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Research paper| Volume 99, ISSUE 1, P79-86, February 1993

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Effect of testosterone on atherogenesis in cholesterol-fed rabbits with similar plasma cholesterol levels

DOI:https://doi.org/10.1016/0021-9150(93)90053-W
Effect of testosterone on atherogenesis in cholesterol-fed rabbits with similar plasma cholesterol levels
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      Abstract

      The aim was to examine the effect of testosterone on atherogenesis in cholesterol-fed, castrated male rabbits not mediated via different plasma cholesterol levels. The rabbits in the testosterone group (n = 19) and in the placebo group (n = 17) were injected intramuscularly twice weekly for 17 weeks with 25 mg testosterone enantate and placebo, respectively, reaching plasma testosterone levels of 50–150 nmol/l in the testosterone group. No effect of testosterone on liver function or body weight was detected, but at week 15 mean blood pressure was 75 ± 2 mmHg (mean ± S.E.) in the testosterone group compared with 69 ± 2 mmHg in the placebo group (P < 0.05). To reduce variation in plasma cholesterol between the two groups, the amount of cholesterol fed to each rabbit was adjusted on the basis of weekly determinations of plasma cholesterol; the mean plasma cholesterol levels during the 17 weeks were 20.9 ± 1.0 and 20.4 ± 0.9 mmol/l for the placebo and testosterone groups. In the intima-inner medias of the aortic arch, the thoracic and the abdominal aorta there were no consistent significant differences in aortic cholesterol content, expressed either as nmol/cm2 or nmol/mg protein, between the two groups. However, the aortic cholesterol content tended to be lower in the testosterone group than in the placebo group. These findings suggest that in cholesterol-fed, castrated male rabbits, testosterone does not promote atherogenesis by an effect directly on the arterial wall.

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      References

        • Study group, European Atherosclerosis Society
        The recognition and management of hyperlipedemia in adults: a policy statement of the European Atherosclerosis Society.
        Eur. Heart J. 1988; 9: 571
        • National Cholesterol Education Program
        Highlights of the report of the expert panel on detection, evaluation and treatment of high blood cholesterol in adults.
        in: 2nd Edn. Report No. NIH-88-2926. National Institutes of Health, 1987
        • Kushwaha R.S.
        • Hazzard W.R.
        Exogenous estrogens attenuate dietary hypercholesterolemia and atherosclerosis in the rabbit.
        Metabolism. 1981; 30: 359
        • Fischer G.M.
        • Cherian K.
        • Swain M.L.
        Increased synthesis of aortic collagen and elastin in experimental atherosclerosis. Inhibition by contraceptive steroids.
        Atherosclerosis. 1981; 39: 463
        • Fisher G.M.
        • Swain M.L.
        Effects of estradiol and progesterone on the increased synthesis of collagen in atherosclerotic rabbit aortas.
        Atherosclerosis. 1985; 54: 177
        • Hough J.L.
        • Zilversmit D.B.
        Effect of 17 beta estradiol on aortic cholesterol content and metabolism in cholesterol-fed rabbits.
        Arteriosclerosis. 1986; 6: 57
        • Henriksson P.
        • Stamberger M.
        • Eriksson M.
        • et al.
        Oestrogen-induced changes in lipoprotein metabolism: role in prevention of atherosclerosis in the cholesterol-fed rabbit.
        Eur. J. Clin. Invest. 1989; 19: 395
        • Haarbo J.
        • Leth-Espensen P.
        • Stender S.
        • Christiansen C.
        Estrogen monotherapy and combined estrogen-progestogen replacement therapy attenuate aortic accumulation of cholesterol in ovarieectomized cholesterol-fed rabbits.
        J. Clin. Invest. 1991; 87: 1274
        • Fischer G.M.
        • Bashey R.I.
        • Rosenbaum H.
        • Lyttle C.R.
        A possible mechanism in arterial wall for mediation of sex difference in atherosclerosis.
        Exp. Mol. Pathol. 1985; 43: 288
        • Wu Z.Y.
        • Wu X.K.
        • Zhang Y.W.
        Relationship of menopausal status and sex hormones to serum lipids and blood pressure.
        Int. J. Epidemiol. 1990; 19: 297
        • Chen Y.F.
        • Meng Q.C.
        Sexual dimorphism of blood pressure in spontaneously hypertensive rats is androgen dependent.
        Life Sci. 1991; 48: 85
        • Folch J.
        • Lees M.
        • Stanley G.H.S.
        A simple method for the isolation and purification of total lipids from animal tissues.
        J. Biol. Chem. 1957; 226: 497
        • Jaroβ W.
        • Honigmann G.
        • Kunze D.
        • Hildmann W.
        • Trübsbach A.
        Anleitungen fur Klinische Laboratoriums-methoden.
        VEB Verlag Volk and Gesundheit, Berlin1984
        • Lowry O.H.
        • Rosebrough N.J.
        • Farr A.L.
        • Randall R.J.
        Protein measurement with the folin phenol reagent.
        J. Biol. Chem. 1951; 193: 265
        • Constantinides P.
        Experimental Atherosclerosis.
        Elsevier, Amsterdam1965
        • Hácik T.
        • Hromadová M.
        Effect of short-term androgen administration to rabbit females on their lipoprotein metabolism.
        Endocrinol. Exp. 1988; 22: 181
        • Gordon G.B.
        • Bush D.E.
        • Weisman H.F.
        Reduction of atherosclerosis by administration of dehydroepiandrosterone. A study in the hypercholesterolemic New Zealand White rabbit with aortic intimal injury.
        J. Clin. Invest. 1988; 82: 712
        • Arad Y.
        • Badimon J.J.
        • Badimon L.
        • Hembree W.C.
        • Ginsberg H.N.
        Dehydroepiandrosterone feeding prevents aortic fatty streak formation and cholesterol ac cumulation in cholesterol-fed rabbit.
        Arteriosclerosis. 1989; 9: 159
        • Fogelberg M.
        • Björkhem I.
        • Diczfalusy U.
        • Henriksson P.
        Stanozolol and experimental atherosclerosis: atherosclerotic development and blood lipids during anabolic steroid therapy of New Zealand White rabbits.
        Scand. J. Clin. Lab. Invest. 1990; 50: 693

      Article info

      Publication history

      Accepted: October 29, 1992
      Received in revised form: October 28, 1992
      Received: July 23, 1992

      Identification

      DOI: https://doi.org/10.1016/0021-9150(93)90053-W

      Copyright

      © 1993 Published by Elsevier Inc.

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