Abstract
There is accumulating evidence that LDL oxidation is essential for atherogenesis,
and that antioxidants that prevent this oxidation may either slow down or prevent
atherogenesis. In the present study, we found that Commiphora mukul and its cholesterol-lowering component, guggulsterone, effectively inhibited LDL
oxidation mediated by either catalytic copper ions, free radicals generated with the
azo compound 2,2′-azobis-(2-amidinopropane)dihydrochloride (AAPH), soybean lipoxygenase
enzymatically, or mouse peritoneal macrophages. This inhibition was assessed by the
decrease in the following parameters describing LDL oxidation: conjugated dienes,
relative electrophoretic mobility (REM), thiobarbituric acid reactive substances,
lipid hydroperoxides, oxidation-specific immune epitopes as detected with a monoclonal
antibody against oxidized LDL, and the accumulation of LDL derived cholesterol esters
in mouse peritoneal macrophages. We concluded that C. mukul and its lipid-lowering component, guggulsterone, significantly inhibit LDL oxidation.
The combination of antioxidant and lipid-lowering properties of C. mukul and guggulsterone makes them especially beneficial against atherogenesis.
Keywords
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Article info
Publication history
Accepted:
October 30,
2003
Received in revised form:
September 15,
2003
Received:
June 16,
2003
Identification
Copyright
© 2003 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
