Abstract
In a previous study it was shown that postprandial lipid metabolism is delayed in
individuals with intra-abdominal visceral fat accumulation. Population studies have
shown that as compared with individuals with apolipoprotein (apo) E3/3, those with
phenotype apo E3/4 phenotype have higher plasma and low density lipoprotein (LDL)-cholesterol
(C) concentration and increased susceptibility to coronary heart disease. The aim
of the present study is to determine how apo E4 affects postprandial lipid metabolism
by comparing individuals with apo E3/4 to those with apo E3/3 phenotype matched for
abdominal visceral fat. Sixty-two Japanese subjects (41 male, 21 female) [average
age 48±14 years; mean body mass index (BMI) 25±5.6 kg/m2] were recruited for this study. The subjects were divided into two groups: those
with apo E3/3 (n=43) and those with apo E3/4 phenotype (n=19), as determined by isoelectric focusing (IEF). Visceral fat accumulation was analyzed
as area of fat deposition by computerized tomography at the umbilicus level. After
a 12-h overnight fasting, an oral vitamin A and a fatty meal were administered to
these subjects. The plasma triglyceride (TG) increased significantly hours after fat
loading in both groups but the levels of TG were significantly higher in apo E3/4
than in apo E3/3 phenotype at 2, 4 and 6 h after fat loading. Plasma retinyl palmitate
(RP) levels were also significantly higher in individuals with apo E3/4 than in those
with apo E3/3 phenotype at 2, 4 and 6 h after fat loading. This investigation was
then conducted in both genders separately, and found that these associations were
statistically significant in men. Furthermore, after matching men for fasting TG levels,
these associations did not persist for plasma TG levels at any time point, while plasma
RP levels were still significantly higher in apo E3/4 group at 2 and 6 h after fat
loading. These results indicate that in Japanese population especially for men apo
E phenotype E3/4 is associated with an impaired postprandial TG-rich lipoprotein metabolism
relative to apo E3/3 phenotype when matched for intra-abdominal visceral fat accumulation,
which has a substantial effect on the metabolism of plasma TG-rich lipoproteins.
Keywords
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Article info
Publication history
Accepted:
March 2,
2000
Received in revised form:
February 4,
2000
Received:
August 3,
1999
Identification
Copyright
© 2001 Elsevier Science Ireland Ltd. Published by Elsevier Inc. All rights reserved.
