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Letter to the Editor| Volume 187, ISSUE 1, P213-214, July 2006

LDL fractions assessed by anion-exchange high-performance liquid chromatography in patients with coronary artery disease

Published:April 17, 2006DOI:https://doi.org/10.1016/j.atherosclerosis.2006.03.014
LDL fractions assessed by anion-exchange high-performance liquid chromatography in patients with coronary artery disease
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      LDL oxidation plays a key role in the development of atherosclerosis. Several ELISA methods had been developed to measure oxidatively modified LDL levels in blood. Plasma oxidized LDL (OxLDL) levels were measured by ELISA using monoclonal antibodies against copper OxLDL [
      • Holvoet P.
      • Mertens A.
      • Verhamme P.
      • et al.
      Circulating oxidized LDL is a useful marker for identifying patients with coronary artery disease.
      Arterioscler Thromb Vasc Biol. 2001; 21: 844-848
      ] or oxidized phosphatidyl-choline [
      • Toshima S.
      • Hasegawa A.
      • Kurabayashi M.
      • et al.
      Circulating oxidized low density lipoprotein levels: a biochemical risk marker for coronary heart disease.
      Arterioscler Thromb Vasc Biol. 2000; 20: 2243-2247
      ]. Serum malondialdehyde-modified LDL (MDA-LDL) levels were also measured by ELISA using monoclonal antibody against MDA-LDL [
      • Tanaga K.
      • Bujo H.
      • Inoue M.
      • et al.
      Increased circulating malondialdehyde-modified LDL levels in patients with coronary artery diseases and their association with peak sizes of LDL particles.
      Arterioscler Thromb Vasc Biol. 2002; 22: 662-666
      ]. Using these methods, OxLDL and MDA-LDL levels were demonstrated to be high in patients with coronary artery disease (CAD) [
      • Holvoet P.
      • Mertens A.
      • Verhamme P.
      • et al.
      Circulating oxidized LDL is a useful marker for identifying patients with coronary artery disease.
      Arterioscler Thromb Vasc Biol. 2001; 21: 844-848
      ,
      • Toshima S.
      • Hasegawa A.
      • Kurabayashi M.
      • et al.
      Circulating oxidized low density lipoprotein levels: a biochemical risk marker for coronary heart disease.
      Arterioscler Thromb Vasc Biol. 2000; 20: 2243-2247
      ,
      • Tanaga K.
      • Bujo H.
      • Inoue M.
      • et al.
      Increased circulating malondialdehyde-modified LDL levels in patients with coronary artery diseases and their association with peak sizes of LDL particles.
      Arterioscler Thromb Vasc Biol. 2002; 22: 662-666
      ]. Recently, the anion-exchange high-performance liquid chromatography (AE-HPLC) method was reported to assess OxLDL levels. By this AE-HPLC method, three LDL fractions are detected, and LDL-3, which is the most negatively charged one, is considered to reflect OxLDL [
      • Yamaguchi Y.
      • Kagota S.
      • Kunitomo M.
      • Haginaka J.
      Evidence of modified lipoprotein in the plasma of Watanabe heritable hyperlipidemic rabbits by anion-exchange high-performance liquid chromatographic assay.
      Atherosclerosis. 1998; 139: 323-331
      ,
      • Yamaguchi Y.
      • Haginaka J.
      • Morimoto S.
      • Fujioka Y.
      • Kunitomo M.
      Facilitated nitration and oxidation of LDL in cigarette smokers.
      Eur J Clin Invest. 2005; 35: 186-193
      ]. In 2005, Matsuoka et al. [
      • Matsuoka H.
      • Yamagishi S.
      • Umei H.
      • Kitano S.
      • Kawano K.
      • Tamoto Y.
      Novel fraction of LDL as a risk factor for metabolic syndrome.
      Circulation. 2005; 112 (Abstract): II-209
      ] reported increased LDL-3 levels to be associated with metabolic syndrome and also to be associated with increased carotid intima-media thickness (IMT). Therefore, we expected that LDL-3 would be a biochemical marker for CAD.

      Keywords

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      References

        • Holvoet P.
        • Mertens A.
        • Verhamme P.
        • et al.
        Circulating oxidized LDL is a useful marker for identifying patients with coronary artery disease.
        Arterioscler Thromb Vasc Biol. 2001; 21: 844-848
        View in Article
        • Toshima S.
        • Hasegawa A.
        • Kurabayashi M.
        • et al.
        Circulating oxidized low density lipoprotein levels: a biochemical risk marker for coronary heart disease.
        Arterioscler Thromb Vasc Biol. 2000; 20: 2243-2247
        View in Article
        • Tanaga K.
        • Bujo H.
        • Inoue M.
        • et al.
        Increased circulating malondialdehyde-modified LDL levels in patients with coronary artery diseases and their association with peak sizes of LDL particles.
        Arterioscler Thromb Vasc Biol. 2002; 22: 662-666
        View in Article
        • Yamaguchi Y.
        • Kagota S.
        • Kunitomo M.
        • Haginaka J.
        Evidence of modified lipoprotein in the plasma of Watanabe heritable hyperlipidemic rabbits by anion-exchange high-performance liquid chromatographic assay.
        Atherosclerosis. 1998; 139: 323-331
        View in Article
        • Yamaguchi Y.
        • Haginaka J.
        • Morimoto S.
        • Fujioka Y.
        • Kunitomo M.
        Facilitated nitration and oxidation of LDL in cigarette smokers.
        Eur J Clin Invest. 2005; 35: 186-193
        View in Article
        • Matsuoka H.
        • Yamagishi S.
        • Umei H.
        • Kitano S.
        • Kawano K.
        • Tamoto Y.
        Novel fraction of LDL as a risk factor for metabolic syndrome.
        Circulation. 2005; 112 (Abstract): II-209
        View in Article
        • Ndrepepa G.
        • Braun S.
        • von Beckerath N.
        • et al.
        Oxidized low density lipoproteins, statin therapy and severity of coronary artery disease.
        Clin Chim Acta. 2005; 360: 178-186
        View in Article
        • Ehara S.
        • Ueda M.
        • Naruko T.
        • et al.
        Elevated levels of oxidized low-density lipoprotein show a positive relationship with the severity of acute coronary syndromes.
        Circulation. 2001; 103: 1955-1960
        View in Article

      Article info

      Publication history

      Published online: April 17, 2006
      Received: March 2, 2006

      Identification

      DOI: https://doi.org/10.1016/j.atherosclerosis.2006.03.014

      Copyright

      © 2006 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.

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