Abstract
An association between a polymorphism of the angiotensin-converting enzyme (ACE) gene
and myocardial infarction (MI) in men has been previously reported. The present study
examines the association between ACE genotype, atherosclerosis, MI, hypertension and
other cardiovascular risk factors in Caucasian men (n=576) and women (n=124) who have undergone coronary angiography. Gene frequencies are also reported
for African–American men (n=56). Genotype determination was based on the presence (allele I) or absence (allele
D) of a 287 nucleotide Alu sequence in intron 16 of the ACE gene. Genotype frequencies for DD, ID and II were:
30.9, 47.7, 21.4% for Caucasian men; 28.2, 48.4, 23.4% for Caucasian women; and 30.4,
46.4, 23.2% for African–American men. There were no statistically significant associations
between ACE genotype and number of plaques (![Math Eq]()
10% obstruction), lipid variables, or body mass index (BMI) for Caucasian men. Caucasian
women with the DD genotype had on average fewer plaques, but this was accounted for
by their younger ages. In Caucasian males, the DD genotype independently contributed
to the presence of hypertension (odds ratio=1.8, 95% CI 1.1–2.9) after adjusting for
age and BMI. In Caucasian males with total cholesterol levels less than 200 mg/dl
(n=237), the DD (odds ratio=2.5, 95% CI 1.2–5.4) and ID genotypes (odds ratio=2.2, 95%
CI 1.1–4.4) were associated with a history of MI.
10% obstruction), lipid variables, or body mass index (BMI) for Caucasian men. Caucasian
women with the DD genotype had on average fewer plaques, but this was accounted for
by their younger ages. In Caucasian males, the DD genotype independently contributed
to the presence of hypertension (odds ratio=1.8, 95% CI 1.1–2.9) after adjusting for
age and BMI. In Caucasian males with total cholesterol levels less than 200 mg/dl
(n=237), the DD (odds ratio=2.5, 95% CI 1.2–5.4) and ID genotypes (odds ratio=2.2, 95%
CI 1.1–4.4) were associated with a history of MI.Keywords
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References
- Two putative active centres in human angiotensin I-converting enzyme revealed by molecular cloning.Proc. Natl. Acad. Sci. USA. 1988; 85: 9386-9390
- Angiotensin I-converting enzyme gene is on chromosome 17.Cytogenet. Cell. Genet. 1989; 51: 1041
- An insertion/deletion polymorphism in the angiotensin I-converting enzyme gene accounting for half the variance of serum enzyme levels.J. Clin. Invest. 1990; 86: 1343-1346
- Structure of the angiotensin I-converting enzyme gene: two alternate promoters correspond to evolutionary steps of a duplicated gene.J. Biol. Chem. 1991; 266: 15377-15383
- PCR detection of the insertion/deletion polymorphism of the human angiotensin-converting enzyme gene (DCP1) (dipeptidylcarboxypeptidase 1).Nucleic Acids Res. 1992; 20: 1433
- Differential effects of renin–angiotensin system blockade on atherogenesis in cholesterol-fed rabbits.J. Clin. Invest. 1993; 91: 1453-1458
- The ACE inhibitor alacepril suppresses atherogenesis independent of serum lipids in cholesterol-fed rabbits — critical analysis with new ultrasound technique.Jpn. Circ. J. 1994; 58: 844-854
- Antiatherosclerotic effects of the angiotensin-converting enzyme inhibitors captopril and fosinopril in hypercholesterolemic minipigs.J. Cardiovas. Pharmacol. 1994; 24: 670-677
- Trandolapril inhibits atherosclerosis in the Watanabe heritable hyperlipidemic rabbit.Hypertension. 1992; 20: 473-477
- Deletion polymorphism in the gene for angiotensin-converting enzyme is a potent risk factor for myocardial infarction.Nature. 1992; 359: 641-644
- A meta-analysis of the association of the deletion allele of the angiotensin-converting enzyme gene with myocardial infarction.Circulation. 1996; 94: 708-712
- Indications for ACE inhibitors in the early treatment of acute myocardial infarction: Systematic overview of individual data from 100 000 patients in randomized trials.Circulation. 1998; 97: 2202-2212
- Angiotensin-converting enzyme inhibitors.Circulation. 1998; 97: 1411-1420
- Evidence, from combined segregation and linkage analysis, that a variant of the angiotensin I-converting enzyme (ACE) gene controls plasma ACE levels.Am. J. Hum. Genet. 1992; 51: 197-205
- Angiotensin-converting enzyme gene polymorphism has no influence on the circulating renin–angiotensin–aldosterone system or blood pressure in normotensive subjects.Circulation. 1995; 91: 2933-2942
- The angiotensin I-converting enzyme gene and predisposition to high blood pressure.Hypertension. 1993; 21: 455-460
- Evidence for association and genetic linkage of the angiotensin-converting enzyme locus with hypertension and blood pressure in men but not women in the Framingham Heart Study.Circulation. 1998; 97: 1763-1765
