitro.
        J. Dent. Res. 1977; 56: 147
        • Ennever J.
        • Boyan-Salyers B.
        • Riggan L.J.
        Proteolipid and bone matrix calcification in vitro.
        J. Dent. Res. 1977; 56: 967
        • Ennever J.
        • Vogel J.J.
        • Riggan L.J.
        Phospholipids of a bone matrix calcification nucleator.
        J. Dent. Res. 1978; 57: 731
        • Streckfuss J.L.
        • Vogel J.J.
        • Brown L.R.
        Characterization of calcifiable proteolipid from Streptococcus mutans.
        J. Dent. Res. 1981; 60: 1987
        • Ennever J.
        • Vogel J.J.
        • Riggan L.J.
        Calcification by proteolipid from atherosclerotic aorta.
        Atherosclerosis. 1980; 35: 209
        • Simpson R.J.
        • Neuberger M.R.
        • Liu T.Y.
        Complete amino acid analysis of proteins from a single hydrolysate.
        J. Biol. Chem. 1976; 251: 1936
        • Horning M.G.
        • Murakima C.
        • Horning E.C.
        Analysis of phospholipids, ceramides and cerebrosides by gas chromatography-mass spectrometry.
        Amer. J. Clin. Nutrition. 1971; 24: 1086
        • Folch J.
        • Lees M.
        Proteolipids, a new type of tissue lipoproteins — Their isolation from brain.
        J. Biol. Chem. 1951; 191: 807
        • Folch-Pi J.
        • Stoffyn P.J.
        Proteolipids from membrane systems.
        Ann. N.Y. Acad. Sci. 1972; 195: 86
        • Lees M.B.
        • Sakura J.D.
        • Sapirstein V.S.
        • Curatolo W.
        Structure and function of proteolipids in myelin and non-myelin membranes.
        Biochim. Biophys. Acta. 1979; 559: 209
        • Schlesinger M.J.
        Proteolipids.
        Ann. Rev. Biochem. 1981; 50: 193