This paper is only available as a PDF. To read, Please Download here.
Abstract
Differentiation of human promyelocytic leukemic HL-60 cells with 1,25-dihydroxyvitamin
D3 (D3) results in macrophages which exhibit specific and saturable receptor-mediated processing
of both native and modified low density lipoprotein (LDL). Analysis of binding kinetics
demonstrated that macrophages bind LDL and acetyl-LDL with similar affinities, yet
possess significantly different numbers of receptors (55 ± 6 × 103 LDL receptors/cell vs. 79 ± 7 × 103 acetyl-LDL receptors/cell). D3-induced HL-60 macrophages challenged with LDL or acetyl-LDL exhibited suppression
of HMG-CoA reductase activity as well as a significant induction in the incorporation
of [14C]oleate into cholesteryl ester compared with macrophages incubated with lipoprotein
depleted serum. Maximum increases in ACAT activity were obtained in macrophages incubated
with 25-hydroxycholesterol plus LDL or acetyl-LDL. The increase in ACAT activity in
macrophages challenged with acetyl-LDL paralleled the increase in cellular cholesterol
content and the increase of oil red O lipid stainable material, imparting the macrophages
with a foamy appearance. The data indicate that D3-induced HL-60 macrophages are a useful model for the study of lipoprotein -macrophage
interactions as related to foam cell development and atherogenesis.
Keywords
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to AtherosclerosisAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- The role of altered lipoproteins in the pathogenesis of atherosclerosis.Am Heart J. 1987; 113: 573
- Factors regulating the activities of low density lipoprotein receptor and the scavenger receptor on human monocyte-macrophages.J Lipid Res. 1981; 22: 1131
- Modifications of low-density lipoprotein that increase atherogenicity.N Engl J Med. 1989; 320: 915
- Evidence that chylomicron remnants and B-VLDL are transported by the same receptor pathway in J774 murine macrophagederived cells.J Lipid Res. 1986; 27: 1062
- Lipoprotein metabolism in the macrophage. Implications for cholesterol deposition in atherosclerosis.Ann Rev Biochem. 1983; 52: 223
- Lipoprotein receptors of HL-60 macrophages: effect of differentiation with tetramyristic phorbol acetate and 1,25-dihydroxyvitamin D3.Arteriosclerosis Thromb. 1991; 11: 995
- Receptor-mediated endocytosis of low density lipoprotein in culture cell.Methods Enzymol. 1983; 98: 241
- Reversible accumulation of cholesteryl esters in macrophages incubated with acetylated lipoproteins.J Cell Biol. 1979; 82: 597
- The cholesteryl ester cycle in macrophage foam cells.J Biol Chem. 1980; 255: 9344
- Amplification of the gene for 3-hydroxy-3-methylglutaryl coenzyme A reductase, but not for 53-KDa protein, in UT-1 cells.J Biol Chem. 1983; 258: 8462
- Membrane-bound domain of HMG-CoA reductase is required for sterol-enhanced degradation of the enzyme.Cell. 1985; 41: 249
- cDNA cloning of the bovine low density lipoprotein receptor: Feedback regulation of a receptor mRNA.in: Proc Natl Acad Sci USA. 80. 1983: 7501
- Binding site on macrophages that mediates uptake and degradation of acetylated low density lipoprotein, producing massive cholesterol deposition.in: Proc Natl Acad Sci USA. 76. 1979: 333
- 1,25-Dihydroxyvitamin D3-induced differentiation in a human promyelocytic leukemia cell line (HL-60): receptor-mediated maturation to macrophage-like cells.J Cell Biol. 1984; 98: 391
- The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum.J Clin Invest. 1955; 34: 1345
- The isolation and purification of plasma lipoproteins.in: Laboratory Techniques in Biochemistry and Molecular Biology. A Guidebook to Lipoprotein Technique. Elsevier, New York1984: 55
