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Influence of Chlamydia pneumoniae infection on aortic stiffness in healthy young men

      Abstract

      Though Chlamydia pneumoniae infection has been implicated in the pathogenesis of atherosclerosis, its role in early atherogenesis has not been well elucidated. To clarify whether C. pneumoniae infection was related to early atherogenesis, we evaluated the association between serological detection of C. pneumoniae antibodies and aortic stiffness in 102 healthy young male volunteers (mean age 27.1±0.4 years). Serum C. pneumoniae IgA and IgG antibodies were measured by the enzyme-linked immunosorbent assay (ELISA). Aortic stiffness was estimated using the brachial-ankle pulse wave velocity (PWV). No significant differences were observed between IgA seropositive and seronegative groups with regard to conventional cardiovascular risk factors. However, the mean PWV value was significantly higher in the IgA seropositive group than the seronegative group. Analyses of subgroups according to C-reactive protein (CRP) level showed that those subjects with IgA seropositivity and a high CRP level (>0.17 mg/l) had the highest PWV values. Multivariate logistic regression analysis revealed that a combination of C. pneumoniae IgA seropositivity and a high CRP level was an independent predictor of high values of PWV. These results suggest that C. pneumoniae infection might contribute to early atherogenesis, which might be associated with chronic inflammation.

