Advertisement
Research paper| Volume 99, ISSUE 2, P151-163, March 01, 1993

Relations of sodium-lithium countertransport kinetics to plasma and red cell membrane phospholipids in hyperlipidemia

DOI:https://doi.org/10.1016/0021-9150(93)90018-P
Relations of sodium-lithium countertransport kinetics to plasma and red cell membrane phospholipids in hyperlipidemia
Previous ArticleThe atherogenic potential of carbon monoxide
Next ArticleAtherosclerosis in a rhesus monkey with genetic hypercholesterolemia and elevated plasma Lp(a)
      This paper is only available as a PDF. To read, Please Download here.

      Abstract

      As compared to 7 normolipidemic donors, the maximal velocity of sodium-lithium countertransport was accelerated by nearly 70% in 10 patients with elevated levels of triglyceride-rich lipoproteins and tended to be stimulated also in 5 patients with hypercholesterolemia. No significant differences were observed between normolipidemia and both hyperlipidemic groups for the apparent affinities of the transport system for intracellular sodium and extracellular lithium. Strong positive relations of the maximal activity of sodium-lithium countertransport to the percentages of red cell membrane phosphatidylcholine (r = 0.85, 2P < 0.001), the phosphatidylcholine/sphingomyelin (r = 0.82, 2P < 0.001) and the phosphatidylcholine/phosphatidylethanolamine ratio (r = 0.81, 2P < 0.001) were seen in all donors. A negative correlation was found to membrane sphingomyelin (r = −0.72, 2P < 0.001). Also plasma phosphatidylcholine and sphingomyelin exhibited positive and negative associations, respectively, to the maximal activity of sodium-lithium countertransport (r = 0.66, 2P < 0.01 and r = −0.78, 2P < 0.001). Among several plasma lipoprotein parameters investigated, total triglycerides or VLDL cholesterol levels showed independent relations to both the plasma and the membrane phosphatidylcholine/sphingomyelin ratio as well as to the maximal velocity of sodium-lithium countertransport. The results indicate that an increase in red cell membrane phosphatidylcholine and a concomitant fall in sphingomyelin are closely associated with the acceleration of sodium-lithium countertransport in hyperlipidemia.

      Keywords

      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Atherosclerosis
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Register: Create an account
      Institutional Access: Sign in to ScienceDirect