- Degradation of cationized low density lipoprotein and regulation of cholesterol metabolism in homozygous familial hypercholesterolemia fibroblasts.in: Proc Natl Acad Sci USA. 73. 1976: 3178
- Properties and interpretation of the Woolf and Scatchard plots in analyzng data from steroid receptor assays.J Steroid Biochem. 1983; 19: 1407
- Divergence in cholesterol biosynthesis rates and 3(OH)-HMG-CoA reductase activity as a consequence of granulocyte versus monocyte-macrophage differentiation in HL-60 cells.in: Proc Natl Acad Sci USA. 81. 1984: 889
- Micro assay for 3-hydroxy-3-methylglutaryl CoA reductase in rat liver and in L-cell fibroblasts.Biochim Biophys Acta. 1974; 370: 369
- Acyl coenzyme A:cholesterol acyl transferase assay: silica gel column separation of reaction products.Anal Biochem. 1988; 173: 436
- Role of the kidney in the metabolism of plasma mevalonate. Studies in human and rhesus monkeys.J Clin Invest. 1985; 76: 31
- A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples.Anal Biochem. 1978; 87: 206
- Primer of Biostatistics.McGraw-Hill, New York1981
- The effect of mevalonate on 3-hydroxy-3-methylglutaryl-CoA reductase activity and the absolute rate of cholesterol biosynthesis in human monocyte-derived macrophage.Eur J Biochem. 1985; 153: 117
- The regulation of 3-hydroxy-3-methylglutaryl-CoA reductase activity, cholesterol esterification and the expression of low-density lipoprotein receptors in cultures of monocyte-derived macrophages.Biochem J. 1983; 210: 523
- Immunocytochemical analysis of cellular components in lesions. Use of monoclonal antibodies with the Watanable and fat-fed rabbit.Arteriosclerosis. 1986; 6: 601
- Characteristics of the cholesterol-esterifying activity in normal and atherosclerotic rabbit aortas.Circ Res. 1974; 34: 176
- Activity of acyl-CoA:cholesterol acyltransferase and 3-hydroxy-3-methylglutaryl-CoA reductase in subfractions of hepatic microsomes enriched with cholesterol.Biochim Biophys Acta. 1983; 754: 126
- Evidence indicating that inactivation of 3-hydroxy-3-methylglutaryl coenzyme A reductase by low density lipoproteins or by 25-hydroxycholesterol requires mediator protein(s) with rapid turnover rate.J Biol Chem. 1981; 256: 6174
- Properties of a solubilized and reconstituted preparation of acyl-CoA:cholesterol acyltransferase from rat liver.Biochim Biophys Acta. 1982; 710: 154
- Solubilization, partial purification, and reconstitution in phosphatidyl-cholesterol liposomes of acyl-CoA:cholesterol acyltransferase.Biochemistry. 1982; 21: 674
- 1,25-Dihydroxyvitamin D3-induced alterations of lipid metabolism in human monocyte-macrophages.Am J Physiol. 1989; 25: E290
- Alteration of lipid metabolism associated with the activation of mouse alveolar macrophages induced by 1a,25-dihydroxyvitamin D3.Endocrinology. 1987; 120: 1813
- Induction of acetylated low density lipoprotein receptor on the cells of human monocytic leukemia cell line (THP-1 cell).Biochem Biophys Res Commun. 1987; 146: 802
- Stanbury JB Wyngaarden JB Fredrickson DS Goldstein JL Brown MS The metabolic basis of inherited disease. McGraw-Hill, New York1983: 672
- Regulation of the low density lipoprotein receptor and hydroxymethylglutaryl coenzyme A reductase gene by protein kinase C and putative regulatory protein.in: Proc Natl Acad Sci USA. 861. 1989: 1133
- Metabolism of normal and modified low density lipoproteins by macrophage cell lines of murine and human origin.Biochim Biophys Acta. 1985; 833: 417
- Metabolic changes in LDL receptors and an appearance of scavenger receptors after phorbol ester-induced differentiation of U937 cells.Biochim Biophys Acta. 1991; 1082: 152
Article info
Publication history
Accepted:
March 14,
1995
Received in revised form:
March 9,
1995
Received:
December 20,
1994
Identification
Copyright
© 1995 Published by Elsevier Inc.
