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High affinity of a fucosylated chondroitin sulfate for plasma low density lipoprotein

  • Ana M.F Tovar
    Affiliations
    Departamento de Bioquímica Médica, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Caixa Postal 68041, Rio de Janeiro, RJ, 21941-590, Brazil

    Departamento de Patologia, Centro de Ciências Médicas, Universidade Federal Fluminense, Niterói, RJ, Brazil
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  • Paulo A.S Mourão
    Correspondence
    Corresponding author, Fax.: +55 21 270 8647; e-mail: mourã[email protected]
    Affiliations
    Departamento de Bioquímica Médica, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Caixa Postal 68041, Rio de Janeiro, RJ, 21941-590, Brazil
    Search for articles by this author

      Abstract

      Factors that influence the binding of sulfated polysaccharides to plasma low density lipoprotein (LDL) were investigated. Among the naturally occurring polysaccharides tested, a fucosylated chondroitin sulfate from an echinoderm exhibited the strongest interaction with LDL. Defucosylation and desulfation totally abolished the interaction with LDL while reduction of carboxyl groups had little effect. These data indicate that the sulfated fucose branches are essential for binding of fucosylated chondroitin sulfate to LDL. In addition, there was a positive correlation between the binding to LDL and increasing length of the sulfated polysaccharide chains. The possibility of a practical use of this fucosylated chondroitin sulfate for the binding of LDL is discussed.

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