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Reduced stimulatory activity on prostacyclin production by cultured endothelial cells in serum from aged and diabetic patients

  • F. Umeda
    Correspondence
    Correspondence to: Dr. Fumio Umeda, Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812, Japan.
    Affiliations
    Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka 812, Japan
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  • T. Inoguchi
    Affiliations
    Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka 812, Japan
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  • H. Nawata
    Affiliations
    Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka 812, Japan
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      Abstract

      Reduced prostacyclin (PGI2) generation by the vascular wall shows a close relationship with the development of atherosclerosis. The present study found plasma-derived serum (PDS) to contain an activity which stimulated PGI2 production by cultured bovine aortic endothelial cells. Diabetic and aged patients with atherosclerotic disease were examined for abnormalities in that stimulatory activity in PDS. PDS obtained from both diabetics (NIDDM) and aged patients showed a significant reduction in the stimulation of PGIZ production by cultured bovine aortic endothelial cells compared with age-matched controls and young healthy volunteers, respectively. It was suggested that the reduced PGI2 stimulatory activity in PDS may be one of the pathogenic mechanisms of vascular lesions such as atherosclerosis in diabetics and aged humans.

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      References

        • Sinzinger H.
        • Feigl W.
        • Silberbauer K.
        Prostacyclin generation in atherosclerotic arteries.
        Lancet. 1979; ii: 469
        • Masotti G.
        • Poggesi L.
        • Galanti G.
        • Trottf F.
        • Neri Senneri G.G.
        Prostacyclin production in man.
        in: Lewis P.J. O'Grady J. Clinical Pharmacology of Prostacyclin. Raven Press, New York1981: 9
        • MacIntyre D.E.
        • Pearson J.D.
        • Gordon J.L.
        Localization and stimulation of prostacyclin production in vascular cells.
        Nature. 1978; 271: 549
        • Inoguchi T.
        • Umeda F.
        • Watanabe J.
        • Ibayashi H.
        Reduced serum-stimulatory activity on prostacyclin production by cultured aortic endothelial cells in diabetes mellitus.
        Haemostasis. 1986; 16: 447
        • Inoguchi T.
        • Umeda F.
        • Watanabe J.
        • Ibayashi H.
        Stimulatory activity on prostacyclin production decreases in sera from streptozotocin-induced diabetic rats.
        Diabetes Res. Clin. Pract. 1987; 3: 243
        • Pledger W.J.
        • Stiles C.D.
        • Antoniades H.N.
        • Scher C.D.
        Induction of DNA synthesis in Balb/c-3T3 cells by serum components: reevaluation of the commitment process.
        in: 7th edn. Proc. Natl. Acad. Sci. USA. 74. 1977: 4481
        • Jaffe B.M.
        • Behrman H.R.
        • Parker C.W.
        Radioimmunoassay measurement of prostaglandins E, A, and F in human plasma.
        J. Clin. Invest. 1973; 52: 398
        • Uyama O.
        • Nagatsuka K.
        • Nakamura M.
        • Matsumoto M.
        • Fujisawa A.
        • Yoneda S.
        • Kimura K.
        • Abe H.
        Plasma concentrations of 6-keto-prostaglandin Fl. in patients with hypertension, cerebrovascular disease or Takayasu's arteritis.
        Thromb. Res. 1982; 25: 71
        • Inoue T.
        Measured effects and clinical correlations of antiplatelet agents on plasma levels of thromboxane A 2 and 6-keto-prostaglandin F1a and fatty acid composition of platelets in ischemic heart disease and cerebral infarction.
        J. Jap. Geriatr. Med. 1983; 20: 294
        • Dollery C.T.
        • Friedman L.A.
        • Hensby C.N.
        • Kohner E.
        • Lewis P.J.
        • Porta M.
        • Webster J.
        Circulating prostacyclin may be reduced in diabetes.
        Lancet. 1979; ii: 1365
        • Silberbauer K.
        • Schernthaner G.
        • Sinzinger H.
        • PizaKatzer H.
        • Winter M.
        Decreased vascular prostacyclin in juvenile-onset diabetes.
        N. Engl. J. Med. 1979; 300: 366
        • Watanabe J.
        • Umeda F.
        • Sugimoto H.
        • Wasada T.
        • Ibayashi H.
        Decreased prostacyclin production and platelet abnormalities in diabetes mellitus.
        J. Jap. Diabet. Soc. 1985; 28: 1229
        • Dembinska-Kiec A.
        • Gryglewski T.
        • Zmuda A.
        • Gryglewski R.J.
        The generation of prostacyclin by arteries and by the coronary vascular bed is reduced in experimental atherosclerosis in rabbits.
        Prostaglandins. 1977; 14: 1025
        • Chang W.C.
        • Tai H.H.
        Changes in arachidonate metabolism in aortas and platelets in aging rats.
        Prostaglandins Leukotrienes Med. 1983; 12: 149
        • Aanderud S.
        • Krane K.
        • Nordy A.
        Influence of glucose, insulin and sera from diabetic patients on the prostacyclin synthesis in vitro in cultured human endothelial cells.
        Diabetologia. 1985; 28: 641
        • Coughlin S.R.
        • Moskowitz M.A.
        • Zetter B.R.
        • Antoniades H.N.
        • Levine L.
        Platelet-dependent stimulation of prostacyclin synthesis by platelet-derived growth factor.
        Nature. 1980; 288: 600
        • Ritter J.M.
        • Ongari M.A.
        • Orchard M.A.
        • Lewis P.J.
        Prostacyclin synthesis is stimulated by a serum factor formed during coagulation.
        Thrombos. Haemostas. 1983; 49: 58