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Abstract
Atherosclerotic plaque formation is accompanied by hyperproliferative events which
have many features of an inflammatory response. A high-performance liquid chromatography
procedure was developed to analyze the inflammatory prostaglandins, leukotrienes and
hydroxyeicosatetraenoic acids (HETEs) produced by aortic segments. Normal rabbit aortas
incubated with tritiated arachidonic acid synthesized 12-HETE as the principal lipoxygenase
metabolite, and prostacyclin as the major cyclooxygenase product. In contrast, atherosclerotic
aortas from both cholesterol-fed and Watanabe Heritable Hyperlipidemic rabbits showed
major increases in synthesis of lipoxygenase-derived 15-HETE, which became the predominant
eicosanoid in the aortas of both types of rabbit. No leukotrienes or other 5-lipoxygenase
products were detected to the detection limit of 0.5 pmol/cm aorta. 15-HETE, which
is chemotactic for smooth muscle cells, mitogenic for endothelial cells, and an inhibitor
of prostacyclin synthesis may thus play a role in atherogenesis.
Keywords
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Article info
Publication history
Accepted:
August 8,
1988
Received in revised form:
August 3,
1988
Received:
February 15,
1988
Identification
Copyright
© 1989 Published by Elsevier Inc.