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Abstract
The present study was designed to examine apolipoprotein and LDL receptor gene expression
in genetically hypercholesterolemic RICO rats. In the plasma of RICO rats as compared
to SW (control) rats, the hypercholesterolemia (+ 41%) was associated with a significant
increase in plasma apo B (+ 23%) and apo E (+ 68%) concentrations. Study of apolipoprotein
synthesis in the liver has shown that this increase in plasma apo B and apo E concentrations
was not associated with modification in their synthesis and mRNA levels. Study of
apo E mRNA level in various tissues has shown only the modification in adrenals in
RICO as compared to SW rats (2.7-fold increase). Study of LDL binding, LDL receptor
mass and LDL receptor mRNA level in the liver of RICO and SW rats has shown no significant
differences between these two strains. EDTA-resistant binding of rat LDL was lower
in RICO than in SW rats suggesting that binding sites others than the LDL receptor
are present in lesser amount in this hypercholesterolemic strain.
Keywords
Abbreviations:
apo (apolipoprotein), HDL (high density lipoproteins), LDL (low density lipoproteins), RICO (rats with increased cholesterol), TCA (trichloracetic acid)To read this article in full you will need to make a payment
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Article info
Publication history
Accepted:
February 17,
1995
Received in revised form:
January 27,
1995
Received:
January 5,
1994
Identification
Copyright
© 1995 Published by Elsevier Inc.
