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Abstract
We determined the effects of different dialysis conditions on the antioxidant content,
duration of the lag phase and oxidation rate of LDL. Dialysis for 22 h resulted in
a 56%–66% reduction in the concentrations of β-carotene, lycopene and α-tocopherol.
The lag phase of copper-induced oxidation of freshly isolated LDL was considerably
longer than that of LDL dialysed for 22 or 44 h. Our data show that dialysis may result
in LDL preparations with antioxidant compositions that are not truly representative
of freshly isolated lipoproteins.
Keywords
Abbreviations:
LDL (low density lipoprotein), LPO (lipoperoxides), MDA (malondialdehyde), PBS (phosphate-buffered saline), TAG (triacylglycerol)To read this article in full you will need to make a payment
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Article info
Publication history
Accepted:
February 23,
1995
Received:
December 20,
1994
Identification
Copyright
© 1995 Published by Elsevier Inc.