      References

        • Canessa M.
        • Adragna N.
        • Solomon H.S.
        • Connolly T.M.
        • Tosteson D.C.
        Increased sodium-lithium countertransport in red cells of patients with essential hypertension.
        N. Engl. J. Med. 1980; 302: 772
        • Williams R.R.
        • Hunt S.C.
        • Kuida H.
        • Ash K.O.
        Sodium-lithium countertransport in erythrocytes of hypertension prone families in Utah.
        Am. J. Epidemiol. 1983; 118: 338
        • Corrocher R.
        • Steinmayr M.
        • Ruzzenente O.
        • Brugnara C.
        • Bertinato L.
        • Mazzi M.
        • Furri C.
        • Bonfanti F.
        • de Sandre G.
        Elevation of red cell sodium-lithium countertransport in hyperlipidernias.
        Life Sci. 1985; 36: 649
        • Carr S.J.
        • Thomas T.H.
        • Laker M.F.
        • Wilkinson R.
        Elevated sodium-lithium countertransport: a familial marker of hyperlipidaemia and hypertension?.
        J. Hypertension. 1990; 8: 139
        • Mangili R.
        • Bending J.J.
        • Scott G.
        • Li L.K.
        • Gupta A.
        • Viberti G.C.
        Increased sodium-lithium countertransport activity in red cells of patients with insulindependent diabetes and nephropathy.
        N. Engl. J. Med. 1988; 318: 146
        • Carr S.
        • Mbanya J.C.
        • Thomas T.
        Increase in glomerular filtration rate in patients with insulindependent diabetes and elevated sodium-lithium countertransport.
        N. Engl. J. Med. 1990; 322: 500
        • Duhm J.
        • Becker B.F.
        Studies on the lithium transport across the red cell membrane. IV. Interindividual variations in the Nay+-dependent Li+ countertransport system of human erythrocytes.
        Pflügers Arch. 1977; 370: 211
        • Sarkadi B.
        • Alifimoff J.K.
        • Gunn R.B.
        • Tosteson D.C.
        Kinetics and stoichiometry of Na-dependent Li transport in human red blood cells.
        J. Gen. Physiol. 1978; 72: 249
        • Boerwinkle E.
        • Turner S.T.
        • Weinshilboum R.
        • Richelson E.
        • Sing C.F.
        Genetic analysis of the distribution of sodium-lithium countertransport in a sample representative of the general population.
        Gen. Epidemiol. 1986; 3: 365
        • Hasstedt S.J.
        • Wu L.L.
        • Ash O.
        • Kuida H.
        • Williams R.R.
        Hypertension and sodium-lithium countertransport in Utah pedigrees: Evidence for major locus inheritance.
        Am. J. Hum. Genet. 1988; 43: 14
        • Brent G.A.
        • Canessa M.
        • Dluhy R.G.
        Reversible alteration of red cell lithium-sodium countertransport in patients with thyroid disease.
        J. Clin. Endocrin. Metab. 1989; 68: 322
        • Adragna N.
        • Chang S.
        • Morey M.
        • Williams R.
        Effects of exercise on cation transport in human red cells.
        Hypertension. 1985; 7: 132
        • Hespel P.
        • Lijnen P.
        • Fagard R.
        • M'Buyamba-Kabangu Van Hoof R.
        • Lissens W.
        • Rosseneu M.
        • Amery A.
        Changes in erythrocyte sodium and plasma lipids associated with physical training.
        J. Hypertension. 1988; 6: 159
        • Behr J.
        • Witzgall H.
        • Lorenz R.
        • Weber P.C.
        • Duhm J.
        Red cell Na+-K+ transport in various forms of human hypertension: role of cardiovascular risk factors and plasma potassium.
        Klin. Wochenschr. 1985; 63: 63
        • Hunt S.C.
        • Williams R.R.
        • Smith J.B.
        • Ash K.O.
        Associations of three erythrocyte cation transport systems with plasma lipids in Utah subjects.
        Hypertension. 1986; 8: 30
        • Jones S.L.
        • Trevisan R.
        • Tariq T.
        • Semplicini A.
        • Mattock M.
        • Walker J.D.
        • Nosadini R.
        • Viberti G.
        Sodium-lithium countertransport in microalbuminuric insulin-dependent diabetic patients.
        Hypertension. 1990; 15: 570
        • Hunt S.C.
        • Williams R.R.
        • Ash K.O.
        Changes in sodium-lithium countertransport correlate with changes in triglyceride levels and body mass index over Math Eq years of follow-up in Utah.
        Cardiovasc. Drugs Ther. 1990; 4: 357
        • Carr S.J.
        • Thomas T.H.
        • Laker M.F.
        • Wilkinson R.
        Lipid lowering therapy leads to a reduction in sodiumlithium countertransport activity.
        Atherosclerosis. 1991; 87: 103
        • Levy R.
        • Livne A.
        The erythrocyte membrane in essential hypertension. Characterization of the temperature dependence of lithium efflux.
        Biochim. Biophys. Acta. 1984; 769: 41
        • Bing R.F.
        • Heagerty A.M.
        • Thurston H.
        • Swales J.D.
        Ion transport in hypertension: are changes in the cell membrane responsible?.
        Clin. Sci. 1986; 71: 225
        • Duhm J.
