Abstract
This study was designed to address the effects of a moderate consumption of beer on
serum and liver lipid parameters and on the development of aortic lesions in a mouse
model associated with a human atherogenic lipoprotein profile. LDLr−/− apoB100/100 mice received each day during 12 weeks either water, mild beer (0.570 g of ethanol/kg of body weight) or ethanol-free beer in a single pure dose. Serum
and liver lipid parameters were analyzed and atherosclerotic lesions were estimated
in heart and aorta through their total cholesterol content. mRNA levels of enzymes
and receptors involved in lipoprotein uptake, in fatty acid esterification and oxidation,
and in reverse cholesterol transport were also measured in the liver. Serum glucose,
triglyceride (TG) and cholesterol levels were altered neither by ethanol-free beer
nor by mild beer. Nevertheless, both beer treatments significantly increased HDL-cholesterol
(HDL-C) and VLDL-C levels by reference to controls with no change in LDL-C levels.
Liver TG contents were significantly decreased by either beer treatment. Cholesterol
accumulation was attenuated in the whole aorta of mice treated with mild beer at p < 0.05 and not significantly with ethanol-free beer. Heart cholesterol contents were
comparable in the three series. Among the genes studied, only scavenger receptor-B1
was downregulated by both beer-based beverages. LDL receptor related protein, lecithin–cholesterol
acyltransferase and sterol regulatory element-binding protein 2 were downregulated
only by mild beer. The expression of other genes assayed was not altered. When administered
in chronic and moderate dose, unidentified components of beer may exert beneficial
effects towards atherosclerosis development through alteration of lipoprotein metabolism
in LDLr−/− apoB100/100 mice. This effect was slightly amplified by the presence of ethanol in beer.
Keywords
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Article Info
Publication History
Published online: February 17, 2006
Accepted:
January 16,
2006
Received in revised form:
January 11,
2006
Received:
October 19,
2005
Identification
Copyright
© 2006 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