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      References

        • Ross R.
        Atherosclerosis—an inflammatory disease.
        N. Engl. J. Med. 1999; 340: 115-126
        • Danesh J.
        • Collins R.
        • Peto R.
        Chronic infections and coronary heart disease: is there a link?.
        Lancet. 1997; 350: 430-436
        • Saikku P.
        • Leinonen M.
        • Mattila K.
        • Ekman M.R.
        • Nieminen M.S.
        • Makela P.H.
        • et al.
        Serological evidence of an association of a novel Chlamydia, TWAR, with chronic coronary heart disease and acute myocardial infarction.
        Lancet. 1988; 2: 983-986
        • Miyashita N.
        • Toyota E.
        • Sawayama T.
        • Matsumoto A.
        • Mikami Y.
        • Kawai N.
        • et al.
        Association of chronic infection of Chlamydia pneumoniae and coronary heart disease in the Japanese.
        Intern. Med. 1998; 37: 913-916
        • Schmidt C.
        • Hulthe J.
        • Wikstrand J.
        • Gnarpe H.
        • Gnarpe J.
        • Agewall S.
        • et al.
        Chlamydia pneumoniae seropositivity is associated with carotid artery intima-media thickness.
        Stroke. 2000; 31: 1526-1531
        • Sander D.
        • Winbeck K.
        • Klingelhofer J.
        • Etgen T.
        • Conrad B.
        Enhanced progression of early carotid atherosclerosis is related to Chlamydia pneumoniae (Taiwan acute respiratory) seropositivity.
        Circulation. 2001; 103: 1390-1395
        • Kuo C.C.
        • Shor A.
        • Campbell L.A.
        • Fukushi H.
        • Patton D.L.
        • Grayston J.T.
        Demonstration of Chlamydia pneumoniae in atherosclerotic lesions of coronary arteries.
        J. Infect. Dis. 1993; 167: 841-849
        • Davidson M.
        • Kuo C.C.
        • Middaugh J.P.
        • Campbell L.A.
        • Wang S.P.
        • Newman III, W.P.
        • et al.
        Confirmed previous infection with Chlamydia pneumoniae (TWAR) and its presence in early coronary atherosclerosis.
        Circulation. 1998; 98: 628-633
        • Rassu M.
        • Cazzavillan S.
        • Scagnelli M.
        • Peron A.
        • Bevilacqua P.A.
        • Facco M.
        • et al.
        Demonstration of Chlamydia pneumoniae in atherosclerotic arteries from various vascular regions.
        Atherosclerosis. 2001; 158: 73-79
        • Muhlestein J.B.
        • Anderson J.L.
        • Hammond E.H.
        • Zhao L.
        • Trehan S.
        • Schwobe E.P.
        • et al.
        Infection with Chlamydia pneumoniae accelerates the development of atherosclerosis and treatment with azithromycin prevents it in a rabbit model.
        Circulation. 1998; 97: 633-636
        • Celermajer D.S.
        • Sorensen K.E.
        • Gooch V.M.
        • Spiegelhalter D.J.
        • Miller O.I.
        • Sullivan I.D.
        • et al.
        Non-invasive detection of endothelial dysfunction in children and adults at risk of atherosclerosis.
        Lancet. 1992; 340: 1111-1115
        • Blacher J.
        • Asmar R.
        • Djane S.
        • London G.M.
        • Safar M.E.
        Aortic pulse wave velocity as a marker of cardiovascular risk in hypertensive patients.
        Hypertension. 1999; 33: 1111-1117
        • Yamashina A.
        • Tomiyama H.
        • Takeda K.
        • Tsuda H.
        • Arai T.
        • Hirose K.
        • et al.
        Validity, reproducibility and clinical significance of noninvasive brachial-ankle pulse wave velocity measurement.
        Hypertens. Res. 2002; 25: 359-364
        • Kishimoto T.
        • Kubota Y.
        • Matsushima T.
        • Izutsu H.
        • Matsumoto A.
        • Soejima R.
        • et al.
        Assay of specific anti-Chlamydia pneumoniae antibodies by ELISA method. 1. Evaluation of ELISA kit using outer membrane complex.
        Kansenshogaku Zasshi. 1996; 70 (article in Japanese, abstract in English): 821-829
        • Kishimoto T.
        • Kubota Y.
        • Matsushima T.
        • Izutsu H.
        • Matsumoto A.
        • Soejima R.
        • et al.
        Assay of specific anti-Chlamydia pneumoniae antibodies by ELISA method. 2. Studies on clinical usefulness and serological diagnostic standards.
        Kansenshogaku Zasshi. 1996; 70 (article in Japanese, abstract in English): 830-839
        • Numazaki K.
        • Ikebe T.
        • Chiba S.
        Detection of serum antibodies against Chlamydia pneumoniae by ELISA.
        FEMS Immunol. Med. Microbiol. 1996; 14: 179-183
        • Miyashita N.
        • Fukano H.
        • Yoshida K.
        • Niki Y.
        • Matsushima T.
        Seroepidemiology of Chlamydia pneumoniae in Japan between 1991 and 2000.
        J. Clin. Pathol. 2002; 55: 115-117
        • Grayston J.T.
        • Kuo C.C.
        • Wang S.P.
        • Altman J.
        A new Chlamydia psittaci strain TWAR isolated in acute respiratory tract infections.
        N. Engl. J. Med. 1986; 315: 161-168
        • Saikku P.
        • Leinonen M.
        • Tenkanen L.
        • Linnanmaki E.
        • Ekman M.R.
        • Manninen V.
        • et al.
        Chronic Chlamydia pneumoniae infection as a risk factor for coronary heart disease in the Helsinki Heart Study.
        Ann. Intern. Med. 1992; 116: 273-278
        • Markus H.S.
        • Sitzer M.
        • Carrington D.
        • Mendall M.A.
        • Steinmetz H.
        Chlamydia pneumoniae infection and early asymptomatic carotid atherosclerosis.
        Circulation. 1999; 100: 832-837
        • Moazed T.C.
        • Campbell L.A.
        • Rosenfeld M.E.
        • Grayston J.T.
        • Kuo C.C.
        Chlamydia pneumoniae infection accelerates the progression of atherosclerosis in apolipoprotein E-deficient mice.
        J. Infect. Dis. 1999; 180: 238-241
        • Godzik K.L.
        • O’Brien E.R.
        • Wang S.K.
        • Kuo C.C.
        In vitro susceptibility of human vascular wall cells to infection with Chlamydia pneumoniae.
        J. Clin. Microbiol. 1995; 33: 2411-2414
        • Gaydos C.A.
        • Summersgill J.T.
        • Sahney N.N.
        • Ramirez J.A.
        • Quinn T.C.
        Replication of Chlamydia pneumoniae in vitro in human macrophages endothelial cells and aortic artery smooth muscle cells.
        Infect. Immun. 1996; 64: 1614-1620
        • Dechend R.
        • Maass M.
        • Gieffers J.
        • Dietz R.
        • Scheidereit C.
        • Leutz A.
        • et al.
        Chlamydia pneumoniae infection of vascular smooth muscle and endothelial cells activates NF-kappaB and induces tissue factor and PAI-1 expression: a potential link to accelerated arteriosclerosis.
        Circulation. 1999; 100: 1369-1373
        • Heinemann M.
        • Susa M.
        • Simnacher U.
        • Marre R.
        • Essig A.
        Growth of Chlamydia pneumoniae induces cytokine production and expression of CD14 in a human monocytic cell line.
        Infect. Immun. 1996; 64: 4872-4875
        • Kol A.
        • Sukhova G.K.
        • Lichtman A.H.
        • Libby P.
        Chlamydial heat shock protein 60 localizes in human atheroma and regulates macrophage tumor necrosis factor-alpha and matrix metalloproteinase expression.
        Circulation. 1998; 98: 300-307
        • Maeda N.
        • Sawayama Y.
        • Tatsukawa M.
        • Shimizu C.
        • Kashiwagi S.
        • Hayashi J.
        Chlamydia pneumoniae seropositivity and early carotid atherosclerosis in a suburban Japanese population.
        Atherosclerosis. 2002; 164: 313-319
      1. Relationship of atherosclerosis in young men to serum lipoprotein cholesterol concentrations and smoking. A preliminary report from the Pathobiological Determinants of Atherosclerosis in Youth (PDAY) Research Group. J Am Med Assoc 1990;264:3018–24.

        • Ridker P.M.
        • Cushman M.
        • Stampfer M.J.
        • Tracy R.P.
        • Hennekens C.H.
        Inflammation, aspirin, and the risk of cardiovascular disease in apparently healthy men.
        N. Engl. J. Med. 1997; 336: 973-979
        • Danesh J.
        Smoldering arteries? Low-grade inflammation and coronary heart disease.
        J. Am. Med. Assoc. 1999; 282: 2169-2171