Variations in lipid and apolipoprotein concentrations in human leg lymph: effects of posture and physical exercise


      We studied the variations in the concentrations of cholesterol, triglycerides, phospholipids, apolipoproteins (apos) (A-I, A-II, B, C-III, E), free glycerol and albumin in human prenodal leg lymph during the 24 h cycle. Lymph was collected continuously for up to 96 h from nine healthy males on a low-fat isocaloric diet. In three free-living subjects, all lipid and apolipoprotein concentrations underwent synchronous variations, rising during the night and decreasing during the day. In three subjects who remained in supine rest for 48 h, the amplitude of circadian variation was much smaller. In three who alternated periods of supine rest with upright exercise, the highest concentrations occurred during rest. Lipid, apolipoprotein and albumin concentrations were inversely related to lymph flow rate. Free glycerol, much of which in tissue fluid is derived from local adipocytes, did not follow this pattern. On multiple regression, concentrations in lymph were related independently to the corresponding concentration in plasma (positive) and to lymph flow rate (negative) or lymph albumin concentration (positive). These results show that lipoprotein concentrations in human tissue fluid are determined only partly by their concentrations in plasma. They are also strongly affected by hemodynamic factors via their effects on fluid transport.


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        • Sloop C.H.
        • Dory L.
        • Roheim P.S.
        Interstitial fluid lipoproteins.
        J. Lipid Res. 1987; 28: 225-237
        • Reichl D.
        Lipoproteins of human peripheral lymph.
        Eur. Heart J. 1990; 11: 230-236
        • Michel C.C.
        Transport of macromolecules through microvascular walls.
        Cardiovasc. Res. 1996; 32: 644-653
        • Predescu D.
        • Predescu S.
        • McQuistan T.
        • Palade G.E.
        Transcytosis of alpha2-acid glycoprotein in the continuous microvascular endothelium.
        Proc. Natl. Acad. Sci. U.S.A. 1998; 95: 6175-6180
        • Szabo G.
        • Anda E.
        • Vandor E.
        The effect of muscle activity on the lymphatic and venous transport of lactate dehydrogenase.
        Lymphology. 1972; 5: 111-114
      1. Olszewski WL, editor. Lymph stasis: pathophysiology, diagnosis and treatment. Boston: CRC Press; 1991.

        • Olszewski W.L.
        • Engeset A.
        Intrinsic contractility of prenodal lymph vessels and lymph flow in human leg.
        Am. J. Physiol. 1980; 239: H775-H783
        • Rutili G.
        • Arfors K.-E.
        Protein concentration in interstitial and lymphatic fluids from the subcutaneous tissue.
        Acta Physiol. Scand. 1977; 99: 1-8
        • Nanjee M.N.
        • Cooke C.J.
        • Olszewski W.L.
        • Miller N.E.
        Lipid and apolipoprotein concentrations in prenodal leg lymph of fasted humans. Associations with plasma concentrations in normal subjects, lipoprotein lipase deficiency, and LCAT deficiency.
        J. Lipid Res. 2000; 41: 1317-1327
        • Engeset A.
        • Olszewski W.
        • Jaeger P.M.
        • Sokolowski J.
        • Theodorsen L.
        Twenty-four hour variation in flow and composition of leg lymph in normal men.
        Acta Physiol. Scand. 1977; 99: 140-148
        • Olszewski W.
        • Engeset A.
        • Sokolowski J.
        Lymph flow and protein in the normal male leg during lying, getting up and walking.
        Lymphology. 1977; 10: 178-183
        • Renkin E.M.
        • Joyner W.L.
        • Sloop C.H.
        • Watson P.D.
        Influence of venous pressure on plasma-lymph transport in the dog’s paw: convective and dissipative mechanisms.
        Microvasc. Res. 1977; 14: 191-204
        • Nanjee M.N.
        • Miller N.E.
        Sequential microenzymatic assay of cholesterol, triglycerides, and phospholipids in a single aliquot.
        Clin. Chem. 1996; 42: 915-926
        • Taylor A.
        • Gibson H.
        Concentrating ability of lymphatic vessels.
        Lymphology. 1975; 8: 43-49
        • Levick J.R.
        • Michel C.C.
        The effects of position and skin temperature on the capillary pressures in the fingers and toes.
        J. Physiol. (Lond.). 1978; 274: 97-109
        • Nanjee M.N.
        • Cooke C.J.
        • Wong J.
        • Hamilton R.L.
        • Olszewski W.L.
        • Miller N.E.
        Composition and ultrastructure of size subclasses of normal human peripheral lymph lipoproteins. Quantification of cholesterol uptake by HDLs in tissue fluids.
        J. Lipid Res. 2001; 42: 639-648
        • Reichl D.
        • Forte T.M.
        • Hong J.-L.
        • Rudra D.N.
        • Pflug J.
        Human lymphedema fluid lipoproteins: particle size, cholesterol and apolipoprotein distributions and electron microscopic structure.
        J. Lipid Res. 1985; 26: 1399-1411
        • Nanjee N.
        • Stepanova I.P.
        • Stocks J.
        • Cooke C.J.
        • Olszewski W.L.
        • Miller E.
        • et al.
        Oxidative modification of low density lipoproteins in normal human peripheral tissue fluid.
        Circulation. 2000; 102 ([abstract]): 11-283
        • Navab M.
        • Berliner J.A.
        • Watson A.D.
        • Hama S.Y.
        • Sevanian A.
        • Territo M.C.
        • et al.
        The Yin and Yang of oxidation in the development of the fatty streak.
        Arterioscler. Thromb. Vasc. Biol. 1996; 16: 831-842