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Adhesion of leukocytes to the aortic endothelium of conventional, specific pathogen free (SPF) and hypercholesterolemic SPF rats

  • María Gabaldón
    Correspondence
    Correspondence to: Dr. Maria Gabaldón, Unidad de histoquímica, Centro de Investigation, Hospital “La Fe”, Avenida Campanar 21, E-46009 Valencia, Spain.
    Affiliations
    Unidad de Histoquimia, Centro de Investigacíon, Hospital “La Fe”, 46009 Valencia, Spain
    Search for articles by this author
  • Carmen Capdevila
    Affiliations
    Unidad de Histoquimia, Centro de Investigacíon, Hospital “La Fe”, 46009 Valencia, Spain
    Search for articles by this author
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      Abstract

      Adhesion of leukocytes to the aortic endothelium was studied in specific pathogen-free (SPF) and conventional rats and in SPF rats with diet-induced hypercholesterolemia. Nonspecific esterase activity with α-naphthyl acetate as substrate was used to characterize the adhered cells. Phagocytic activity was determined by injecting i.v. 0.1–0.4 ml/100 g doses of Monastral blue B® (MbB). Adhesion in SPF rats was 8 ± 4 esterase (+) cells/mm2. Adhesion in conventional rats was of the same order except in 2 cases with antibodies to Mycoplasma pulmonis and Kilham rat virus, where adhesion was 44 and 68 esterase ( + ) cells/mm2, respectively. For all MbB doses studied, phagocytic activity arose in a percentage of the adherent cells, ranging from 5 to 85%. Rats fed the hyperlipidic diet for 15 days developed severe hypercholesterolemia and adhesion was drastically increased to 200–700 esterase (+) cells/mm2. Results indicate that: (1) spontaneous pathology in rats may produce an increased adhesion of leukocytes to the endothelium, and (2) phagocytic activity is only expressed in a fraction of the esterase (+) cells adhered to the endothelium.

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