Serum lecithin: Cholesterol acyltransferase activities of cynomolgus monkeys fed different carbohydrate diets

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      Although serum lecithin: cholesterol acyltransferase (LCAT) activity is known to be modulated by nutritional factors, little is known about the effects of dietary carbohydrate on this enzyme. Therefore, LCAT activities were assessed in cynomolgus monkeys fed diets for 6 weeks on 4 diets containing 77% of calories as sucrose or starch and cholesterol at 0 and 1 mg/kcal. Three different assay conditions were used in order to measure the overall LCAT activity and to differentiate enzyme activity from the effect of serum substrate and end-product lipoprotein alterations by diet on this enzyme. Molar rate of serum total LCAT activity was higher in sucrose than starch diets (P < 0.01). Use of sera from sucrose-fed animals, either as substrate or enzyme source, increased the fractional rate of cholesterol esterificatio (P < 0.01). Exogenous cholesterol lowered serum total LCAT activity only in sucrose diet (P < 0.01). Use of sera from sucrose + cholesterol-fed animals as substrate significantly lowered the fractional rate of cholesterol esterification (P < 0.01); whereas no such alterations were noted when this serum was used as enzyme source. The differential effect of starch and sucrose diets on LCAT activity suggests that the nature of dietary carbohydrates may affect LCAT activity in association with triglyceride metabolism by altering the amount of enzyme in terms of its activity and/or the nature of substrate and cholesterol ester acceptor lipoproteins.


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        • Glomset J.A.
        Lecithin: cholesterol acyltransferase — An exercise in comparative biology.
        in: 65th edition. Progress in Biochemical Pharmacology. Vol. 15. Karger, Basel, New York1979: 41
        • Chajek T.
        • Fielding C.J.
        Isolation and characterization of human serum cholesterol ester transfer proteins.
        in: 65th edition. Proc. Nat. Acad. Sci. (USA). 75. 1978: 3445
        • Fielding C.J.
        • Shore V.G.
        • Fielding P.E.
        A protein cofactor of lecithin-colesterol acyltransferase.
        Biochem. Biophys. Res. Commun. 1972; 46: 1493
        • Fielding C.J.
        • Fielding P.E.
        Regulation of human plasma lecithin: cholesterol acyltransferase activity by lipoprotein acceptor cholesterol ester content.
        J. Biol. Chem. 1981; 256: 2102
        • Mahley R.W.
        Dietary fat, cholesterol and accelerated atherosclerosis.
        in: 65th edition. Atherosclerosis Reviews. Vol. 5. Raven Press, New York, NY1979: 1
        • Schonfeld G.
        • Weidman S.W.
        • Witztum J.L.
        • Bowen R.M.
        Alterations in the levels and interrelations of plasma apoproteins induced by diet.
        Metabolism. 1976; 25: 261
        • Srinivasan S.R.
        • Clevidence B.A.
        • Pargaonkar P.S.
        • Radhakrishnamurthy B.
        • Bersenson G.S.
        Varied effects of dietary sucrose and cholesterol on serum lipids, lipoproteins and apoproteins in rhesus monkeys.
        Atherosclerosis. 1979; 33: 301
        • Srinivasan S.R.
        • Radhakrishnamurthy B.
        • Webber L.S.
        • Dalferes Jr., E.R.
        • Kokatnur M.G.
        • Berenson G.S.
        Synergistic effects of dietary carbohydrate and cholesterol on serum lipids and lipoproteins in squirrel and spider monkeys.
        Amer. J. Clin. Nutr. 1978; 31: 603
        • Lichtenstein A.H.
        • Nicolosi R.J.
        • Hayes K.C.
        Dietary fat and cholesterol effects on plasma lecithin: cholesterol acyltransferase activity in cebus and squirrel monkeys.
        Atherosclerosis. 1980; 37: 603
      1. 65th edition. Manual of Laboratory Operations, Lipid Research Clinics Program (DHEW Publication, No. NIH75-628). National Heart, Lung and Blood Institute, Bethesda, MD1974
        • Folch J.