- Influence of the angiotensin-converting enzyme I/D gene polymorphisms on left ventricular diastolic filling in patients with essential hypertension.J. Hypertens. 1997; 15: 995-1000
- Relationship of the angiotensin-converting enzyme gene polymorphism to glucose intolerance, insulin resistance, and hypertension in NIDDM.Hum. Genet. 1998; 102: 372-378
- Deletion polymorphism of the angiotensin-converting enzyme gene in patients with a history of ischaemic stroke.Arterioscler. Thromb. Vasc. Biol. 1996; 16: 304-309
- Angiotensin I-converting enzyme genotypes and left ventricular hypertrophy in patients with hypertrophic cardiomyopathy.Circulation. 1995; 92: 1808-1812
- Insertion/deletion polymorphism of the angiotensin-converting enzyme gene is strongly associated with coronary heart disease in non-insulin-dependent diabetes mellitus.Proc. Natl. Acad. Sci. USA. 1994; 91: 3662-3665
- Meta-analysis of association of insertion/deletion polymorphism of angiotensin I-converting enzyme gene with diabetic nephropathy and retinopathy.Diabetologia. 1998; 41: 47-53
- Angiotensin-converting enzyme gene polymorphism and cardiovascular disease.Clin. Sci. 1997; 93: 391-400
- The deletion/insertion polymorphism of the angiotensin-converting enzyme gene and cardiovascular–renal risk.J. Hypertens. 1997; 15: 1579-1592
Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd ed. New York: Cold Spring Harbor, 1989.
- Angiotensin I-converting enzyme genotype DD is a risk factor for coronary artery disease.J. Investig. Med. 1995; 43: 275-280
- Angiotensin-converting enzyme gene polymorphism is associated with myocardial infarction but not with development of coronary stenosis.Circulation. 1995; 91: 2120-2124
- The angiotensin-converting enzyme (ACE) genetic polymorphism: its relationship with plasma ACE level and myocardial infarction.Clin. Genet. 1994; 46: 94-101
- A prospective evaluation of an angiotensin-converting-enzyme gene polymorphism and the risk of ischemic heart disease.N. Engl. J. Med. 1995; 332: 706-711
- Effects of angiotensis II and vasopressin on human smooth muscle cells in vitro.Exp. Mol. Pathol. 1981; 35: 265-276
- Induction of platelet-derived growth factor A-chain and c-myc gene expressions by angiotensin II in cultured rat vascular smooth muscle cells.J. Clin. Invest. 1989; 83: 1419-1424
- Preservation of endothelial function by ramipril in rabbits on a long-term atherogenic diet.J. Cardiovas. Pharmacol. 1991; 18: S110-S115
- The II genotype of the angiotensin-converting enzyme gene delays the onset of acute coronary syndromes.Arterioscler. Thromb. Vasc. Biol. 1997; 17: 1730-1733
- Angiotensin-converting enzyme genotypes and risk for myocardial infarction in women.J. Am. Coll. Cardiol. 1998; 31: 790-796
- Gene polymorphism but not catalytic activity of angiotensin I-converting enzyme is associated with coronary artery disease and myocardial infarction in low-risk patients.Circulation. 1995; 92: 2796-2799
- A DNA variant at the angiotensin-converting enzyme gene locus associates with coronary artery disease in the Caerphilly Heart Study.Circulation. 1995; 91: 270-274
- Angiotensin-converting enzyme genotype is not associated with endothelial dysfunction in subjects without other coronary risk factors.Atherosclerosis. 1994; 111: 121-126
- Angiotensin-converting enzyme gene polymorphism is associated with endothelium-dependent vasodilation in never treated hypertensive patients.Circulation. 1998; 31: 900-905
- Polymorphisms of the angiotensin-converting-enzyme gene in subjects who die from coronary heart disease.Q. J. Med. 1994; 87: 211-214
- Angiotensin II induces smooth muscle cell proliferation in the normal and injured rat arterial wall.Circ. Res. 1991; 68: 450-456
- ACE inhibition, endothelial function and coronary artery lesions.Drugs. 1997; 54: 12-22
- Role of superoxide in angiotensin II-induced but not catecholamine-induced hypertension.Circulation. 1997; 95: 588-593
- Enhanced expression of angiotensin-converting enzyme is associated with progression of coronary atherosclerosis in humans.J. Hypertens. 1997; 15: 1295-1302
- Angiotensin I-converting enzyme gene: regulation, polymorphism and implications in cardiovascular diseases.Eur. Heart J. (Suppl. D). 1994; 15: 24-29
- The deletion/insertion polymorphism of the angiotensin-converting enzyme gene and cardiovascular–renal risk.J. Hypertens. 1997; 15: 1579-1592
Article info
Publication history
Accepted:
January 19,
2000
Received in revised form:
December 16,
1999
Received:
December 21,
1998
Identification
Copyright
© 2001 Elsevier Science Ireland Ltd. Published by Elsevier Inc. All rights reserved.
