Abstract
Background: Apolipoprotein (apo) E is a constituent of lipoproteins with considerable variation
due to cysteine–arginine exchanges. The apo E4 (Arg112-Cys) polymorphism has been
associated with dementia and hypercholesterolemia. We investigated the relation of
APOE genotype to cardiovascular disease (CVD) in the Framingham Offspring Study. Methods and results: DNA was isolated from 3413 study participants and APOE genotypes were determined
utilizing the polymerase chain reaction and restriction isotyping. In the entire group
of subjects, 20.7% had apo E4/4 or E3/4 (Group E4); 14.1% had apo E2/2 or E2/3 (Group
E2) and 63.9% had the apo E3/3 genotype (Group E3). Subjects with E2/4 (1.3%) were
excluded. Period prevalence of CVD between examinations 1 and 5 (1971–1994) (366 events)
was related to APOE genotype. Age adjusted period prevalence of CVD in men was 18.6%
for Group E4, 18.2% for Group E2 and 12.7% for Group E3 (P=0.004); while in women these rates were 9.9, 4.9, and 6.6%, respectively (P=0.037). After adjustment for non-lipid risk factors the relative odds for CVD in
Group E2 men was 1.79 (P=0.0098) and in Group E4 it was 1.63 (P=0.0086) compared with the Group E3; while in Group E4 women it was 1.56 (P=0.054). After adjustment for all CVD risk factors, the relative odds in Group E2
men was 1.94 (P=0.004) and in Group E4 men it was 1.51 (P=0.0262). Conclusions: The presence of the apo E2 or apo E4 alleles in men is associated with significantly
greater CVD risk. This genotypic information may help to identify individuals at increased
risk for CVD events.
Keywords
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Article info
Publication history
Accepted:
June 28,
2000
Received in revised form:
May 22,
2000
Received:
January 31,
2000
Identification
Copyright
© 2001 Elsevier Science Ireland Ltd. Published by Elsevier Inc. All rights reserved.