        • Lees M.
        • Sloane-Stanley G.H.
        A simple method for the isolation and purification of total lipids from animal tissues.
        J. Biol. Chem. 1957; 226: 497
        • Rudel L.L.
        • Morris M.D.
        Determination of cholesterol using o-phthalaldehyde.
        J. Lipid Res. 1964; 14: 364
        • Bhandaru R.R.
        • Srinivasan S.R.
        • Pargaonkar P.S.
        • Berenson G.S.
        A simplified colorimetric micromethod for determination of serum cholesterol.
        Lipids. 1977; 12: 1078
        • Stokke K.T.
        • Norum K.R.
        Determination of lecithin: cholesterol acyltransferase in human blood plasma.
        Scand. J. Clin. Lab. Invest. 1971; 27: 21
        • Glomset J.A.
        • Wright J.L.
        Further studies on the mechanism of plasma cholesterol identification reaction.
        Biochim. Biophys. Acta. 1970; 70: 389
        • Soloff L.
        • Rutenberg H.
        • Jacko H.
        Serum cholesterol esterification in patients with coronary heart disease.
        Amer. Heart J. 1973; 85: 153
        • Snedecor G.W.
        • Cochran W.A.
        Statistical Methods.
        65th edition. Iowa State University Press, Ames, IA1967
        • Bhattacharya A.K.
        Plasma lecithin: cholesterol acyltransferase activity in high and low responders.
        Experientia (Basel). 1981; 37: 1250
        • MacDonald I.
        Effects of carbohydrates on lipid metabolism.
        in: 6th edition. Progress in Biochemical Pharmacology. Vol. 8. Karger, Basel, New York1973: 1-290
        • Nestel P.J.
        Triglyceride turnover in man.
        in: 6th edition. Progress in Biochemical Pharmacology. Vol. 8. Karger, Basel, New York1973: 125
        • Wallentin L.
        • Vikrot O.
        Influence of fat ingestion on lecithin: cholesterol acyltransferase rate in plasma of normal persons.
        Scand. J. Clin. Lab. Invest. 1976; 36: 473
        • Kudchodkar B.J.
        • Sodhi H.S.
        Turnover of plasma cholesterol esters and its relationship to other parameters of lipid metabolism in man.
        Europ. J. Clin. Invest. 1976; 6: 285
        • Wallentin L.
        Influence of intravenous and oral administration of phospholipids on lecithin: cholesterol acyltransferase rate in plasma.
        Artery. 1977; 3: 40
        • Rose H.G.
        • Juliano J.
        Regulation of plasma lecithin: cholesterol acyltransferase, Part 2 (Activation during alimentary lipemia).
        J. Lab. Clin. Med. 1977; 89: 524
        • Rose H.G.
        • Juliano J.
        Regulation of plasma lecithin: cholesterol acyltransferase, Part 1 (Increased activity in primary hypercholesterolemia).
        J. Lab. Clin. Med. 1976; 88: 29
        • Nordby G.
        • Berg T.
        • Nillson M.
        • Norum K.R.
        Secretion of lecithin:cholesterol acyltransferase from isolated rat hepatocytes.
        Biochim. Biophys. Acta. 1976; 450: 69
        • Weber G.
        • Singhal R.L.
        • Stanum N.B.
        • Fisher E.A.
        • Mentendiek M.A.
        Regulation of enzymes involved in gluconeogenesis.
        Adv Enzyme Regulation. 1964; 2: 1
        • Markle W.
        • Lang C.M.
        • Rosenberg A.
        • Barthel C.H.
        Cholesterol ester content of the serum — Alterations due to simple and complex carbohydrate diets in nonhuman primates (Cebus albifrons, Macaca arctoides and Macaca mulatta ).
        Biochem. Med. 1971; 5: 205
        • Groener J.E.M.
        • Klein W.
        • Van Golde L.M.G.
        The effect of fasting and refeeding on the composition and synthesis of triacylglycerols, phosphatidylcholines and phosphatidylethanolamines in rat liver.
        Arch. Biochem. Biophys. 1979; 198: 287