        Possible role of plasma lipids in alterations of cell membrane sodium transport in essential hypertension: pathogenetic implications.
        in: Atherosclerosis Reviews. Blood Cells and Arteries in Hypertension and Atherosclerosis. Vol. 19. Raven Press, New York1989: 247
        • Engelmann B.
        • Op den Kamp J.A.F.
        • Roelofsen B.
        Replacement of molecular species of phosphatidylcholine: influence on erythrocyte Na transport.
        Am J. Physiol. 1990; 258 (Cell Physiol 27): C682
        • Engelmann B.
        • Duhm J.
        Effect of cholesterol and dipalmitoyl phosphatidylcholine enrichment on the kinetics of Na-Li exchange of human erythrocytes.
        J. Membr. Biol. 1991; 122: 231
        • Rose H.G.
        • Oklander M.J.
        Improved procedure for the extraction of lipids from human erythrocytes.
        J. Lip. Res. 1965; 65: 428
        • Broekhuyse R.M.
        Quantitative two dimensional thinlayer chromatography of blood phospholipids.
        Clin. Chim. Acta. 1969; 23: 457
        • Gerlach E.
        • Deuticke B.
        Eine einfache Methode zur Mikrobestimmung von Phosphat in der Papierchromatographie.
        Biochem. Z. 1963; 337: 477
        • Bligh E.G.
        • Dyer W.J.
        A rapid method for total lipid extraction or purification.
        Can. J. Biochem. Physiol. 1959; 37: 911
        • Duhm J.
        • Becker B.F.
        Studies on the lithium transport across the red cell membrane. V., On the nature of the Na+-dependent Li+ countertransport system of mammalian erythrocytes.
        J. Membrane Biol. 1979; 51: 263
        • Duhm I.
        Pathways of lithium transport across the human erythrocyte membrane.
        in: Lithium Kinetics, Phar macotherapy Monographs. Vol. 1. Marius Press, Carnfort, UK,1992: 27
        • Duhm J.
        • Göbel B.O.
        Sodium-lithium exchange and sodium-potassium cotransport in human erythrocytes. Part 1: Evaluation of a simple uptake test to assess the activity of the two transport systems.
        Hypertension. 1982; 4: 468
        • Halperin J.A.
        • Brugnara C.
        • Kopin A.S.
        • Ingwall J.S.
        • Tosteson D.C.
        Properties of the Na'-K' pump in human red cells with increased number of pump sites.
        J. Clin. Invest. 1987; 80: 128
        • Hannaert P.A.
        • Garay R.P.
        A kinetic analysis of Na-Li countertransport in human red blood cells.
        J. Gen. Physiol. 1986; 87: 353
        • Bruckdorfer K.R.
        • Graham J.M.
        The exchange of cholesterol and phospholipids between cell membranes and lipoproteins.
        in: Biological Membranes. Vol. 3. Academic Press, London1976: 103
        • Worley R.J.
        • Hentschel W.M.
        • Cormier C.
        • Nutting S.
        • Pead G.
        • Zelenkov K.
        • Smith J.B.
        • Ash K.O.
        • Williams R.R.
        Increased sodium-lithium counter transport in erythrocytes of pregnant women.
        N. Engl. J. Med. 1982; 307: 412
        • Hitzemann R.
        • Hirschowitz J.
        • Garver D.
        Membrane abnormalities in the psychoses and affective disorders.
        J. Psychiat. Res. 1984; 18: 319
        • Bahrenholz Y.
        Sphingomyelin-lecithin balance in membranes: composition, structure and function relationships.
        in: Physiology of Membrane Fluidity. Vol. 1. CRC Press, Boca Raton, FL1984: 131
        • Mato J.M.
        Regulation of phospholipid methylation and its effect on membrane proteins.
        in: Progress in Protein-Lipid Interactions. 2. Elsevier Science Publishers BV, Amsterdam1986: 267
        • Engelmann B.
        • Duhm J.
        • Streich S.
        • Schönthier U.M.
        Interindividual variability in Na+ Li+ exchange activity of human erythrocytes: relation to plasma and membrane phospholipids.
        Pflügers Arch. 1992; 420 (abstract): R74
        • Yao Z.
        • Vance D.E.
        The active synthesis of phosphatidylcholine is required for very low density lipoprotein secretion from rat hepatocytes.
        J. Biol. Chem. 1988; 263: 2998
        • Yao Z.
        • Vance D.E.
        Head group specificity in the requirement of phosphatidylcholine biosynthesis for very low density lipoprotein secretion from cultured hepatocytes.
        J. Biol. Chem. 1989; 264: 11373

      Article Info

      Publication History

      Accepted: November 3, 1992
      Received in revised form: October 19, 1992
      Received: July 27, 1992

      Identification

      DOI: https://doi.org/10.1016/0021-9150(93)90018-P

      Copyright

      © 1993 Published by Elsevier Inc.

      ScienceDirect

      Access this article on ScienceDirect
      We use cookies to help provide and enhance our service and tailor content. To update your cookie settings, please visit the for this site.
      Copyright © 2022 Elsevier Inc. except certain content provided by third parties. The content on this site is intended for healthcare professionals.


      RELX