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Abstract
Four polymorphic sites of the apolipoprotein B (apo B) gene were investigated by using
polymerase chain reaction (PCR) in 103 patients with coronary heart disease (CHD)
and 100 age-matched healthy individuals selected from a population of Han Chinese
in the Beijing area. The rare X + allele of the XbaI restriction site was more frequently
seen in CHD patients than in controls (0.088 vs. 0.025, P < 0.01). The relative frequency of rare E − allele of the EcoRI restriction site
was significantly higher in CHD patients compared with controls (0.11 vs. 0.04, P < 0.01). Similarly, 3′VNTR-L allele (number of repeat units > 39) at the VNTR region
was also present at an apparently higher frequency in CHD patients in comparison to
that in controls (0.602 vs. 0.290, P < 0.001). However, the difference in relative frequency of rare Del allele of the
Ins/Del polymorphism at the signal peptide was not significant between the two groups
(0.282 vs. 0.235, P > 0.05). In comparison with Caucasians, the relative frequencies of rare alleles
(Del, X + and E −) were found to be statistically lower in Han Chinese. Furthermore,
the Del and X + alleles, in linkage disequilibrium, were associated with a significantly
lower plasma level of HDL-C in CHD patients. Therefore it is suggested that genetic
variation within the apo B gene may exert some impact on lipid metabolism and contribute
to the susceptibility to development of CHD in Han Chinese.
Keywords
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Article info
Publication history
Accepted:
March 7,
1995
Received in revised form:
December 20,
1994
Received:
September 14,
1994
Identification
Copyright
© 1995 Published by Elsevier Inc.
